Detailed information    

insolico Bioinformatically predicted

Overview

Name   comB4   Type   Machinery gene
Locus tag   CV730_RS00860 Genome accession   NZ_CP024946
Coordinates   174793..177156 (+) Length   787 a.a.
NCBI ID   WP_145728126.1    Uniprot ID   -
Organism   Helicobacter pylori strain B147     
Function   transformation-associated type IV transport system (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 169793..182156
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CV730_RS00825 (CV730_00820) groES 169809..170165 (-) 357 WP_120824060.1 co-chaperone GroES -
  CV730_RS00835 (CV730_00830) dnaG 170453..172132 (+) 1680 WP_145728123.1 DNA primase -
  CV730_RS00840 (CV730_00835) - 172129..173181 (+) 1053 WP_145728124.1 MnmA/TRMU family protein -
  CV730_RS00845 (CV730_00840) - 173274..174101 (+) 828 WP_145728125.1 DUF5718 family protein -
  CV730_RS00850 (CV730_00845) comB2 174247..174528 (+) 282 WP_001272690.1 TrbC/VirB2 family protein Machinery gene
  CV730_RS00855 (CV730_00850) comB3 174528..174791 (+) 264 WP_000584956.1 hypothetical protein Machinery gene
  CV730_RS00860 (CV730_00855) comB4 174793..177156 (+) 2364 WP_145728126.1 VirB4 family type IV secretion/conjugal transfer ATPase Machinery gene
  CV730_RS00870 (CV730_00865) - 177460..178800 (+) 1341 WP_194151273.1 COG3014 family protein -
  CV730_RS00875 (CV730_00870) cheV1 178908..179873 (+) 966 WP_120865460.1 chemotaxis protein CheV1 -
  CV730_RS00880 (CV730_00875) nspC 179870..181087 (+) 1218 WP_145728128.1 carboxynorspermidine decarboxylase -
  CV730_RS00885 (CV730_00880) lpxE 181097..181630 (-) 534 WP_227979724.1 lipid A 1-phosphatase LpxE -

Sequence

Protein


Download         Length: 787 a.a.        Molecular weight: 90252.77 Da        Isoelectric Point: 8.7123

>NTDB_id=256532 CV730_RS00860 WP_145728126.1 174793..177156(+) (comB4) [Helicobacter pylori strain B147]
MLEKLLSTVKQKVSNYFLGVLPKSYSMSEENNILGLYDEHFLLTKNENLVGVLRLEGVSYTHLSTEQLQDLFTERQMALD
SLEKVVARLVVKRRKIDYQQNIQSDSQYLQAILNQFENKEVYENQYFLVLESAHSLQGVLEHKKKSLMHANRENFKDILS
YKAHFLQETLKSLEIQLKNYAPKLLNSKEVLNFYAEYINGFDLPLKPLVGGYLSDSYIASSITFEKDYFIQESFNQKTYN
RLIGIKAYESERITSIAVGALLYQETPLDIIFSIEPMSVHKTLSFLKERAKFSMSNLVKNELLEYQELVKTKRLSMQKFA
LNVLVKAPSLEDLDAQTSLILGLLFKENLVGVIETFGLKGGYFSFFPERIHLNHRLRFLTSKALACLMVFERQNLGFKAN
SWGNSPLSVFKNLDYSPFLFNFHNQEVSHNNAKEIARVNGHTLIIGATGSGKSTLISFLMMSALKYQNMRLLAFDRMQGL
YSFTKFFKGHYHDGKSFSINPFCLEPNLQNLEFLQSFFLSMLDLAPSRDKEALEDMNAVFGAIKSLYETLYPKAFSLLDF
KETLKRTSSNQLSLSLEPYLNNPLFNALNDAFNSNAFLNVINLDAITQNPKDLGLLAYYLFYKILEESRKNDSGFLVFLD
EFKSYVENDLLNTKINALITQARKANGVVVLALQDIYQLSGVKNAHSFLSNMGTLILYPQKNARELKHNFNVPLSETEIS
FLENTPLYARQVLVKNLGNGNSNMIDVSLESLGRYLKIFNSDSSHVNKVKALQKDYPTEWREKLLKS

Nucleotide


Download         Length: 2364 bp        

>NTDB_id=256532 CV730_RS00860 WP_145728126.1 174793..177156(+) (comB4) [Helicobacter pylori strain B147]
ATGTTAGAAAAGCTTTTGAGCACTGTCAAACAAAAGGTTTCAAACTATTTTTTAGGGGTTTTGCCTAAAAGCTATTCTAT
GAGCGAAGAAAACAACATTTTAGGCTTGTATGATGAGCATTTTTTACTCACTAAAAACGAAAACTTAGTGGGCGTCCTCC
GTTTAGAGGGGGTGAGTTACACCCATTTAAGCACAGAACAATTGCAAGATCTTTTCACTGAACGCCAAATGGCGTTGGAT
TCTTTAGAAAAGGTCGTGGCGCGCCTGGTGGTTAAAAGGCGTAAAATTGATTACCAACAAAACATTCAATCTGATTCTCA
ATACTTGCAAGCGATTTTGAATCAATTTGAAAATAAAGAAGTGTATGAGAATCAGTATTTTTTAGTTTTAGAAAGCGCGC
ATTCTTTACAAGGCGTTTTAGAACATAAGAAAAAATCTCTCATGCATGCTAATAGGGAAAATTTTAAGGATATTCTCTCT
TATAAAGCGCATTTTTTGCAAGAAACTTTAAAAAGCTTAGAAATCCAGCTCAAAAACTATGCCCCCAAACTCTTAAATTC
TAAAGAGGTTTTGAATTTTTATGCAGAATACATTAACGGGTTTGATCTCCCCTTAAAACCCCTAGTAGGAGGGTATTTGA
GCGATAGCTATATCGCTAGTTCTATCACTTTTGAAAAAGATTATTTCATTCAAGAAAGCTTTAACCAAAAAACCTATAAC
CGCTTGATTGGCATTAAAGCTTATGAGAGCGAAAGGATCACTTCTATAGCGGTGGGAGCGCTTTTATACCAAGAGACTCC
ACTAGATATTATCTTTTCTATAGAGCCTATGAGTGTGCATAAAACGCTGAGTTTTTTAAAAGAGAGGGCCAAATTTAGCA
TGTCCAATCTCGTTAAAAACGAGCTTTTAGAATACCAAGAATTAGTCAAAACTAAACGATTATCCATGCAAAAATTCGCC
CTAAACGTTCTTGTCAAAGCCCCCAGTTTAGAGGATTTAGACGCTCAAACCAGCTTAATTTTAGGGCTTTTATTTAAAGA
AAACTTAGTGGGCGTTATAGAAACTTTTGGCTTGAAAGGGGGGTATTTTTCCTTTTTCCCTGAACGCATCCATTTAAACC
ACCGCTTGCGTTTTTTAACCTCTAAAGCCCTAGCGTGTTTAATGGTGTTTGAAAGGCAAAATTTAGGTTTTAAGGCTAAT
TCATGGGGGAATAGCCCTTTGAGCGTGTTTAAAAATTTGGATTATTCCCCTTTTTTATTCAATTTCCACAACCAAGAAGT
GAGCCACAACAACGCTAAAGAAATCGCCAGAGTGAATGGGCATACTCTAATCATAGGGGCTACCGGGAGCGGTAAAAGCA
CGCTGATTAGCTTTTTAATGATGAGCGCTTTGAAATACCAAAACATGCGCCTTTTAGCTTTTGACAGGATGCAAGGGTTG
TATTCTTTCACAAAATTTTTTAAAGGGCATTACCATGACGGCAAATCTTTTAGCATCAACCCCTTTTGTTTAGAACCTAA
TTTGCAGAATTTAGAATTTTTGCAATCCTTCTTTTTGAGCATGTTGGATCTCGCCCCTTCAAGGGATAAAGAAGCCTTAG
AAGACATGAATGCGGTTTTTGGCGCGATTAAGAGCCTTTATGAGACTTTATACCCTAAAGCCTTTAGTTTGCTAGACTTT
AAAGAAACGCTTAAAAGAACCTCATCTAACCAACTAAGCTTGAGCTTGGAGCCGTATTTGAATAACCCCCTTTTTAACGC
TTTGAATGACGCGTTCAATTCCAACGCTTTTTTAAATGTAATCAACTTAGACGCTATCACCCAAAACCCTAAAGACTTAG
GGCTTTTAGCCTATTATTTGTTTTATAAAATTTTAGAAGAATCCAGGAAAAACGACAGCGGGTTTTTGGTTTTTTTAGAC
GAGTTTAAATCTTATGTGGAAAACGATTTGCTGAACACCAAAATCAACGCTTTAATCACGCAAGCCAGAAAAGCTAATGG
CGTGGTGGTGTTGGCCTTGCAAGATATTTACCAATTAAGCGGGGTTAAAAACGCCCATAGTTTTTTAAGCAACATGGGGA
CTCTCATTTTGTATCCGCAAAAAAACGCTAGGGAGTTGAAACACAATTTCAATGTGCCTTTGAGCGAGACTGAAATTTCT
TTTTTAGAAAACACCCCTTTGTATGCCAGGCAGGTTTTAGTCAAAAATCTGGGTAACGGGAATTCTAACATGATTGATGT
GAGTTTGGAAAGTTTAGGGCGTTATTTGAAAATCTTTAATTCAGATTCTAGTCATGTTAATAAAGTGAAAGCGTTACAAA
AAGACTACCCTACAGAGTGGCGTGAGAAACTTTTGAAGAGTTAG

Domains

Predicted by InterproScan.

FtsK/SpoIIIE family (FtsK_SpoIIIE)

(426-466)

CagE, TrbE, VirB family, component of type IV transporter system (CagE_TrbE_VirB)

(176-375)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comB4 Helicobacter pylori 26695

97.078

100

0.971

Multiple sequence alignment