Detailed information NTG0025649

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: MFLA_RS03755
• Genome accession: NC_007947
• Coordinates: 764165..765172 (-)
• Length: 335 a.a.
• NCBI ID: WP_011479098.1
• Uniprot ID: Q1H3D6
• Organism: Methylobacillus flagellatus KT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 759165..770172

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MFLA_RS03730 (Mfla_0728)	-	759591..760520 (+)	930	WP_011479093.1	electron transfer flavoprotein subunit alpha/FixB family protein	-
MFLA_RS03735 (Mfla_0729)	-	760552..761328 (+)	777	WP_011479094.1	ferredoxin--NADP reductase	-
MFLA_RS03740 (Mfla_0730)	-	761475..762530 (-)	1056	WP_011479095.1	hypothetical protein	-
MFLA_RS03745 (Mfla_0731)	ftsX	762560..763459 (-)	900	WP_011479096.1	permease-like cell division protein FtsX	-
MFLA_RS03750 (Mfla_0732)	ftsE	763456..764112 (-)	657	WP_011479097.1	cell division ATP-binding protein FtsE	-
MFLA_RS03755 (Mfla_0733)	pilA	764165..765172 (-)	1008	WP_011479098.1	signal recognition particle-docking protein FtsY	Machinery gene
MFLA_RS03760 (Mfla_0734)	-	765501..766820 (+)	1320	WP_048811842.1	M16 family metallopeptidase	-
MFLA_RS03765 (Mfla_0735)	-	766810..768150 (+)	1341	WP_011479100.1	M16 family metallopeptidase	-
MFLA_RS03770 (Mfla_0736)	rsmD	768137..768694 (+)	558	WP_011479101.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
MFLA_RS03775 (Mfla_0737)	coaD	768734..769216 (+)	483	WP_011479102.1	pantetheine-phosphate adenylyltransferase	-
MFLA_RS14330 (Mfla_0738)	-	769236..769493 (+)	258	WP_011479103.1	YfhL family 4Fe-4S dicluster ferredoxin	-

Sequence

Protein

• Length: 335 a.a.
• Molecular weight: 36174.43 Da
• Isoelectric Point: 5.9458

>NTDB_id=25649 MFLA_RS03755 WP_011479098.1 764165..765172(-) (pilA) [Methylobacillus flagellatus KT]
MFDFFKKNKPEDPPTPAAQDPVPAPQPLTQEKPSLSWAERLKRSLAKTRSQWGQQLARLFGGGKIDENVYEELETILLTS
DVGIAATQALLEQIRTRVKRQNLSDTTQLKEALKQSLLELLTPLEKPLDTTTAQPFVIMLAGVNGAGKTTTIGKLANLFQ
AQGKSVLIAAGDTFRAAAREQLQAWGERNNVHVVAQESGDPAAVIFDAVNSAKARGIDIVLADTAGRLPTQLNLMEEIRK
VQRVIDKALPGAPHEVLLVLDSNTGQNAVTQVKAFDDALGVTGLVLSKLDGTAKGGVIAAIAQSRPVPIRYIGIGESIDD
LRPFKASEFIDALFE

Nucleotide

• Length: 1008 bp

>NTDB_id=25649 MFLA_RS03755 WP_011479098.1 764165..765172(-) (pilA) [Methylobacillus flagellatus KT]
ATGTTCGACTTTTTCAAGAAAAACAAGCCTGAGGATCCACCTACGCCAGCGGCGCAAGACCCTGTCCCTGCGCCGCAGCC
GCTAACCCAGGAAAAGCCTTCCTTGAGCTGGGCGGAGCGGCTCAAGCGCAGCCTGGCCAAGACACGCAGCCAATGGGGCC
AGCAACTGGCACGCCTGTTCGGTGGCGGTAAGATTGACGAAAACGTTTACGAGGAGCTCGAAACCATCCTGCTGACCTCT
GACGTGGGCATCGCCGCCACCCAGGCGCTGCTGGAGCAGATCCGCACGCGCGTGAAACGTCAGAACCTCAGCGACACCAC
CCAGCTCAAGGAAGCACTCAAGCAATCCCTGCTGGAGCTGCTCACCCCGCTGGAGAAGCCGCTGGATACCACCACCGCGC
AACCTTTCGTCATCATGCTGGCCGGTGTCAATGGTGCAGGCAAGACGACGACCATAGGCAAACTCGCCAACTTGTTCCAG
GCCCAGGGCAAGTCCGTCCTGATCGCAGCGGGTGATACTTTCCGCGCTGCGGCACGCGAGCAGCTGCAGGCCTGGGGCGA
GCGCAACAACGTGCACGTGGTTGCTCAGGAGAGCGGCGACCCCGCTGCCGTGATTTTCGACGCCGTCAATTCGGCCAAGG
CGCGCGGCATCGATATCGTGCTGGCAGACACCGCAGGCCGGCTACCCACACAGCTCAACCTCATGGAGGAGATCCGCAAA
GTGCAGCGCGTCATTGACAAGGCACTGCCTGGCGCCCCACATGAGGTCCTGCTGGTACTGGATTCCAACACCGGGCAGAA
CGCGGTTACGCAGGTCAAAGCATTTGACGATGCCCTGGGCGTTACCGGCCTTGTGCTGAGCAAGCTGGATGGTACCGCCA
AGGGCGGCGTAATCGCGGCGATCGCACAATCGCGCCCCGTTCCCATCCGCTACATCGGCATCGGCGAATCCATCGACGAT
CTTCGTCCATTCAAGGCCAGCGAGTTCATCGATGCATTATTTGAATGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (56-117)

• SRP54-type protein, GTPase domain (SRP54) (135-334)

3D structure

• AlphaFold DB: Q1H3D6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	67.208	91.94	0.618