Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CUJ91_RS01935 Genome accession   NZ_CP024934
Coordinates   425730..427241 (-) Length   503 a.a.
NCBI ID   WP_006048026.1    Uniprot ID   B1FWW1
Organism   Paraburkholderia graminis strain PHS1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420730..432241
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUJ91_RS01910 (CUJ91_01905) - 421610..422245 (-) 636 WP_006048031.1 LON peptidase substrate-binding domain-containing protein -
  CUJ91_RS01915 (CUJ91_01910) rapZ 422285..423178 (-) 894 WP_006048030.1 RNase adapter RapZ -
  CUJ91_RS01920 (CUJ91_01915) hprK 423284..424252 (-) 969 WP_006048029.1 HPr(Ser) kinase/phosphatase -
  CUJ91_RS01925 (CUJ91_01920) - 424392..424907 (-) 516 WP_006048028.1 PTS sugar transporter subunit IIA -
  CUJ91_RS01930 (CUJ91_01925) raiA 425202..425558 (-) 357 WP_006048027.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CUJ91_RS01935 (CUJ91_01930) rpoN/rpoN1 425730..427241 (-) 1512 WP_006048026.1 RNA polymerase factor sigma-54 Machinery gene
  CUJ91_RS01940 (CUJ91_01935) lptB 427416..428201 (-) 786 WP_029969704.1 LPS export ABC transporter ATP-binding protein -
  CUJ91_RS01945 (CUJ91_01940) lptA 428198..428869 (-) 672 WP_006048024.1 lipopolysaccharide transport periplasmic protein LptA -
  CUJ91_RS01950 (CUJ91_01945) lptC 428891..429502 (-) 612 WP_006048023.1 LPS export ABC transporter periplasmic protein LptC -
  CUJ91_RS01955 (CUJ91_01950) - 429499..430059 (-) 561 WP_006048022.1 HAD family hydrolase -
  CUJ91_RS01960 (CUJ91_01955) kdsD 430059..431042 (-) 984 WP_029969706.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 503 a.a.        Molecular weight: 55311.35 Da        Isoelectric Point: 5.0028

>NTDB_id=256469 CUJ91_RS01935 WP_006048026.1 425730..427241(-) (rpoN/rpoN1) [Paraburkholderia graminis strain PHS1]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAISQNPLLENEDDWIASPLRVASDGSLITQAPSSSAPPEQM
GGNGSSSSSSERSENGEPQGVDEYNGLSGDGNGDASQWNLDDYGRSGNASDDDDLPPLQIHESSTSLRDHLMAQLRVTQA
GQRDRALVTFLIESLDDDGYLAATLEEVLADLPEELEVDLDEMNAALALLHSFDPAGVGARSASECLKLQLLRLEPSPTR
ALALDIVSHYLELLAARDFTRLRKHLKANDDDLRDAHMLIRSLEPFPGAAYGKAEADYVVPDIMVRKTAQGWQAELNPEV
VPKLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKSFFVHGEIAMRPLVLREIADT
LGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAENAKTPLSDSRIAELLAEQGFVV
ARRTVAKYREALRIPAVNLRKSL

Nucleotide


Download         Length: 1512 bp        

>NTDB_id=256469 CUJ91_RS01935 WP_006048026.1 425730..427241(-) (rpoN/rpoN1) [Paraburkholderia graminis strain PHS1]
ATGAAAGCCAGCCTCCAACTCCGCCTATCGCAGCATCTTGCGTTGACCCCGCAACTGCAGCAGTCCATCCGGCTGCTTCA
GCTGTCCACGCTCGAACTGCAGCAGGAAGTGGCAACGGCGATCTCGCAGAATCCGCTCCTCGAGAACGAGGACGACTGGA
TCGCGAGCCCGCTGCGCGTGGCGTCCGACGGCTCTCTGATCACGCAGGCGCCCAGTTCGTCCGCGCCGCCCGAGCAGATG
GGCGGCAATGGCTCGTCGTCGAGCAGCAGTGAGCGCTCGGAGAACGGCGAGCCGCAAGGCGTGGACGAATACAACGGCCT
CTCGGGCGACGGCAACGGCGACGCGTCGCAATGGAATCTCGACGACTACGGCCGCTCCGGCAACGCCTCCGACGACGACG
ATTTGCCGCCGCTGCAAATTCACGAATCGAGTACTTCGCTGCGCGATCACCTGATGGCGCAACTGCGCGTCACGCAGGCC
GGTCAGCGCGACCGCGCGCTCGTCACCTTCCTGATCGAGTCGCTCGACGACGACGGCTATCTTGCCGCCACGCTTGAAGA
AGTGCTCGCCGACTTGCCCGAAGAACTCGAAGTCGACCTGGACGAAATGAATGCGGCGCTCGCGCTGCTGCACAGTTTCG
ACCCCGCGGGCGTCGGCGCGCGTTCCGCGTCCGAATGCCTGAAGCTGCAATTGTTGCGGCTCGAACCCTCGCCTACGCGG
GCGCTTGCGCTCGACATCGTCAGCCATTATCTGGAACTGCTTGCGGCGCGCGATTTCACGCGTCTGCGCAAGCATCTGAA
GGCAAACGACGACGATCTGCGCGACGCGCACATGCTGATCCGCTCGCTCGAACCGTTTCCCGGCGCAGCGTATGGCAAGG
CCGAAGCGGATTACGTGGTGCCGGACATCATGGTGCGCAAAACCGCCCAGGGCTGGCAGGCCGAACTCAATCCCGAGGTC
GTGCCGAAGCTGCGCATCAACCACCTGTACGCCAACATTTTGCGCAATAACCGGGGCGATCCGGGCAGCGGTTCGTTGCG
CCAGCAACTGCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGTTTCGAGACGATCCTGCGGGTCGCGCAAGCCA
TTGTCGAGCGTCAAAAGAGCTTTTTTGTGCACGGCGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATTGCTGATACG
CTGGGCCTACACGAGTCGACTGTCTCGCGTGTGACAACCGGTAAGTACATGCTCACCCCGTTCGGGACGCTCGAGTTCAA
ATACTTCTTCGGTTCGCATGTTTCGACAGATACGGGCGGCGCGGCGTCTTCGACCGCCATTCGCGCGCTCATCAAGCAAC
TGATAGGAGCGGAAAACGCAAAAACGCCTCTCTCAGACAGCCGCATAGCCGAACTGCTGGCGGAACAGGGCTTCGTGGTC
GCACGGCGCACCGTGGCGAAATATCGCGAAGCGCTCAGAATCCCGGCAGTCAACCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(346-501)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(139-330)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

65.686

100

0.666

Multiple sequence alignment