Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   MFLA_RS00745 Genome accession   NC_007947
Coordinates   151989..153455 (+) Length   488 a.a.
NCBI ID   WP_011478510.1    Uniprot ID   Q1H524
Organism   Methylobacillus flagellatus KT     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 146989..158455
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MFLA_RS00715 (Mfla_0136) - 147168..148253 (-) 1086 WP_011478504.1 NAD(P)/FAD-dependent oxidoreductase -
  MFLA_RS00720 (Mfla_0137) - 148382..149380 (+) 999 WP_048811471.1 KpsF/GutQ family sugar-phosphate isomerase -
  MFLA_RS00725 (Mfla_0138) - 149420..149908 (+) 489 WP_195742038.1 KdsC family phosphatase -
  MFLA_RS00730 (Mfla_0139) lptC 149918..150607 (+) 690 WP_011478507.1 LPS export ABC transporter periplasmic protein LptC -
  MFLA_RS00735 (Mfla_0140) lptA 150633..151163 (+) 531 WP_011478508.1 lipopolysaccharide transport periplasmic protein LptA -
  MFLA_RS00740 (Mfla_0141) lptB 151247..151969 (+) 723 WP_011478509.1 LPS export ABC transporter ATP-binding protein -
  MFLA_RS00745 (Mfla_0142) rpoN/rpoN1 151989..153455 (+) 1467 WP_011478510.1 RNA polymerase factor sigma-54 Machinery gene
  MFLA_RS00750 (Mfla_0143) hpf 153486..153812 (+) 327 WP_011478511.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  MFLA_RS00755 (Mfla_0144) hprK 153912..154871 (+) 960 WP_011478512.1 HPr(Ser) kinase/phosphatase -
  MFLA_RS00760 (Mfla_0145) rapZ 154875..155717 (+) 843 WP_011478513.1 RNase adapter RapZ -
  MFLA_RS00765 (Mfla_0146) - 155764..156174 (+) 411 WP_011478514.1 PTS sugar transporter subunit IIA -
  MFLA_RS00770 (Mfla_0147) - 156167..156436 (+) 270 WP_011478515.1 HPr family phosphocarrier protein -
  MFLA_RS00775 (Mfla_0148) ptsP 156480..158219 (+) 1740 WP_011478516.1 phosphoenolpyruvate--protein phosphotransferase -

Sequence

Protein


Download         Length: 488 a.a.        Molecular weight: 54624.99 Da        Isoelectric Point: 4.9090

>NTDB_id=25646 MFLA_RS00745 WP_011478510.1 151989..153455(+) (rpoN/rpoN1) [Methylobacillus flagellatus KT]
MKQSLQLKFSQNLALTPQLQQSIKLLQLSSQELNQELESLLQANPLLERIDGPDGAAEISESYSPAPDAAVSAPASEAPA
PASEELPRIDEFGGGDDYYESFQGGSRWEEGGANSEDDNDFSFQEAANLTLREHLLSQIKLLPLSERDQTLAMVLVDAIN
DDGYLEQSLEELAELLPEELEIDPLELETALKHIQNLDPPGIGARNLAECLTLQLRALPAETPFVELAIKVAEQHLPLLA
SRDYTRLRKLLHCDDDVLKSVQQLVTQLNPRPGGEFSHITSDHYIQHEIVVKKIKGIWVASLNDEVVPKLRINQLYAGIL
KRNRDSSSQYLMSQMQEAKWMIKNIQQRFSTILRVSQAIVDRQRNFFEHGEVAMRPLVLREIADELGLHESTVSRVTTRK
HMLTPRGVYELKYFFGSHVATESGGACSATAIRALIKQMVSEENPKKPLSDNQITDILSKQGIVVARRTIAKYRESLQIP
AANQRKSL

Nucleotide


Download         Length: 1467 bp        

>NTDB_id=25646 MFLA_RS00745 WP_011478510.1 151989..153455(+) (rpoN/rpoN1) [Methylobacillus flagellatus KT]
ATGAAACAAAGTCTACAGCTAAAATTTTCCCAAAATCTCGCATTGACGCCGCAGCTGCAGCAGTCAATCAAATTGTTGCA
GCTGTCCTCGCAGGAACTCAACCAGGAGCTGGAGTCACTGTTGCAGGCCAATCCCTTGCTGGAGCGTATAGACGGTCCAG
ACGGTGCTGCCGAGATATCCGAAAGCTACAGTCCTGCCCCGGATGCTGCGGTTTCTGCTCCTGCCAGCGAAGCGCCTGCG
CCCGCGAGCGAGGAATTGCCACGCATTGACGAATTTGGCGGCGGGGATGATTACTATGAGTCTTTCCAAGGAGGTAGCCG
CTGGGAAGAAGGCGGCGCTAATAGTGAAGACGATAACGACTTCAGCTTTCAGGAAGCGGCCAACCTCACTCTGCGTGAAC
ACCTGCTGAGCCAGATCAAGCTGCTGCCTTTGTCGGAACGTGATCAGACCTTGGCGATGGTACTGGTAGATGCGATCAAT
GACGATGGGTATCTGGAACAGTCCCTGGAAGAGCTGGCCGAGCTTTTGCCGGAAGAGCTGGAGATCGATCCGCTCGAGCT
TGAAACGGCACTCAAGCATATCCAGAATCTGGATCCGCCGGGGATTGGCGCCCGCAACCTGGCAGAATGCCTGACATTGC
AACTGCGTGCCTTGCCGGCAGAAACACCTTTTGTGGAGTTGGCGATCAAGGTTGCCGAACAGCATCTGCCCCTGCTGGCA
AGTCGTGATTACACGCGCTTGCGTAAACTGCTGCATTGCGATGATGATGTCCTGAAATCGGTGCAGCAACTGGTGACCCA
GCTCAATCCGCGACCAGGCGGCGAATTCAGCCACATTACTTCGGATCATTACATCCAGCATGAAATCGTGGTGAAGAAGA
TCAAGGGGATCTGGGTTGCCAGCCTGAATGATGAGGTCGTACCCAAGCTGCGTATCAACCAGCTTTATGCCGGCATCCTG
AAACGGAATCGTGACAGCTCCAGTCAATATCTCATGAGTCAGATGCAGGAGGCGAAATGGATGATCAAGAATATCCAGCA
GCGCTTCTCCACTATCCTGCGTGTGTCGCAGGCAATCGTGGACCGGCAGCGTAATTTCTTCGAGCACGGCGAAGTGGCCA
TGCGCCCGTTGGTGCTTCGGGAGATTGCCGATGAGCTGGGCCTGCATGAATCGACCGTGTCGCGCGTGACCACAAGAAAG
CACATGCTCACACCGCGCGGTGTTTACGAGTTGAAGTATTTCTTCGGCAGCCATGTGGCGACAGAGTCGGGAGGAGCGTG
CTCCGCCACGGCGATCCGTGCGTTGATCAAGCAGATGGTGAGCGAGGAAAACCCGAAGAAGCCGCTGTCCGACAATCAGA
TCACCGATATCCTGAGCAAGCAAGGGATTGTCGTGGCAAGAAGAACTATAGCGAAATACCGCGAGTCTCTACAGATACCC
GCGGCCAACCAGCGCAAGTCGCTTTGA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(329-486)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(126-316)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q1H524

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

52.259

100

0.545

Multiple sequence alignment