Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CUN65_RS02275 Genome accession   NZ_CP024908
Coordinates   476904..478337 (-) Length   477 a.a.
NCBI ID   WP_003861864.1    Uniprot ID   -
Organism   Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471904..483337
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUN65_RS02245 (CUN65_02275) elbB 473436..474089 (+) 654 WP_023314910.1 isoprenoid biosynthesis glyoxalase ElbB -
  CUN65_RS02250 (CUN65_02280) mtgA 474086..474811 (+) 726 WP_003861874.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CUN65_RS02255 (CUN65_02285) npr 474851..475123 (-) 273 WP_003861872.1 PTS phosphocarrier protein NPr -
  CUN65_RS02260 (CUN65_02290) rapZ 475120..475974 (-) 855 WP_003861870.1 RNase adapter RapZ -
  CUN65_RS02265 (CUN65_02295) ptsN 476020..476511 (-) 492 WP_003861869.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CUN65_RS02270 (CUN65_02300) hpf 476594..476881 (-) 288 WP_003861866.1 ribosome hibernation promoting factor -
  CUN65_RS02275 (CUN65_02305) rpoN/rpoN1 476904..478337 (-) 1434 WP_003861864.1 RNA polymerase factor sigma-54 Machinery gene
  CUN65_RS02280 (CUN65_02310) lptB 478385..479110 (-) 726 WP_003861862.1 LPS export ABC transporter ATP-binding protein -
  CUN65_RS02285 (CUN65_02315) lptA 479117..479671 (-) 555 WP_003861860.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  CUN65_RS02290 (CUN65_02320) lptC 479640..480215 (-) 576 WP_003861858.1 LPS export ABC transporter periplasmic protein LptC -
  CUN65_RS02295 (CUN65_02325) kdsC 480212..480778 (-) 567 WP_003861856.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CUN65_RS02300 (CUN65_02330) kdsD 480793..481779 (-) 987 WP_003861853.1 arabinose-5-phosphate isomerase KdsD -
  CUN65_RS02305 (CUN65_02335) - 481793..482770 (-) 978 WP_003861852.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54099.96 Da        Isoelectric Point: 4.3358

>NTDB_id=256372 CUN65_RS02275 WP_003861864.1 476904..478337(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDAQQTQDTETLDSVDALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDE
ILESIGDDEIELEEIEAVLKRVQRFDPIGVAAKDLRDCLLIQLSQFAKETPWIDEARLIISDHLDLLANHDFRTLMRVTR
LKEEVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNADSIPRLQINQQYASMCTSARNDADNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=256372 CUN65_RS02275 WP_003861864.1 476904..478337(-) (rpoN/rpoN1) [Enterobacter hormaechei subsp. xiangfangensis strain OSUKPC4_L]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAGCAACTGGCCATGACGCCGCAGCTCCAGCAGGCAATCCGTCTGTTGCA
GCTGTCCACGTTAGAACTCCAGCAGGAGCTCCAGCAGGCGCTGGACAGCAATCCGCTGCTGGAGCAGACCGATCTTCATG
ACGAGGTAGACGCCCAGCAGACGCAGGACACAGAAACGCTCGACTCCGTAGATGCACTCGAACAGAAAGAGATGCCGGAC
GAACTGCCGCTGGATGCCAGCTGGGATGAAATCTACACCGCGGGTACCCCTTCCGGGACGCGTGCAGACTACCAGGACGA
TGAACTCCCGGTCTACCAGGGCGAAACTACCCAATCGCTTCAGGACTACCTGATGTGGCAGGTGGAGCTGACGCCTTTCT
CCGATACCGATCGCGCCATTGCCACCTCGATTGTCGATGCCGTTGACGACACGGGCTATTTGACCGTGACGCTGGACGAG
ATTCTTGAAAGCATCGGCGATGACGAGATTGAACTGGAAGAGATCGAAGCCGTCCTGAAACGCGTTCAGCGTTTCGACCC
GATTGGCGTCGCCGCGAAAGATCTGCGTGACTGCCTTCTGATCCAGCTTTCGCAGTTTGCTAAAGAGACACCGTGGATTG
ACGAAGCCCGGCTCATCATCAGCGATCATCTGGATCTGCTGGCGAACCACGACTTCCGCACCCTGATGCGCGTCACGCGC
CTGAAAGAAGAGGTGCTGAAGGAAGCGGTGAATCTGATCCAGTCGCTCGATCCGCGTCCGGGGCAGTCGATTCAGACCAG
CGAACCGGAATATGTCATTCCCGATGTGCTGGTGAGAAAACACAATGGCCGCTGGGTCGTTGAACTGAACGCCGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAATACGCCTCCATGTGCACCAGCGCCCGTAACGACGCTGATAATCAATACATTCGC
AGCAACCTTCAGGAAGCACGCTGGTTGATTAAGAGTCTGGAGAGCCGTAATGACACGCTGCTGCGCGTCAGCCGCTGTAT
TGTCGAACAGCAGCAGGCATTCTTTGAGCAGGGCGAAGAGTTTATGAAACCGATGGTGCTGGCGGACATAGCCCAGGCCG
TCGAGATGCATGAATCAACCATTTCCCGCGTGACCACGCAGAAGTATCTGCACAGCCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTGAACACCGAAGGCGGCGGCGAAGCCTCGTCGACGGCCATTCGCGCACTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTCAGTGACAGTAAGTTAACCACCATGCTGTCCGATCAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment