Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CUB85_RS12960 Genome accession   NZ_CP024797
Coordinates   2622601..2623368 (-) Length   255 a.a.
NCBI ID   WP_015240232.1    Uniprot ID   -
Organism   Bacillus velezensis strain TJ02     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2617601..2628368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS12940 - 2617803..2618615 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  CUB85_RS12945 ccpN 2618641..2619279 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  CUB85_RS12950 glyS 2619380..2621419 (-) 2040 WP_047935962.1 glycine--tRNA ligase subunit beta -
  CUB85_RS12955 glyQ 2621412..2622299 (-) 888 WP_094031841.1 glycine--tRNA ligase subunit alpha -
  CUB85_RS12960 recO 2622601..2623368 (-) 768 WP_015240232.1 DNA repair protein RecO Machinery gene
  CUB85_RS12965 - 2623405..2623548 (-) 144 WP_007408286.1 YqzL family protein -
  CUB85_RS12970 era 2623703..2624608 (-) 906 WP_094031842.1 GTPase Era -
  CUB85_RS12975 - 2624589..2624999 (-) 411 WP_003152976.1 cytidine deaminase -
  CUB85_RS12980 - 2625123..2625497 (-) 375 WP_015240233.1 diacylglycerol kinase family protein -
  CUB85_RS12985 ybeY 2625478..2625951 (-) 474 WP_012118016.1 rRNA maturation RNase YbeY -
  CUB85_RS12990 - 2625952..2628084 (-) 2133 WP_094031843.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29294.89 Da        Isoelectric Point: 8.7092

>NTDB_id=255508 CUB85_RS12960 WP_015240232.1 2622601..2623368(-) (recO) [Bacillus velezensis strain TJ02]
MLTKSEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=255508 CUB85_RS12960 WP_015240232.1 2622601..2623368(-) (recO) [Bacillus velezensis strain TJ02]
ATGCTGACTAAAAGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.235

100

0.882

Multiple sequence alignment