Detailed information    

insolico Bioinformatically predicted

Overview

Name   spo0A   Type   Regulator
Locus tag   CUB85_RS12455 Genome accession   NZ_CP024797
Coordinates   2537315..2538115 (-) Length   266 a.a.
NCBI ID   WP_003153177.1    Uniprot ID   I2C7K5
Organism   Bacillus velezensis strain TJ02     
Function   activation and repression of comK; repression of rok; repression of abrB (predicted from homology)   
Competence regulation

Genomic Context

Location: 2532315..2543115
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS12425 - 2532861..2533493 (+) 633 WP_007408365.1 helix-turn-helix domain-containing protein -
  CUB85_RS20655 - 2533784..2533921 (+) 138 WP_014470646.1 hypothetical protein -
  CUB85_RS12430 - 2534015..2535133 (+) 1119 WP_039063421.1 NADH-dependent flavin oxidoreductase -
  CUB85_RS12435 - 2535260..2535472 (+) 213 WP_039063422.1 spore germination protein -
  CUB85_RS12440 - 2535597..2535809 (+) 213 WP_003153181.1 spore germination protein -
  CUB85_RS12445 alr 2535985..2537166 (+) 1182 WP_039063308.1 alanine racemase -
  CUB85_RS12455 spo0A 2537315..2538115 (-) 801 WP_003153177.1 sporulation transcription factor Spo0A Regulator
  CUB85_RS12460 spoIVB 2538396..2539676 (-) 1281 WP_039063309.1 SpoIVB peptidase -
  CUB85_RS12465 recN 2539840..2541570 (-) 1731 WP_032874060.1 DNA repair protein RecN Machinery gene
  CUB85_RS12470 ahrC 2541602..2542051 (-) 450 WP_003153173.1 transcriptional regulator AhrC/ArgR -
  CUB85_RS12475 - 2542148..2542993 (-) 846 WP_007612503.1 TlyA family RNA methyltransferase -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 29630.11 Da        Isoelectric Point: 6.1630

>NTDB_id=255500 CUB85_RS12455 WP_003153177.1 2537315..2538115(-) (spo0A) [Bacillus velezensis strain TJ02]
MEKIKVCVADDNRELVSLLSEYIEGQDDMEVIGVAYNGQECLSLFKDKNPDVLVLDIIMPHLDGLAVLERLRESELEKQP
NVIMLTAFGQEDVTKKAVDLGASYFILKPFDMENLVGHIRQVSGNVSSVTHRAPSAQSSIIRSQPEPKRKNLDASITSII
HEIGVPAHIKGYLYLREAISMVYNDIELLGSITKVLYPDIAKKFNTTASRVERAIRHAIEVAWSRGNIDSISSLFGYTVS
MTKAKPTNSEFIAMVADKLRLEHKAS

Nucleotide


Download         Length: 801 bp        

>NTDB_id=255500 CUB85_RS12455 WP_003153177.1 2537315..2538115(-) (spo0A) [Bacillus velezensis strain TJ02]
GTGGAGAAAATTAAAGTTTGTGTTGCTGATGATAATCGAGAGCTTGTAAGCCTGTTGAGTGAATATATAGAGGGACAGGA
TGATATGGAAGTGATCGGCGTTGCTTATAACGGACAGGAATGTCTTTCTTTGTTTAAAGATAAGAATCCAGATGTGCTCG
TTTTAGACATTATCATGCCGCATCTGGACGGACTTGCGGTTTTGGAGCGTCTGCGGGAATCAGAGCTCGAGAAGCAGCCG
AACGTCATCATGCTGACGGCATTCGGTCAGGAAGACGTTACGAAAAAAGCCGTCGATTTAGGCGCGTCCTATTTCATCCT
GAAGCCGTTTGATATGGAAAACCTTGTCGGCCATATTCGCCAGGTAAGCGGAAATGTCAGCAGTGTCACACACCGTGCGC
CGTCTGCGCAAAGCAGCATTATCCGCAGCCAGCCTGAGCCGAAGCGGAAAAATCTCGATGCCAGCATTACAAGCATTATC
CATGAGATCGGTGTGCCTGCCCATATCAAAGGCTATCTTTATTTGCGTGAAGCCATTTCCATGGTGTATAACGATATTGA
ACTCCTCGGCAGCATCACAAAAGTCCTTTATCCCGACATCGCCAAAAAATTCAACACGACCGCAAGCCGTGTAGAACGGG
CCATCCGCCACGCCATTGAAGTCGCATGGAGCAGGGGAAATATTGATTCCATTTCTTCACTGTTCGGATATACGGTAAGC
ATGACAAAAGCAAAGCCAACCAATTCAGAGTTCATCGCGATGGTTGCTGACAAGCTGAGATTGGAGCATAAAGCTTCGTA
A

Domains

Predicted by InterproScan.

Sporulation initiation factor Spo0A C terminal (Spo0A_C)

(156-259)

Response regulator receiver domain (Response_reg)

(6-119)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I2C7K5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  spo0A Bacillus subtilis subsp. subtilis str. 168

96.629

100

0.97

Multiple sequence alignment