Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CUB85_RS09375 Genome accession   NZ_CP024797
Coordinates   1873999..1874433 (+) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain TJ02     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1868999..1879433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS09370 - 1872499..1873710 (-) 1212 WP_007409852.1 cytochrome P450 family protein -
  CUB85_RS09375 nucA/comI 1873999..1874433 (+) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CUB85_RS09380 - 1874493..1875248 (+) 756 WP_072589115.1 YoaK family protein -
  CUB85_RS09385 - 1875282..1875644 (-) 363 WP_007410383.1 hypothetical protein -
  CUB85_RS09390 - 1875838..1877166 (-) 1329 WP_015239896.1 S8 family peptidase -
  CUB85_RS09395 - 1877345..1877578 (+) 234 WP_015239897.1 hypothetical protein -
  CUB85_RS09400 - 1877834..1878541 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  CUB85_RS09405 - 1878601..1879053 (+) 453 WP_039251441.1 OsmC family protein -
  CUB85_RS09410 - 1879067..1879420 (-) 354 WP_094031727.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=255498 CUB85_RS09375 WP_007611576.1 1873999..1874433(+) (nucA/comI) [Bacillus velezensis strain TJ02]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=255498 CUB85_RS09375 WP_007611576.1 1873999..1874433(+) (nucA/comI) [Bacillus velezensis strain TJ02]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGAGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment