Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CUB85_RS02195 Genome accession   NZ_CP024797
Coordinates   388421..388858 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain TJ02     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 383421..393858
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUB85_RS02170 - 384440..385576 (+) 1137 WP_094031490.1 zinc-dependent alcohol dehydrogenase -
  CUB85_RS02175 - 385591..386025 (+) 435 WP_094031491.1 RDD family protein -
  CUB85_RS02180 - 386143..386421 (+) 279 WP_263612330.1 YckD family protein -
  CUB85_RS02185 - 386525..387961 (+) 1437 WP_076425807.1 glycoside hydrolase family 1 protein -
  CUB85_RS02190 nin/comJ 388002..388400 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  CUB85_RS02195 nucA/comI 388421..388858 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CUB85_RS02200 hxlB 389217..389774 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  CUB85_RS02205 hxlA 389771..390406 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  CUB85_RS02210 - 390638..391000 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=255470 CUB85_RS02195 WP_003156588.1 388421..388858(-) (nucA/comI) [Bacillus velezensis strain TJ02]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=255470 CUB85_RS02195 WP_003156588.1 388421..388858(-) (nucA/comI) [Bacillus velezensis strain TJ02]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment