Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CUC43_RS09440 Genome accession   NZ_CP024771
Coordinates   1720794..1722170 (+) Length   458 a.a.
NCBI ID   WP_029439115.1    Uniprot ID   -
Organism   Bacillus thuringiensis LM1212     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1715794..1727170
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC43_RS09420 (CUC43_09720) ctsR 1715990..1716451 (+) 462 WP_001244563.1 transcriptional regulator CtsR -
  CUC43_RS09425 (CUC43_09725) - 1716625..1717170 (+) 546 WP_029439113.1 UvrB/UvrC motif-containing protein -
  CUC43_RS09430 (CUC43_09730) - 1717175..1718239 (+) 1065 WP_000050825.1 protein arginine kinase -
  CUC43_RS09435 (CUC43_09735) clpC 1718262..1720697 (+) 2436 WP_029439114.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  CUC43_RS09440 (CUC43_09740) radA 1720794..1722170 (+) 1377 WP_029439115.1 DNA repair protein RadA Machinery gene
  CUC43_RS09445 (CUC43_09745) disA 1722174..1723247 (+) 1074 WP_000392166.1 DNA integrity scanning diadenylate cyclase DisA -
  CUC43_RS09450 (CUC43_09750) - 1723408..1724517 (+) 1110 WP_000919684.1 PIN/TRAM domain-containing protein -
  CUC43_RS09455 (CUC43_09755) ispD 1724534..1725214 (+) 681 WP_029439116.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CUC43_RS09460 (CUC43_09760) ispF 1725330..1725806 (+) 477 WP_000488386.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49836.49 Da        Isoelectric Point: 7.0904

>NTDB_id=255364 CUC43_RS09440 WP_029439115.1 1720794..1722170(+) (radA) [Bacillus thuringiensis LM1212]
MAKKKTKFTCQECGYQSPKYMGKCPGCGQWNTLVEEMEPVVSSRRLNYANAIQSEVTKPRRLTEVETKSEARIETKFQEF
NRVLGGGIVDGSLVLIGGDPGIGKSTLLLQISSQLADASYDVLYISGEESAKQIKLRADRLDVKGSNLFVVAETDLQRIA
THIEEMNPAFVVIDSIQTIHLPEVTSAPGSVAQVRECTAELMKLAKTKGIPIFIVGHVTKEGAIAGPRMLEHMVDAVLYF
EGDRHHTYRILRAVKNRFGSTNEMGIFEMKELGLAEVLNPSEIFLEERPVGVAGSTVVASMEGTRPVLVEIQALISPTSF
GNPRRMATGIDHNRVSLIMAVLEKRTGLLLQNQDAYLKVAGGLKLDEPAIDLAVALSIASSFRDKSTAPTDAVIGEVGLT
GEIRRVSRIEQRVQEAAKLGFQRAIIPRKNLGGWTIPDGIEVVGVSNLGEALRLTLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=255364 CUC43_RS09440 WP_029439115.1 1720794..1722170(+) (radA) [Bacillus thuringiensis LM1212]
ATGGCTAAAAAGAAAACAAAATTTACATGTCAAGAGTGTGGTTATCAGTCACCAAAATATATGGGTAAATGCCCTGGATG
TGGTCAATGGAATACGCTCGTTGAAGAGATGGAGCCGGTTGTATCATCAAGGCGCCTTAATTATGCTAATGCAATTCAAT
CAGAAGTAACAAAACCAAGACGCTTAACAGAAGTAGAAACAAAATCTGAAGCGCGTATTGAAACAAAATTTCAGGAATTT
AACCGTGTACTAGGTGGTGGCATTGTAGATGGATCTTTAGTACTTATTGGTGGGGATCCTGGGATTGGAAAATCAACGTT
ATTATTACAAATTTCCTCGCAATTAGCAGATGCTTCATACGATGTATTGTACATATCAGGTGAGGAGTCAGCAAAGCAGA
TAAAACTTCGTGCAGATCGTTTGGATGTAAAGGGTAGTAATCTATTTGTTGTAGCAGAGACTGACTTACAGCGAATTGCA
ACACACATTGAGGAAATGAATCCAGCCTTTGTTGTTATTGATTCTATTCAAACGATACATTTACCTGAGGTGACGTCAGC
GCCGGGAAGTGTGGCACAAGTACGTGAATGTACAGCAGAATTAATGAAACTTGCAAAAACGAAAGGAATCCCTATTTTTA
TAGTTGGGCATGTGACGAAAGAGGGAGCGATTGCAGGGCCGCGTATGTTAGAACATATGGTCGATGCAGTTCTTTACTTT
GAAGGAGACCGTCATCATACATACCGTATTTTGCGAGCTGTGAAAAATCGTTTTGGTTCTACGAATGAAATGGGTATTTT
TGAAATGAAAGAACTTGGTCTTGCAGAAGTCTTAAACCCTTCTGAAATTTTCCTTGAGGAAAGACCAGTTGGGGTTGCAG
GATCAACAGTGGTTGCCTCAATGGAAGGAACAAGGCCAGTTTTAGTAGAAATACAAGCATTAATCTCCCCTACTAGTTTT
GGAAACCCTCGAAGAATGGCAACGGGAATTGATCATAACCGTGTATCGCTTATTATGGCGGTGTTAGAAAAAAGAACAGG
CTTATTATTGCAAAATCAGGACGCATATTTAAAAGTAGCAGGTGGTTTGAAGTTAGATGAACCAGCAATTGATTTAGCTG
TTGCTTTAAGTATAGCTTCAAGTTTTAGAGATAAATCTACGGCGCCAACCGATGCAGTAATAGGAGAAGTGGGATTAACT
GGAGAAATAAGAAGAGTATCAAGAATTGAACAACGTGTACAAGAAGCAGCTAAATTAGGATTTCAACGCGCTATTATTCC
TAGAAAAAATTTGGGGGGATGGACAATTCCGGATGGGATTGAGGTAGTTGGTGTATCTAATTTAGGAGAAGCGCTTCGTT
TGACATTAGGAGGCTAG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(363-430)

AAA domain (AAA_25)

(60-222)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

74.292

100

0.745
  radA Streptococcus mitis NCTC 12261

64.018

98.908

0.633
  radA Streptococcus pneumoniae Rx1

63.797

98.908

0.631
  radA Streptococcus pneumoniae D39

63.797

98.908

0.631
  radA Streptococcus pneumoniae R6

63.797

98.908

0.631
  radA Streptococcus pneumoniae TIGR4

63.797

98.908

0.631
  radA Streptococcus mitis SK321

63.797

98.908

0.631

Multiple sequence alignment