Detailed information NTG0255360

Bioinformatically predicted

Overview

• Name: recF
• Type: Machinery gene
• Locus tag: CUC43_RS08930
• Genome accession: NZ_CP024771
• Coordinates: 1626244..1627371 (+)
• Length: 375 a.a.
• NCBI ID: WP_000470753.1
• Uniprot ID: Q6I535
• Organism: Bacillus thuringiensis LM1212
• Function: homologous recombination (predicted from homology) Homologous recombination

Genomic Context

Location: 1621244..1632371

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUC43_RS08900 (CUC43_09190)	spoIIIJ	1621289..1622056 (-)	768	WP_029440295.1	YidC family membrane integrase SpoIIIJ	-
CUC43_RS08905 (CUC43_09195)	rnpA	1622109..1622456 (-)	348	WP_029440294.1	ribonuclease P protein component	-
CUC43_RS08910 (CUC43_09200)	rpmH	1622533..1622667 (-)	135	WP_000831901.1	50S ribosomal protein L34	-
CUC43_RS08915 (CUC43_09205)	dnaA	1623233..1624573 (+)	1341	WP_000428025.1	chromosomal replication initiator protein DnaA	-
CUC43_RS08920 (CUC43_09210)	dnaN	1624752..1625891 (+)	1140	WP_001212891.1	DNA polymerase III subunit beta	-
CUC43_RS08925 (CUC43_09215)	yaaA	1626019..1626231 (+)	213	WP_000821367.1	S4 domain-containing protein YaaA	-
CUC43_RS08930 (CUC43_09220)	recF	1626244..1627371 (+)	1128	WP_000470753.1	DNA replication/repair protein RecF	Machinery gene
CUC43_RS08935 (CUC43_09225)	gyrB	1627410..1629332 (+)	1923	WP_033694820.1	DNA topoisomerase (ATP-hydrolyzing) subunit B	-
CUC43_RS08940 (CUC43_09230)	gyrA	1629421..1631892 (+)	2472	WP_029440292.1	DNA gyrase subunit A	-

Sequence

Protein

• Length: 375 a.a.
• Molecular weight: 43362.71 Da
• Isoelectric Point: 6.9850

>NTDB_id=255360 CUC43_RS08930 WP_000470753.1 1626244..1627371(+) (recF) [Bacillus thuringiensis LM1212]
MFISEIQLKNYRNYEKLELSFEDKVNVIIGENAQGKTNLMEAIYVLAMAKSHRTSNDRELIRWDEDFGQIKGKLQKRNSS
LSLELNISKKGKKAKLNQLEQQKLSQYIGVMNVVMFAPEDLNLVKGSPQVRRRFLDMELGQIAPVYLYELSQYQKVLTQR
NHLLKKMQGNSKNEETMLDVFTLQLIEHGTKILRKRFEFLHLLQEWAAPIHRGISRGLEELEIVYKPSVDVSESMDLSKI
KEVYYESFQSVKQREIFRGTTLIGPHRDDLQFFVNSKNVQVFGSQGQQRTTALSLKLAEIELIYSEVKEYPILLLDDVLS
ELDDYRQSHLLNTIQGKVQTFVTTTSVDGIEHETLKEAKTIHVTNGTVDCEIDRA

Nucleotide

• Length: 1128 bp

>NTDB_id=255360 CUC43_RS08930 WP_000470753.1 1626244..1627371(+) (recF) [Bacillus thuringiensis LM1212]
TTGTTTATTTCAGAAATACAATTAAAAAACTATCGCAATTATGAAAAATTAGAGCTTTCCTTTGAAGATAAGGTAAATGT
AATTATCGGCGAAAATGCGCAAGGGAAAACGAATTTGATGGAAGCTATTTATGTTTTAGCGATGGCAAAATCTCATAGAA
CCTCTAATGATCGTGAGCTTATCCGTTGGGATGAAGATTTCGGTCAAATTAAAGGGAAGTTACAAAAGAGAAATAGTTCT
TTGTCTTTGGAATTAAATATCTCTAAAAAAGGTAAAAAGGCAAAATTAAATCAACTTGAACAACAAAAATTGAGTCAATA
TATTGGCGTGATGAATGTTGTCATGTTTGCCCCAGAAGATTTAAATCTTGTAAAAGGAAGCCCTCAAGTAAGAAGACGCT
TTTTAGATATGGAATTAGGCCAAATAGCTCCGGTCTATTTGTACGAATTGAGTCAATATCAAAAGGTGCTCACACAACGA
AATCACTTGCTGAAAAAGATGCAAGGGAATAGTAAGAATGAGGAAACGATGTTGGATGTATTTACACTTCAACTTATTGA
GCATGGTACAAAAATATTGCGAAAACGCTTTGAATTTTTGCATTTACTACAAGAATGGGCAGCTCCAATTCATCGTGGTA
TAAGCCGTGGATTAGAAGAATTAGAAATTGTCTATAAACCGAGTGTAGATGTATCAGAATCAATGGATTTGTCGAAAATA
AAAGAAGTATACTATGAAAGTTTTCAATCAGTGAAACAACGTGAAATTTTCCGTGGTACGACTTTAATTGGTCCTCATCG
TGATGATTTACAATTCTTCGTTAATAGTAAAAATGTTCAAGTCTTTGGTTCGCAAGGACAACAACGAACGACCGCACTGT
CCCTAAAATTAGCTGAAATTGAATTGATTTACTCAGAAGTTAAGGAATATCCGATTCTTTTATTGGATGATGTATTATCA
GAATTAGATGATTATCGTCAATCGCATCTGTTAAATACAATTCAAGGAAAAGTGCAAACATTTGTGACAACGACGAGTGT
CGACGGAATTGAACACGAAACATTAAAAGAAGCGAAAACAATTCATGTAACGAACGGCACGGTAGATTGTGAAATAGATA
GGGCGTAA

Domains

Predicted by InterproScan.

• RecF/RecN/SMC N terminal domain (SMC_N) (2-348)

3D structure

• AlphaFold DB: Q6I535

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
recF	Bacillus subtilis subsp. subtilis str. 168	66.304	98.133	0.651