Detailed information NTG0255354

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: CUC43_RS07370
• Genome accession: NZ_CP024771
• Coordinates: 1297760..1299109 (-)
• Length: 449 a.a.
• NCBI ID: WP_029440992.1
• Uniprot ID: -
• Organism: Bacillus thuringiensis LM1212
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1292760..1304109

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CUC43_RS07350 (CUC43_07600)	secA	1293083..1295590 (-)	2508	WP_029440990.1	preprotein translocase subunit SecA	-
CUC43_RS07355 (CUC43_07605)	raiA	1295866..1296408 (-)	543	WP_000671191.1	ribosome-associated translation inhibitor RaiA	-
CUC43_RS07360 (CUC43_07610)	cspC	1296732..1296929 (-)	198	WP_001990088.1	cold shock protein CspC	-
CUC43_RS07365 (CUC43_07615)	-	1297056..1297760 (-)	705	WP_029440991.1	ComF family protein	-
CUC43_RS07370 (CUC43_07620)	comFA	1297760..1299109 (-)	1350	WP_029440992.1	ATP-dependent helicase ComFA	Machinery gene
CUC43_RS07375 (CUC43_07625)	-	1299237..1300625 (-)	1389	WP_029440993.1	C40 family peptidase	-
CUC43_RS07380 (CUC43_07630)	-	1300774..1301088 (-)	315	WP_029440994.1	helix-turn-helix domain-containing protein	-
CUC43_RS07385 (CUC43_07640)	-	1301262..1302104 (-)	843	WP_029440995.1	DegV family protein	-
CUC43_RS07390 (CUC43_07645)	-	1302344..1302979 (+)	636	WP_000926680.1	YigZ family protein	-

Sequence

Protein

• Length: 449 a.a.
• Molecular weight: 51123.78 Da
• Isoelectric Point: 9.5958

>NTDB_id=255354 CUC43_RS07370 WP_029440992.1 1297760..1299109(-) (comFA) [Bacillus thuringiensis LM1212]
MLAGKQLLLEEISSDLRRELSDLKEKGEVICVQGVIKKASKYICQRCGNIEQRLFASFLCKRCGKACMYCRKCITMGRVS
ECTVLVRGINERKRERELNSLQWKGALATGQELAAQGVIEAIKQKESFFIWAVCGAGKTEMLFYGIEEALQKGERVCIAT
PRTDVVMELAPRLQEVFPSINVAALYGGSVDHEKDAALVVATTHQLLRYYRAFHVMIVDEIDAFPYHADQMLQYAVQQAM
KEKAARIYLTATPDEKWKRNFRMGKQKGIIVSGRYHRHPLPVPLFSWCGNWKKSLHHKKIPRVLLQWLKMNLNKKYPIFL
FVPHVRYIEDVSLLLKGLDNRIDGVHAEDPMRKEKVTSFRKGDIPLLVTTTILERGVTVKNLQVAVLGAEEEIFSESALV
QIAGRAGRSFEEPYGEVVYFHYGKTEAMVRAKKHIQSMNKSAKEQGLID

Nucleotide

• Length: 1350 bp

>NTDB_id=255354 CUC43_RS07370 WP_029440992.1 1297760..1299109(-) (comFA) [Bacillus thuringiensis LM1212]
ATGCTTGCTGGAAAGCAGTTGCTATTAGAAGAAATATCTTCAGACTTAAGGAGAGAATTGAGTGATTTAAAAGAGAAGGG
AGAGGTCATATGTGTACAAGGCGTAATAAAGAAGGCTTCTAAATATATATGTCAGCGCTGCGGAAATATAGAGCAGCGGC
TATTTGCATCATTTTTATGTAAAAGATGCGGTAAAGCATGCATGTATTGCCGGAAGTGTATAACGATGGGGAGAGTTAGT
GAATGTACTGTACTTGTTCGAGGGATTAATGAAAGAAAAAGAGAAAGGGAATTAAATTCGTTACAGTGGAAAGGTGCATT
GGCTACTGGTCAGGAGTTGGCGGCGCAAGGTGTTATAGAAGCGATTAAGCAGAAAGAATCTTTTTTTATTTGGGCTGTGT
GCGGGGCTGGAAAAACAGAAATGTTATTTTACGGTATAGAAGAGGCGCTTCAAAAAGGAGAAAGAGTTTGTATCGCAACA
CCAAGGACGGACGTTGTAATGGAATTAGCTCCAAGATTACAAGAAGTGTTTCCAAGTATAAATGTAGCTGCTTTATACGG
AGGGAGTGTAGATCATGAAAAAGATGCAGCGTTAGTCGTTGCAACGACTCACCAATTGTTACGTTACTATAGAGCGTTTC
ATGTGATGATTGTAGATGAGATTGATGCCTTCCCGTATCATGCGGATCAAATGTTACAGTATGCAGTGCAACAAGCGATG
AAAGAGAAAGCAGCGCGTATTTATTTAACTGCAACCCCTGATGAAAAGTGGAAGCGCAATTTCAGAATGGGGAAACAAAA
AGGTATCATTGTTTCGGGACGATATCATCGTCATCCGTTACCAGTTCCTCTATTTAGTTGGTGCGGAAATTGGAAGAAAA
GCCTCCATCATAAAAAAATTCCTCGCGTGTTACTACAATGGTTAAAAATGAACTTAAACAAAAAGTATCCTATTTTTTTA
TTTGTTCCTCATGTGCGATATATAGAAGATGTGAGCCTGTTATTGAAAGGATTGGATAATCGAATCGATGGCGTACATGC
AGAAGATCCGATGAGAAAAGAAAAAGTGACATCCTTCAGAAAGGGAGACATTCCGTTATTAGTTACAACGACAATTTTAG
AAAGAGGAGTAACTGTGAAGAACTTACAAGTGGCTGTGTTAGGAGCTGAAGAAGAAATATTTTCGGAAAGTGCGCTCGTA
CAAATTGCAGGCCGAGCAGGTCGTAGTTTTGAAGAACCTTATGGTGAGGTTGTTTATTTTCATTACGGTAAGACAGAGGC
GATGGTACGTGCGAAAAAACATATTCAAAGTATGAACAAAAGTGCGAAAGAACAAGGATTGATCGATTAA

Domains

Predicted by InterproScan.

• Helicase conserved C-terminal domain (Helicase_C) (311-408)

• Type III restriction enzyme, res subunit (ResIII) (107-254)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Bacillus subtilis subsp. subtilis str. 168	50.922	96.659	0.492
comFA	Latilactobacillus sakei subsp. sakei 23K	40.741	90.2	0.367