Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CUC43_RS02550 Genome accession   NZ_CP024771
Coordinates   439959..440705 (-) Length   248 a.a.
NCBI ID   WP_000487010.1    Uniprot ID   Q81LT9
Organism   Bacillus thuringiensis LM1212     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 434959..445705
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC43_RS02535 (CUC43_02625) dnaG 435728..437524 (-) 1797 WP_029439944.1 DNA primase -
  CUC43_RS02540 (CUC43_02645) - 438377..439189 (-) 813 WP_000368943.1 pyruvate, water dikinase regulatory protein -
  CUC43_RS02545 (CUC43_02650) - 439219..439851 (-) 633 WP_000583759.1 helix-turn-helix transcriptional regulator -
  CUC43_RS02550 (CUC43_02655) recO 439959..440705 (-) 747 WP_000487010.1 DNA repair protein RecO Machinery gene
  CUC43_RS02555 (CUC43_02660) - 440739..440882 (-) 144 WP_000883924.1 YqzL family protein -
  CUC43_RS02560 (CUC43_02665) era 441059..441964 (-) 906 WP_029439945.1 GTPase Era -
  CUC43_RS02565 (CUC43_02670) - 441957..442355 (-) 399 WP_001086294.1 cytidine deaminase -
  CUC43_RS02570 (CUC43_02675) - 442455..442808 (-) 354 WP_029439946.1 diacylglycerol kinase family protein -
  CUC43_RS02575 (CUC43_02680) ybeY 442805..443275 (-) 471 WP_000054692.1 rRNA maturation RNase YbeY -
  CUC43_RS02580 (CUC43_02685) - 443272..445416 (-) 2145 WP_029439947.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 248 a.a.        Molecular weight: 28665.22 Da        Isoelectric Point: 8.0540

>NTDB_id=255344 CUC43_RS02550 WP_000487010.1 439959..440705(-) (recO) [Bacillus thuringiensis LM1212]
MFQKVEGIVIRTTDYGETNKIVTIFSRELGKVSAMARGAKKPKSRLASVSQLMTHGHFLIQMGSGLGTLQQGEIISTMKE
IREDIFLTAYASFIVELTDKATEDKKHNPYLFEMLYQTLHYMCEGVDPEVLSLIYQTKMLPVLGMRPYFDTCAICHQETD
FVAFSVREGGFLCSRHAEQDQYRIPVGEAVHKLLRLFYHFDLHRLGNVSVKDSTKKQMRLVLNTYYDEYCGIYLKSRRFL
EQLDKFQI

Nucleotide


Download         Length: 747 bp        

>NTDB_id=255344 CUC43_RS02550 WP_000487010.1 439959..440705(-) (recO) [Bacillus thuringiensis LM1212]
ATGTTTCAAAAAGTTGAGGGCATCGTTATCCGTACGACAGATTACGGAGAAACGAACAAGATTGTTACAATATTCTCAAG
AGAATTGGGTAAGGTAAGTGCAATGGCAAGAGGAGCGAAAAAACCGAAAAGTCGGTTAGCATCTGTTTCGCAGCTTATGA
CACATGGTCATTTCCTTATTCAAATGGGATCTGGACTTGGAACTTTGCAACAAGGCGAGATTATTTCAACTATGAAAGAA
ATTCGCGAGGATATATTTTTAACTGCTTATGCATCATTTATTGTTGAGTTAACTGATAAAGCAACAGAAGATAAAAAACA
TAATCCATATTTATTTGAAATGTTATATCAAACGTTGCATTATATGTGTGAGGGTGTTGATCCAGAAGTATTATCATTAA
TTTACCAAACAAAAATGCTTCCGGTATTAGGGATGCGCCCGTACTTTGATACATGTGCGATTTGTCATCAAGAAACAGAT
TTTGTCGCCTTCTCTGTTAGGGAGGGCGGTTTTCTGTGCTCACGTCATGCTGAGCAAGATCAGTATCGTATACCAGTGGG
GGAAGCCGTTCATAAATTATTGCGTCTTTTCTACCACTTCGATTTACATAGACTTGGCAATGTATCAGTGAAGGATAGCA
CAAAAAAACAAATGCGTTTAGTATTGAATACATATTATGATGAATATTGCGGGATTTATTTGAAATCAAGACGTTTCTTA
GAACAACTTGATAAGTTTCAAATATAA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-238)

Recombination protein O N terminal (RecO_N)

(1-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q81LT9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

55.466

99.597

0.552

Multiple sequence alignment