Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CUC43_RS01040 Genome accession   NZ_CP024771
Coordinates   179202..179636 (-) Length   144 a.a.
NCBI ID   WP_029438072.1    Uniprot ID   -
Organism   Bacillus thuringiensis LM1212     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 174202..184636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CUC43_RS01025 (CUC43_01075) - 175224..175781 (+) 558 WP_029438069.1 PadR family transcriptional regulator -
  CUC43_RS01030 (CUC43_01080) metE 176450..178738 (+) 2289 WP_029438070.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CUC43_RS01035 (CUC43_01085) comJ 178776..179165 (-) 390 WP_029438071.1 competence protein ComJ -
  CUC43_RS01040 (CUC43_01090) nucA/comI 179202..179636 (-) 435 WP_029438072.1 DNA-entry nuclease Machinery gene
  CUC43_RS01045 (CUC43_01100) - 179873..180622 (+) 750 WP_000388464.1 DUF3967 domain-containing protein -
  CUC43_RS01050 (CUC43_01105) kinB 180676..181950 (-) 1275 WP_029438073.1 sporulation sensor histidine kinase KinB -
  CUC43_RS01055 (CUC43_01110) - 182260..182460 (+) 201 WP_000929285.1 hypothetical protein -
  CUC43_RS01060 (CUC43_01115) - 182584..183741 (+) 1158 WP_000434566.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16024.41 Da        Isoelectric Point: 8.4331

>NTDB_id=255340 CUC43_RS01040 WP_029438072.1 179202..179636(-) (nucA/comI) [Bacillus thuringiensis LM1212]
MKQLKGIIISIIAILSIVVAVYEVLVPEETSIKKTNAYDQILEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFDVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=255340 CUC43_RS01040 WP_029438072.1 179202..179636(-) (nucA/comI) [Bacillus thuringiensis LM1212]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTTTCTATTGTAGTAGCGGTTTATGAAGTACTTGTTCC
AGAGGAAACAAGTATTAAAAAAACAAATGCATATGATCAAATTCTAGAATTTCCGAAAGAGCGATATCCAGAGACAGGGA
AACATATTACGGATGCTATAAAAGAGGGACATTCGGAAGTGTGTACAATCGACCGTGGTGGTGCTGCGGATAGAAGAAAA
TTATCGTTAGCTCCATACCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGTAAAGAAGGCGGAAA
AGGAGCACACATTGAATATATAAGTCCAGCAGATAACCGTGGAGCAGGGTCTTGGGTAGGAAATAAGCTAGATAAATACC
CAGATGGTACGCGTGTGAAATTTGACGTAAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-139)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

71.171

77.083

0.549

Multiple sequence alignment