Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CSW12_RS07710 Genome accession   NZ_CP024655
Coordinates   1445097..1445531 (+) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus cereus strain MLY1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1440097..1450531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CSW12_RS07685 (CSW12_07710) - 1441013..1442176 (-) 1164 WP_023522939.1 aminotransferase A -
  CSW12_RS07690 (CSW12_07715) - 1442297..1442497 (-) 201 WP_000929276.1 hypothetical protein -
  CSW12_RS07695 (CSW12_07720) kinB 1442808..1444082 (+) 1275 WP_000420369.1 sporulation sensor histidine kinase KinB -
  CSW12_RS07700 (CSW12_07725) - 1444121..1444870 (-) 750 WP_000388467.1 DUF3967 domain-containing protein -
  CSW12_RS07710 (CSW12_07735) nucA/comI 1445097..1445531 (+) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  CSW12_RS07715 (CSW12_07740) - 1445568..1445957 (+) 390 WP_000424024.1 competence protein ComJ -
  CSW12_RS07720 (CSW12_07745) metE 1445996..1448284 (-) 2289 WP_089171703.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CSW12_RS07725 (CSW12_07750) - 1448796..1449353 (-) 558 WP_000062063.1 PadR family transcriptional regulator -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=254819 CSW12_RS07710 WP_000811494.1 1445097..1445531(+) (nucA/comI) [Bacillus cereus strain MLY1]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=254819 CSW12_RS07710 WP_000811494.1 1445097..1445531(+) (nucA/comI) [Bacillus cereus strain MLY1]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATTGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCGTATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGGGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATATC
CAGATGGTACGCGTGTGAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment