Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CS557_RS12805 Genome accession   NZ_CP024632
Coordinates   2736244..2737044 (-) Length   266 a.a.
NCBI ID   WP_004908991.1    Uniprot ID   -
Organism   Acinetobacter junii strain lzh-X15     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2731244..2742044
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS557_RS12785 (CS557_12790) - 2732303..2733001 (-) 699 WP_099949198.1 YfgM family protein -
  CS557_RS12790 (CS557_12795) hisS 2733033..2734325 (-) 1293 WP_099949199.1 histidine--tRNA ligase -
  CS557_RS12795 (CS557_12800) ispG 2734322..2735437 (-) 1116 WP_004908985.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CS557_RS12800 (CS557_12805) - 2735462..2736253 (-) 792 WP_004950312.1 helix-turn-helix domain-containing protein -
  CS557_RS12805 (CS557_12810) pilF 2736244..2737044 (-) 801 WP_004908991.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CS557_RS12810 (CS557_12815) rlmN 2737061..2738296 (-) 1236 WP_042891255.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CS557_RS12815 (CS557_12820) ndk 2738418..2738849 (-) 432 WP_003654870.1 nucleoside-diphosphate kinase -
  CS557_RS12820 (CS557_12825) iscX 2738957..2739157 (-) 201 WP_042891252.1 Fe-S cluster assembly protein IscX -
  CS557_RS12825 (CS557_12830) - 2739240..2739791 (-) 552 WP_099949200.1 phosphatase PAP2 family protein -
  CS557_RS12830 (CS557_12835) - 2739819..2741135 (-) 1317 WP_171265915.1 glycosyltransferase family 4 protein -

Sequence

Protein


Download         Length: 266 a.a.        Molecular weight: 30366.44 Da        Isoelectric Point: 9.5600

>NTDB_id=254595 CS557_RS12805 WP_004908991.1 2736244..2737044(-) (pilF) [Acinetobacter junii strain lzh-X15]
MNTTRLKTILATTVVFTVLGLTACQSTTLRKDPEKAVQVRTQLAAEYIRTRDLDSAKRSLDQALKIDPRDATANMMMGVL
LQQEGSKPNMEKAEAYFKRAISSEPDNAQARNNYGTYLYQMQRYNEAIEQFTVAGATLGYEQRYQSLENLGRIYLKLGDV
ANAEKSFKQALQANRNSTISMLELSEIFYLQQNNRDASQLYEQYVRNVGQKNQGARALWIGIRIARANADQLGMQVLVNQ
MRALFPDSPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 801 bp        

>NTDB_id=254595 CS557_RS12805 WP_004908991.1 2736244..2737044(-) (pilF) [Acinetobacter junii strain lzh-X15]
TTGAATACAACTCGACTAAAAACGATTTTAGCTACTACTGTTGTGTTTACTGTATTAGGTTTGACTGCCTGCCAATCAAC
AACGTTAAGAAAAGATCCAGAAAAGGCAGTACAAGTCCGAACTCAACTCGCTGCTGAATATATTCGAACACGTGATTTGG
ATTCAGCGAAACGCTCTCTTGATCAAGCTTTAAAAATTGATCCACGTGATGCTACTGCCAATATGATGATGGGTGTTTTG
TTGCAACAAGAAGGCAGCAAACCAAACATGGAGAAAGCAGAAGCTTATTTTAAGCGAGCAATTTCTTCAGAACCCGATAA
TGCTCAAGCTCGTAATAATTACGGTACATATTTGTATCAAATGCAACGTTATAATGAAGCGATTGAGCAATTTACCGTTG
CAGGTGCGACGTTAGGTTACGAACAGCGTTACCAATCGTTGGAAAACTTGGGTCGAATCTATTTAAAACTGGGTGATGTT
GCGAATGCTGAAAAATCATTCAAGCAAGCCTTACAAGCAAATCGAAATTCGACAATTTCAATGTTAGAGTTGTCCGAAAT
TTTCTATTTGCAACAAAATAATAGAGATGCATCGCAACTTTATGAACAATACGTTCGTAATGTCGGGCAGAAAAATCAAG
GTGCTCGTGCACTTTGGATCGGTATACGCATTGCTCGAGCGAATGCGGATCAATTAGGAATGCAAGTATTAGTGAATCAA
ATGCGTGCTTTATTTCCTGATAGTCCAGAATATCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAATA
A

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(39-69)

Tetratricopeptide repeat (TPR_8)

(145-176)

Tetratricopeptide repeat (TPR_16)

(90-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

79.026

100

0.793
  pilF Acinetobacter baylyi ADP1

68.045

100

0.68

Multiple sequence alignment