Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   CTZ23_RS12550 Genome accession   NZ_CP024620
Coordinates   2586699..2587496 (-) Length   265 a.a.
NCBI ID   WP_104989096.1    Uniprot ID   -
Organism   Acinetobacter indicus strain SGAir0564     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2581699..2592496
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CTZ23_RS12530 (CTZ23_12590) - 2582742..2583446 (-) 705 WP_005182401.1 YfgM family protein -
  CTZ23_RS12535 (CTZ23_12595) hisS 2583477..2584772 (-) 1296 WP_045795989.1 histidine--tRNA ligase -
  CTZ23_RS12540 (CTZ23_12600) ispG 2584776..2585897 (-) 1122 WP_005182396.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  CTZ23_RS12545 (CTZ23_12605) - 2585920..2586708 (-) 789 WP_104989095.1 helix-turn-helix domain-containing protein -
  CTZ23_RS12550 (CTZ23_12610) pilF 2586699..2587496 (-) 798 WP_104989096.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  CTZ23_RS12555 (CTZ23_12615) rlmN 2587532..2588767 (-) 1236 WP_104989097.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  CTZ23_RS12560 (CTZ23_12620) ndk 2588890..2589321 (-) 432 WP_005182388.1 nucleoside-diphosphate kinase -
  CTZ23_RS12565 (CTZ23_12625) iscX 2589430..2589630 (-) 201 WP_005182385.1 Fe-S cluster assembly protein IscX -
  CTZ23_RS12570 (CTZ23_12630) - 2589725..2590276 (-) 552 WP_104989098.1 phosphatase PAP2 family protein -
  CTZ23_RS12575 (CTZ23_12635) - 2590309..2591592 (-) 1284 WP_104989099.1 glycosyltransferase family 4 protein -
  CTZ23_RS12580 (CTZ23_12640) - 2591760..2592179 (-) 420 WP_005182377.1 hypothetical protein -

Sequence

Protein


Download         Length: 265 a.a.        Molecular weight: 29914.89 Da        Isoelectric Point: 8.7723

>NTDB_id=254443 CTZ23_RS12550 WP_104989096.1 2586699..2587496(-) (pilF) [Acinetobacter indicus strain SGAir0564]
MYKFTTISTLCVAAWMSTGCQTTTPTLSPKDPEKAIQVRTQLAAEYLKSGDLDSAKRALDQALETDSRDSMANMMMGVLL
QQEGSKLNLEKADTYFKRAISADPNNAQARNNYGTYLYQVERYNDAIEQLSIAGATLGYDQRFRALENVGRIYLKLGDMA
NAEKLFKQALQANRGSYVSMLELAEIFYFNQQTPAASRMYEQFVRAVGQKNQGARALWIGIRVARANGDQLGQQVLVNQL
RALFPESQEYQRYLQLQHSTEAVWK

Nucleotide


Download         Length: 798 bp        

>NTDB_id=254443 CTZ23_RS12550 WP_104989096.1 2586699..2587496(-) (pilF) [Acinetobacter indicus strain SGAir0564]
ATGTATAAATTCACAACCATCAGCACACTGTGTGTAGCTGCATGGATGAGCACCGGCTGTCAAACCACAACTCCTACTTT
AAGTCCGAAAGATCCGGAAAAGGCAATTCAGGTGCGTACCCAGCTGGCCGCCGAGTATTTAAAATCGGGTGATTTAGACT
CGGCCAAACGTGCCCTGGATCAGGCGCTGGAAACAGATTCGCGTGATTCCATGGCCAATATGATGATGGGCGTGCTGCTA
CAGCAAGAAGGCAGCAAGCTGAATCTGGAGAAGGCCGATACGTATTTTAAGCGTGCCATCTCTGCCGATCCGAACAATGC
GCAGGCGCGTAACAACTATGGTACGTATTTGTATCAGGTTGAACGCTATAATGATGCCATTGAGCAGCTGAGTATTGCCG
GGGCAACACTCGGTTATGATCAACGTTTTCGTGCATTGGAAAACGTGGGGCGGATTTACCTGAAACTGGGCGATATGGCA
AATGCTGAAAAATTATTCAAGCAGGCCCTGCAGGCAAACCGTGGCTCCTATGTTTCAATGTTAGAGTTGGCGGAAATTTT
TTATTTCAACCAGCAGACTCCTGCCGCATCCAGAATGTATGAACAGTTTGTGCGTGCTGTAGGACAAAAAAATCAAGGTG
CGCGTGCCTTATGGATTGGGATCCGTGTTGCACGTGCAAATGGCGATCAGTTAGGACAGCAGGTGCTGGTCAATCAGCTT
CGGGCATTGTTCCCTGAAAGCCAGGAATATCAACGTTATTTGCAATTACAGCACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(144-174)

Tetratricopeptide repeat (TPR_17)

(96-128)

Tetratricopeptide repeat (TPR_16)

(42-76)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

77.606

97.736

0.758
  pilF Acinetobacter baylyi ADP1

71.747

100

0.728

Multiple sequence alignment