Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   SAUSA300_RS08215 Genome accession   NC_007793
Coordinates   1656113..1657087 (-) Length   324 a.a.
NCBI ID   WP_000697228.1    Uniprot ID   A0A0D3Q868
Organism   Staphylococcus aureus subsp. aureus USA300_FPR3757     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1630942..1657087 1656113..1657087 within 0


Gene organization within MGE regions


Location: 1630942..1657087
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAUSA300_RS08060 - 1630942..1631478 (-) 537 WP_077442703.1 IS30 family transposase -
  SAUSA300_RS08065 - 1631459..1631962 (-) 504 WP_000746375.1 transposase -
  SAUSA300_RS08070 (SAUSA300_1478) - 1632071..1632427 (-) 357 WP_001255379.1 cystatin-like fold lipoprotein -
  SAUSA300_RS08075 (SAUSA300_1479) - 1632483..1633073 (-) 591 WP_000810443.1 hypothetical protein -
  SAUSA300_RS08080 (SAUSA300_1480) - 1633080..1634126 (-) 1047 WP_000247465.1 CHAP domain-containing protein -
  SAUSA300_RS08085 (SAUSA300_1481) - 1634116..1635963 (-) 1848 WP_000681154.1 CD3337/EF1877 family mobilome membrane protein -
  SAUSA300_RS08090 (SAUSA300_1482) - 1635968..1637326 (-) 1359 WP_001251211.1 FtsK/SpoIIIE domain-containing protein -
  SAUSA300_RS08095 (SAUSA300_1483) - 1637380..1639875 (-) 2496 WP_001049264.1 ATP-binding protein -
  SAUSA300_RS08100 (SAUSA300_1484) - 1639910..1640293 (-) 384 WP_000358144.1 TcpE family conjugal transfer membrane protein -
  SAUSA300_RS08105 (SAUSA300_1485) - 1640305..1640565 (-) 261 WP_000015639.1 TcpD family membrane protein -
  SAUSA300_RS08110 (SAUSA300_1486) - 1640570..1641625 (-) 1056 WP_000692003.1 conjugal transfer protein -
  SAUSA300_RS08115 (SAUSA300_1487) - 1641686..1642777 (-) 1092 WP_000172939.1 replication initiation factor domain-containing protein -
  SAUSA300_RS08120 (SAUSA300_1488) - 1642952..1643254 (-) 303 WP_000386891.1 hypothetical protein -
  SAUSA300_RS08125 (SAUSA300_1489) - 1643268..1643588 (-) 321 WP_000805733.1 DUF961 family protein -
  SAUSA300_RS08130 - 1643739..1644023 (-) 285 WP_000134545.1 hypothetical protein -
  SAUSA300_RS08135 (SAUSA300_1490) efp 1644418..1644975 (-) 558 WP_000626504.1 elongation factor P -
  SAUSA300_RS08140 (SAUSA300_1491) - 1645001..1646062 (-) 1062 WP_000087107.1 M24 family metallopeptidase -
  SAUSA300_RS08145 (SAUSA300_1492) - 1646167..1646748 (+) 582 WP_000737686.1 hypothetical protein -
  SAUSA300_RS08150 (SAUSA300_1493) - 1646762..1646980 (+) 219 WP_001244298.1 SA1362 family protein -
  SAUSA300_RS08155 (SAUSA300_1494) - 1647044..1647874 (-) 831 WP_000141108.1 lipoate--protein ligase family protein -
  SAUSA300_RS08160 (SAUSA300_1495) - 1648032..1648418 (+) 387 WP_001276571.1 rhodanese-like domain-containing protein -
  SAUSA300_RS08165 (SAUSA300_1496) gcvPB 1648783..1650255 (-) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  SAUSA300_RS08170 (SAUSA300_1497) gcvPA 1650248..1651594 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  SAUSA300_RS08175 (SAUSA300_1498) gcvT 1651614..1652705 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  SAUSA300_RS08180 (SAUSA300_1499) - 1652864..1653388 (-) 525 WP_001015117.1 shikimate kinase -
  SAUSA300_RS08185 - 1653378..1653524 (-) 147 WP_001789879.1 hypothetical protein -
  SAUSA300_RS08190 (SAUSA300_1500) comGF 1653621..1654118 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  SAUSA300_RS08195 comGE 1654036..1654335 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  SAUSA300_RS08200 (SAUSA300_1501) comGD 1654322..1654768 (-) 447 WP_001791635.1 competence type IV pilus minor pilin ComGD Machinery gene
  SAUSA300_RS08205 (SAUSA300_1502) comGC 1654746..1655057 (-) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  SAUSA300_RS08210 (SAUSA300_1503) comGB 1655071..1656141 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAUSA300_RS08215 (SAUSA300_1504) comGA 1656113..1657087 (-) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36865.01 Da        Isoelectric Point: 9.0399

>NTDB_id=25399 SAUSA300_RS08215 WP_000697228.1 1656113..1657087(-) (comGA) [Staphylococcus aureus subsp. aureus USA300_FPR3757]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRLLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=25399 SAUSA300_RS08215 WP_000697228.1 1656113..1657087(-) (comGA) [Staphylococcus aureus subsp. aureus USA300_FPR3757]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCTATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D3Q868

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

99.074

100

0.991
  comGA Staphylococcus aureus N315

99.074

100

0.991

Multiple sequence alignment