Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   CS376_RS13135 Genome accession   NZ_CP024203
Coordinates   2656416..2657183 (-) Length   255 a.a.
NCBI ID   WP_012118014.1    Uniprot ID   A7Z6U2
Organism   Bacillus velezensis strain NKG-1     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2651416..2662183
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS376_RS13115 - 2651618..2652430 (-) 813 WP_003152991.1 pyruvate, water dikinase regulatory protein -
  CS376_RS13120 ccpN 2652456..2653094 (-) 639 WP_003152989.1 transcriptional regulator CcpN -
  CS376_RS13125 glyS 2653195..2655234 (-) 2040 WP_012118013.1 glycine--tRNA ligase subunit beta -
  CS376_RS13130 glyQ 2655227..2656114 (-) 888 WP_003152984.1 glycine--tRNA ligase subunit alpha -
  CS376_RS13135 recO 2656416..2657183 (-) 768 WP_012118014.1 DNA repair protein RecO Machinery gene
  CS376_RS13140 - 2657220..2657363 (-) 144 WP_007408286.1 YqzL family protein -
  CS376_RS13145 era 2657519..2658424 (-) 906 WP_007408285.1 GTPase Era -
  CS376_RS13150 - 2658405..2658815 (-) 411 WP_003152976.1 cytidine deaminase -
  CS376_RS13155 - 2658939..2659313 (-) 375 WP_043021628.1 diacylglycerol kinase family protein -
  CS376_RS13160 ybeY 2659294..2659767 (-) 474 WP_012118016.1 rRNA maturation RNase YbeY -
  CS376_RS13165 - 2659768..2661900 (-) 2133 WP_007408284.1 HD family phosphohydrolase -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29310.95 Da        Isoelectric Point: 8.5625

>NTDB_id=252914 CS376_RS13135 WP_012118014.1 2656416..2657183(-) (recO) [Bacillus velezensis strain NKG-1]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGARKSASRLSAVSQPFLYGSFLMQRTSGLGTLQQGEMILSMRT
IREDLFLTAYAAFIAELTDKGTEEKKPNPYLFELILESFKRLNDGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSGEG
TFHFSIRDNGFICHRCFEKDPYRVPMSPQTARLLRLFYYFDIGRLGSVSLKQETKNEIKRVIDLYYEEYSGLYLKSKRFL
DQMESMKNLLDENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=252914 CS376_RS13135 WP_012118014.1 2656416..2657183(-) (recO) [Bacillus velezensis strain NKG-1]
ATGCTGACTAAATGTGAAGGAATCGTGCTCCGTACGAATGACTACGGGGAGACGAATAAAATCGTGACATTGCTGACGAG
GGAACACGGCAAAATCGGCGTCATGGCAAGGGGCGCCAGGAAGTCAGCCAGCCGGCTGTCAGCAGTCAGCCAGCCGTTTT
TGTACGGGTCATTTTTAATGCAAAGAACATCCGGACTCGGCACGCTCCAGCAGGGTGAAATGATTCTCAGCATGAGAACG
ATCAGAGAAGATTTATTTTTGACGGCGTATGCCGCTTTTATCGCTGAACTCACTGACAAAGGAACGGAAGAGAAGAAACC
GAACCCCTATTTATTCGAATTGATTCTCGAATCCTTTAAGAGGCTGAATGACGGGACTGACCCGGACGTGATCACCTTCA
TCGTGCAGATGAAAATGCTCGGCGTCATGGGGCTGTATCCGGAGCTGAATCATTGTGTCCACTGCAAAAGCGGGGAAGGG
ACGTTTCATTTTTCGATAAGAGATAACGGGTTTATCTGCCACCGCTGCTTTGAAAAGGATCCGTACAGAGTTCCGATGTC
GCCGCAAACGGCAAGGCTGCTAAGGCTTTTTTATTACTTTGATATCGGAAGGCTCGGCAGCGTCTCGTTAAAACAGGAAA
CCAAAAACGAAATTAAACGGGTCATTGATCTGTATTACGAGGAATATTCAGGACTTTATTTAAAGTCAAAACGGTTTTTA
GATCAGATGGAAAGCATGAAGAACCTTTTGGATGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O C terminal (RecO_C)

(82-237)

Recombination protein O N terminal (RecO_N)

(1-75)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7Z6U2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

88.627

100

0.886

Multiple sequence alignment