Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   CS376_RS00020 Genome accession   NZ_CP024203
Coordinates   3028..4140 (+) Length   370 a.a.
NCBI ID   WP_099566347.1    Uniprot ID   -
Organism   Bacillus velezensis strain NKG-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1..9140
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CS376_RS00005 dnaA 1..1341 (+) 1341 WP_099566346.1 chromosomal replication initiator protein DnaA -
  CS376_RS00010 dnaN 1526..2662 (+) 1137 WP_007409909.1 DNA polymerase III subunit beta -
  CS376_RS00015 rlbA 2797..3012 (+) 216 WP_004392910.1 ribosome maturation protein RlbA -
  CS376_RS00020 recF 3028..4140 (+) 1113 WP_099566347.1 DNA replication/repair protein RecF Machinery gene
  CS376_RS00025 remB 4158..4403 (+) 246 WP_004392908.1 extracellular matrix regulator RemB -
  CS376_RS00030 gyrB 4460..6376 (+) 1917 WP_011996153.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  CS376_RS00035 gyrA 6592..9051 (+) 2460 WP_015239009.1 DNA gyrase subunit A -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42460.39 Da        Isoelectric Point: 7.0882

>NTDB_id=252870 CS376_RS00020 WP_099566347.1 3028..4140(+) (recF) [Bacillus velezensis strain NKG-1]
MYIQNLELTSYRNYERTELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDEDYAKIEGRVMKRNGD
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSAVYLYDLSLYQKILSQR
NHFLKQLQSRKQTDRTMLDVLTDQLIEAAAKVVAKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALDVSDPKDLSKI
GNSYQESFSKLKEKEIERGVTLFGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHDTLHQAGMFRVENGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=252870 CS376_RS00020 WP_099566347.1 3028..4140(+) (recF) [Bacillus velezensis strain NKG-1]
TTGTATATCCAAAATTTAGAATTAACATCCTACCGTAATTATGAGCGCACTGAGCTTCAATTCGAAAACAAGGTGAATGT
GATCATCGGGGAAAATGCCCAAGGCAAAACCAATCTGATGGAAGCCATCTATGTCCTTTCGATGGCAAAATCACACCGCA
CCTCAAATGACAAAGAACTTATACGGTGGGACGAAGACTATGCTAAAATAGAAGGAAGAGTGATGAAGCGAAACGGGGAT
ATCCCGATGCAGCTTGTCATCTCCAAAAAAGGTAAAAAGGGCAAGGTCAATCATATAGAGCAGCAAAAACTCAGTCAGTA
TGTCGGGGCGCTGAACACCATTATGTTTGCCCCTGAGGATTTAAATCTCGTCAAAGGAAGCCCCCAGGTGAGGAGGAGAT
TTCTCGATATGGAGATCGGGCAGGTTTCTGCCGTTTATCTGTATGATTTATCTCTTTATCAAAAAATCCTTTCACAGCGG
AATCATTTTCTGAAACAGCTGCAAAGCCGGAAACAGACTGACCGGACCATGCTTGACGTTCTCACTGATCAGCTGATTGA
AGCAGCCGCAAAAGTTGTCGCCAAACGTCTGCAGTTTACGGCGCAGCTTGAAAAATGGGCGCAGCCGATCCATTCCGGCA
TTTCCCGGGGACTTGAAGAACTGACGCTTAAGTATCACACGGCGCTTGATGTATCAGATCCCAAAGATTTGTCGAAAATA
GGTAACAGCTACCAGGAGTCTTTTTCGAAATTAAAAGAAAAAGAAATAGAACGAGGCGTCACATTATTCGGACCGCATCG
GGATGATGTCCTTTTTTATGTGAACGGACGCGATGTGCAGACGTACGGTTCACAGGGACAGCAGCGTACGACGGCTTTGT
CTTTGAAGCTGGCCGAAATAGATTTGATTCATGAAGAAATCGGAGAATACCCCATTTTACTTTTGGATGATGTACTAAGC
GAGCTGGATGATTACCGACAGTCCCATTTGCTTCATACGATCCAAGGCCGTGTGCAGACATTTGTCACCACGACAAGCGT
GGATGGCATTGACCACGATACCTTACATCAAGCAGGAATGTTCCGTGTGGAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

94.054

100

0.941

Multiple sequence alignment