Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CG474_RS14850 Genome accession   NZ_CP024120
Coordinates   2848234..2848668 (-) Length   144 a.a.
NCBI ID   WP_012095165.1    Uniprot ID   A7GS32
Organism   Bacillus cytotoxicus strain CH_1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2843234..2853668
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG474_RS14835 (CG474_014765) - 2843331..2844866 (+) 1536 WP_048721822.1 MDR family MFS transporter -
  CG474_RS14840 (CG474_014770) metE 2845456..2847744 (+) 2289 WP_048721820.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CG474_RS14845 (CG474_014775) comJ 2847823..2848212 (-) 390 WP_012095164.1 competence protein ComJ -
  CG474_RS14850 (CG474_014780) nucA/comI 2848234..2848668 (-) 435 WP_012095165.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CG474_RS14855 (CG474_014785) - 2848909..2849655 (+) 747 WP_012095166.1 DUF3967 domain-containing protein -
  CG474_RS14860 (CG474_014790) - 2849729..2851003 (-) 1275 WP_012095167.1 sensor histidine kinase -
  CG474_RS14865 (CG474_014795) - 2851312..2851512 (+) 201 WP_048721818.1 hypothetical protein -
  CG474_RS14870 (CG474_014800) - 2851611..2852774 (+) 1164 WP_048721816.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16090.47 Da        Isoelectric Point: 9.4939

>NTDB_id=252258 CG474_RS14850 WP_012095165.1 2848234..2848668(-) (nucA/comI) [Bacillus cytotoxicus strain CH_1]
MKQLKGIIISIIAVLSILVAVYEALIPTEQAKQKDGTYDQVLEFPKDRYPETGKHIENAIKEGHSKICTIDRNGAGDRRK
QSLAPYPPKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYQDGTRVKFVVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=252258 CG474_RS14850 WP_012095165.1 2848234..2848668(-) (nucA/comI) [Bacillus cytotoxicus strain CH_1]
ATGAAACAATTGAAAGGGATTATTATTTCAATCATTGCGGTTCTTTCTATTTTAGTGGCTGTTTATGAAGCGCTTATACC
GACAGAACAAGCAAAACAAAAGGACGGTACATATGATCAAGTTCTAGAATTTCCGAAAGATCGTTATCCAGAAACCGGAA
AGCATATAGAAAATGCAATAAAGGAAGGACATTCTAAAATCTGTACGATTGATAGAAATGGAGCTGGTGACAGACGAAAG
CAATCTTTAGCTCCATATCCACCAAAAAAAGGATACGATCGTGATGAATGGCCGATGGCAATGTGTAAGGAAGGAGGCAA
GGGGGCTCATATTGAATATATTAGTCCGGCTGATAATAGAGGAGCTGGCTCATGGGTCGGTAATAAATTGGATAAATACC
AAGATGGTACACGTGTGAAATTTGTAGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GS32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.333

83.333

0.528

Multiple sequence alignment