Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CG479_RS14445 Genome accession   NZ_CP024109
Coordinates   2784330..2784764 (-) Length   144 a.a.
NCBI ID   WP_012095165.1    Uniprot ID   A7GS32
Organism   Bacillus cytotoxicus strain CH_13     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2779330..2789764
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG479_RS14430 (CG479_014410) - 2779427..2780962 (+) 1536 WP_094396540.1 MDR family MFS transporter -
  CG479_RS14435 (CG479_014415) metE 2781552..2783840 (+) 2289 WP_094396541.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  CG479_RS14440 (CG479_014420) comJ 2783919..2784308 (-) 390 WP_012095164.1 competence protein ComJ -
  CG479_RS14445 (CG479_014425) nucA/comI 2784330..2784764 (-) 435 WP_012095165.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CG479_RS14450 (CG479_014430) - 2785005..2785751 (+) 747 WP_012095166.1 DUF3967 domain-containing protein -
  CG479_RS14455 (CG479_014435) - 2785825..2787099 (-) 1275 WP_012095167.1 sensor histidine kinase -
  CG479_RS14460 (CG479_014440) - 2787408..2787608 (+) 201 WP_048721818.1 hypothetical protein -
  CG479_RS14465 (CG479_014445) - 2787707..2788870 (+) 1164 WP_048721816.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16090.47 Da        Isoelectric Point: 9.4939

>NTDB_id=252068 CG479_RS14445 WP_012095165.1 2784330..2784764(-) (nucA/comI) [Bacillus cytotoxicus strain CH_13]
MKQLKGIIISIIAVLSILVAVYEALIPTEQAKQKDGTYDQVLEFPKDRYPETGKHIENAIKEGHSKICTIDRNGAGDRRK
QSLAPYPPKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYQDGTRVKFVVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=252068 CG479_RS14445 WP_012095165.1 2784330..2784764(-) (nucA/comI) [Bacillus cytotoxicus strain CH_13]
ATGAAACAATTGAAAGGGATTATTATTTCAATCATTGCGGTTCTTTCTATTTTAGTGGCTGTTTATGAAGCGCTTATACC
GACAGAACAAGCAAAACAAAAGGACGGTACATATGATCAAGTTCTAGAATTTCCGAAAGATCGTTATCCAGAAACCGGAA
AGCATATAGAAAATGCAATAAAGGAAGGACATTCTAAAATCTGTACGATTGATAGAAATGGAGCTGGTGACAGACGAAAG
CAATCTTTAGCTCCATATCCACCAAAAAAAGGATACGATCGTGATGAATGGCCGATGGCAATGTGTAAGGAAGGAGGCAA
GGGGGCTCATATTGAATATATTAGTCCGGCTGATAATAGAGGAGCTGGCTCATGGGTCGGTAATAAATTGGATAAATACC
AAGATGGTACACGTGTGAAATTTGTAGTGAAATAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7GS32

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

63.333

83.333

0.528

Multiple sequence alignment