Detailed information NTG0252009

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: DEF50_RS10490
• Genome accession: NZ_CP029180
• Coordinates: 1949525..1950517 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain H9Ecoli
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 1944525..1955517

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DEF50_RS10470 (DEF50_10470)	otnK	1945299..1946561 (+)	1263	WP_000590403.1	3-oxo-tetronate kinase	-
DEF50_RS10475 (DEF50_10475)	ygbL	1946558..1947196 (+)	639	WP_001278994.1	aldolase	-
DEF50_RS10480 (DEF50_10480)	ygbM	1947201..1947977 (+)	777	WP_001136934.1	HPr family phosphocarrier protein	-
DEF50_RS10485 (DEF50_10485)	ygbN	1948067..1949431 (+)	1365	WP_000104441.1	GntP family transporter	-
DEF50_RS10490 (DEF50_10490)	rpoS	1949525..1950517 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
DEF50_RS10495 (DEF50_10495)	nlpD	1950580..1951674 (-)	1095	WP_224692580.1	murein hydrolase activator NlpD	-
DEF50_RS10500 (DEF50_10500)	pcm	1951859..1952485 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
DEF50_RS10505 (DEF50_10505)	surE	1952479..1953240 (-)	762	WP_001295182.1	5'/3'-nucleotidase SurE	-
DEF50_RS10510 (DEF50_10510)	truD	1953221..1954270 (-)	1050	WP_000568946.1	tRNA pseudouridine(13) synthase TruD	-
DEF50_RS10515 (DEF50_10515)	ispF	1954267..1954746 (-)	480	WP_072645516.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
DEF50_RS10520 (DEF50_10520)	ispD	1954746..1955456 (-)	711	WP_000246135.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=252009 DEF50_RS10490 WP_000081550.1 1949525..1950517(-) (rpoS) [Escherichia coli strain H9Ecoli]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=252009 DEF50_RS10490 WP_000081550.1 1949525..1950517(-) (rpoS) [Escherichia coli strain H9Ecoli]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGTTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTTATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATTGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCATGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAACGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGTGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715