Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   CG481_RS20575 Genome accession   NZ_CP024104
Coordinates   3896816..3898165 (-) Length   449 a.a.
NCBI ID   WP_048723495.1    Uniprot ID   -
Organism   Bacillus cytotoxicus strain CH_23     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3891816..3903165
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CG481_RS20555 (CG481_020465) secA 3892026..3894536 (-) 2511 WP_048723492.1 preprotein translocase subunit SecA -
  CG481_RS20560 (CG481_020470) hpf 3894899..3895438 (-) 540 WP_012096178.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  CG481_RS20565 (CG481_020480) - 3895792..3895989 (-) 198 WP_012096179.1 cold shock domain-containing protein -
  CG481_RS20570 (CG481_020485) - 3896112..3896816 (-) 705 WP_048723493.1 ComF family protein -
  CG481_RS20575 (CG481_020490) comFA 3896816..3898165 (-) 1350 WP_048723495.1 DEAD/DEAH box helicase Machinery gene
  CG481_RS20580 (CG481_020495) - 3898290..3899519 (-) 1230 WP_048723497.1 C40 family peptidase -
  CG481_RS20585 (CG481_020500) - 3899790..3900104 (-) 315 WP_012096183.1 winged helix-turn-helix transcriptional regulator -
  CG481_RS20590 (CG481_020505) - 3900389..3901231 (-) 843 WP_094398369.1 DegV family protein -
  CG481_RS20595 (CG481_020510) - 3901622..3902260 (+) 639 WP_012096185.1 YigZ family protein -

Sequence

Protein


Download         Length: 449 a.a.        Molecular weight: 51530.44 Da        Isoelectric Point: 9.7376

>NTDB_id=251987 CG481_RS20575 WP_048723495.1 3896816..3898165(-) (comFA) [Bacillus cytotoxicus strain CH_23]
MIQKGRQMLLHELSWSKEQLHDFISRGEIVLEKGIVRKNSNYICQRCGNQEKRLFASFLCKRCNQVCAYCRKCIMMGRVS
ECTMLVRSVCEGKQECFPHALQWKGVLSPGQNRAAKGVVEAIKQKESFFIWAVCGAGKTEMLFSGIDEALKNGKRVCIAT
PRKDVVLELAPRLQEVFPDIPFATLYGGSKEYEKEAMLIVTTTHQLLRYYRAFDVMIIDEIDAFPYVMDHMLHEAVRKAA
KEDASYIYLTATPEEKWKQSVQNGKQKGVIISGRYHRHPLPVPKLKWCGNWRKLLRRKQIPQVLLDWLLIYLQKHYPIFL
FVPHICYVEEVTAVLQRLHHKITGVHAEDPMRKEKVEAFRKGDIPLLITTTILERGVTVANLQVAVLGAEEDIFSESALV
QIAGRVGRSSHNPKGEVIYFHYGKTKAMTAAKKHIQFMNKRAKREGLID

Nucleotide


Download         Length: 1350 bp        

>NTDB_id=251987 CG481_RS20575 WP_048723495.1 3896816..3898165(-) (comFA) [Bacillus cytotoxicus strain CH_23]
ATGATACAAAAAGGAAGACAAATGTTATTGCATGAACTTTCATGGTCAAAAGAACAGTTGCACGATTTCATAAGTAGAGG
GGAGATTGTATTAGAAAAAGGGATTGTTCGAAAAAATTCCAATTATATATGCCAACGTTGCGGGAATCAAGAGAAACGTC
TATTTGCATCATTTTTATGCAAAAGGTGTAATCAAGTATGTGCGTATTGCCGGAAGTGTATTATGATGGGACGAGTAAGC
GAATGTACGATGCTTGTTCGAAGTGTTTGCGAAGGGAAACAAGAATGTTTTCCTCATGCCTTGCAGTGGAAAGGAGTATT
GTCACCGGGGCAAAATAGAGCTGCAAAGGGGGTAGTAGAAGCGATTAAACAGAAAGAGTCCTTTTTTATTTGGGCGGTAT
GTGGTGCTGGAAAAACGGAGATGTTGTTTTCTGGCATAGATGAAGCGCTTAAAAACGGTAAGCGAGTTTGTATAGCGACG
CCTCGAAAAGATGTTGTATTAGAATTAGCTCCACGACTACAAGAAGTGTTTCCAGATATACCATTTGCGACATTATACGG
AGGCAGTAAAGAATATGAAAAAGAAGCGATGCTTATCGTAACTACAACTCATCAGTTGCTTCGCTATTATAGGGCGTTTG
ATGTCATGATTATAGATGAAATTGATGCTTTTCCGTATGTGATGGATCATATGTTACATGAGGCCGTAAGAAAAGCGGCG
AAGGAGGATGCTTCCTATATTTATTTAACAGCAACTCCAGAAGAGAAATGGAAACAGAGTGTGCAAAATGGTAAACAAAA
AGGTGTTATTATATCAGGACGTTATCATCGCCATCCATTACCGGTTCCTAAATTAAAATGGTGTGGAAATTGGAGAAAAC
TACTTCGACGTAAACAAATTCCTCAAGTTCTATTGGACTGGCTTCTTATATATCTACAAAAACATTATCCTATCTTTTTA
TTTGTTCCTCATATTTGTTATGTAGAGGAAGTAACGGCTGTGTTACAAAGATTACATCATAAAATTACTGGTGTTCATGC
AGAAGATCCAATGAGAAAAGAGAAAGTAGAGGCATTTCGTAAAGGGGATATACCATTACTCATAACTACAACTATATTGG
AGAGAGGGGTAACGGTCGCGAATTTACAAGTAGCTGTGCTAGGAGCAGAAGAGGATATTTTTTCTGAAAGTGCACTGGTT
CAGATAGCGGGCCGCGTTGGTAGAAGTTCTCACAATCCAAAGGGGGAAGTCATTTATTTTCATTATGGAAAAACGAAAGC
AATGACTGCTGCGAAAAAGCATATTCAATTTATGAATAAAAGGGCTAAACGAGAAGGGTTGATAGATTGA

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(312-409)

Type III restriction enzyme, res subunit (ResIII)

(107-254)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

50.113

98.218

0.492
  comFA Latilactobacillus sakei subsp. sakei 23K

38.626

93.987

0.363

Multiple sequence alignment