Detailed information    

insolico Bioinformatically predicted

Overview

Name   sbcB   Type   Machinery gene
Locus tag   DEF50_RS00565 Genome accession   NZ_CP029180
Coordinates   115045..116469 (-) Length   474 a.a.
NCBI ID   WP_089537653.1    Uniprot ID   -
Organism   Escherichia coli strain H9Ecoli     
Function   promote homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 110045..121469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DEF50_RS00535 (DEF50_00535) yeeZ 110100..110924 (+) 825 WP_001567684.1 SDR family oxidoreductase -
  DEF50_RS00540 (DEF50_00540) yeeY 110970..111899 (+) 930 WP_000803360.1 LysR substrate-binding domain-containing protein -
  DEF50_RS00545 (DEF50_00545) yoeI 112114..112176 (+) 63 WP_010723108.1 membrane protein YoeI -
  DEF50_RS00550 (DEF50_00550) plaP 112166..113524 (+) 1359 WP_000019197.1 putrescine/proton symporter PlaP -
  DEF50_RS00555 (DEF50_00555) tsuA 113703..114761 (+) 1059 WP_072645364.1 thiosulfate utilization transporter TsuA/YeeE -
  DEF50_RS00560 (DEF50_00560) tsuB 114775..115002 (+) 228 WP_000985273.1 thiosulfate utilization sulfurtransferase TsuB/YeeD -
  DEF50_RS00565 (DEF50_00565) sbcB 115045..116469 (-) 1425 WP_089537653.1 exodeoxyribonuclease I Machinery gene
  DEF50_RS00570 (DEF50_00570) - 116651..117829 (+) 1179 WP_001007954.1 site-specific integrase -
  DEF50_RS00575 (DEF50_00575) - 117810..118001 (-) 192 WP_000132739.1 AlpA family phage regulatory protein -
  DEF50_RS00580 (DEF50_00580) - 118083..118382 (-) 300 WP_157913690.1 hypothetical protein -
  DEF50_RS00585 (DEF50_00585) - 118630..119262 (-) 633 WP_157777018.1 NYN domain-containing protein -
  DEF50_RS00590 (DEF50_00590) - 119542..119826 (-) 285 Protein_115 ead/Ea22-like family protein -
  DEF50_RS00595 (DEF50_00595) - 119823..119990 (-) 168 WP_108990981.1 DUF2737 family protein -
  DEF50_RS00600 (DEF50_00600) - 120001..120297 (-) 297 WP_108990871.1 phage anti-RecBCD protein -
  DEF50_RS00605 (DEF50_00605) - 120321..120908 (-) 588 WP_103744190.1 DUF669 domain-containing protein -

Sequence

Protein


Download         Length: 474 a.a.        Molecular weight: 54325.57 Da        Isoelectric Point: 5.4700

>NTDB_id=251982 DEF50_RS00565 WP_089537653.1 115045..116469(-) (sbcB) [Escherichia coli strain H9Ecoli]
MTGTDKQPTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIGEPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENE
AAFAARIHSLFTVPKTCILGYNNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGL
PSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKPLVHVSGMFGAWRG
NTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDSDTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDAD
RLGINRQHCLDNLRILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDK
RIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQMLAQQYADDKEKVALLKALWQYAEEIV

Nucleotide


Download         Length: 1425 bp        

>NTDB_id=251982 DEF50_RS00565 WP_089537653.1 115045..116469(-) (sbcB) [Escherichia coli strain H9Ecoli]
ATGACAGGCACAGATAAACAACCTACCTTCCTCTTTCACGATTACGAAACCTTTGGCACGCACCCCGCGTTAGATCGCCC
TGCACAGTTCGCAGCCATTCGCACCGATAGCGAATTCAATGTCATCGGCGAACCCGAAGTCTTTTACTGCAAGCCCGCGG
ATGACTATTTGCCCCAGCCTGGAGCAGTATTAATTACCGGTATTACCCCGCAGGAAGCACGGGCGAAAGGAGAAAACGAA
GCCGCGTTTGCCGCCCGTATTCACTCGCTTTTTACCGTACCGAAGACCTGTATTCTGGGCTACAACAATGTGCGTTTCGA
CGACGAAGTCACACGCAACGTTTTTTATCGTAATTTCTACGATCCTTACGCCTGGAGCTGGCAGCATGATAACTCGCGCT
GGGATTTGCTGGATGTTATGCGTGCCTGTTATGCCCTGCGCCCGGAAGGAATAAACTGGCCTGAAAATGATGACGGTCTA
CCGAGCTTTCGCCTTGAGCATTTAACCAAAGCGAATGGTATTGAACATAGCAACGCCCACGATGCGATGGCTGATGTGTA
CGCCACTATTGCGATGGCAAAGCTGGTAAAAACGCGTCAGCCACGCCTGTTTGATTATCTCTTTACCCATCGCAATAAAC
ACAAACTGATGGCGTTGATTGATGTTCCGCAGATGAAACCCCTGGTTCACGTTTCCGGAATGTTTGGGGCATGGCGCGGC
AATACCAGCTGGGTGGCACCGCTGGCGTGGCATCCTGAAAATCGCAATGCCGTAATTATGGTGGATTTGGCAGGAGACAT
TTCGCCATTACTGGAACTGGATAGCGACACATTGCGCGAGCGTTTATATACCGCAAAAACCGATCTTGGCGATAACGCCG
CCGTTCCGGTTAAGCTGGTGCATATCAATAAATGTCCGGTGCTGGCCCAGGCGAATACGCTACGCCCGGAAGATGCCGAC
CGACTGGGAATTAATCGTCAGCATTGCCTCGATAACCTGAGAATTCTGCGTGAAAATCCGCAAGTGCGCGAAAAAGTGGT
GGCGATATTCGCGGAAGCCGAACCGTTTACGCCTTCAGATAACGTGGATGCACAGCTTTATAACGGCTTTTTCAGTGACG
CCGATCGTGCAGCAATGAAAATTGTGCTGGAAACCGAGCCACGTAATTTACCGGCACTGGATATCACTTTTGTTGATAAA
CGAATTGAAAAACTGTTGTTCAATTATCGGGCGCGCAACTTCCCGGGGACGCTGGATTATGCCGAGCAGCAACGCTGGCT
GGAGCACCGCCGCCAGGTCTTCACGCCAGAGTTTTTGCAGGGCTATGCTGATGAATTGCAGATGCTGGCACAACAATATG
CCGATGACAAAGAGAAAGTGGCGCTGTTAAAAGCGCTTTGGCAGTACGCGGAAGAGATTGTCTAA

Domains

Predicted by InterProScan.

Exonuclease C-terminal (ExoI_SH3)

(211-471)

Exonuclease (RNase_T)

(12-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  sbcB Vibrio cholerae O1 biovar El Tor strain E7946

59.149

99.156

0.586