Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CRN81_RS13495 Genome accession   NZ_CP024028
Coordinates   2958082..2959443 (-) Length   453 a.a.
NCBI ID   WP_099427298.1    Uniprot ID   -
Organism   Chromobacterium violaceum strain CV1192     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2953082..2964443
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN81_RS13465 (CRN81_13455) - 2953909..2954877 (+) 969 WP_099427295.1 hypothetical protein -
  CRN81_RS13470 (CRN81_13460) - 2954906..2955697 (-) 792 WP_011136516.1 cystine ABC transporter substrate-binding protein -
  CRN81_RS21475 - 2955694..2955819 (-) 126 WP_254894010.1 hypothetical protein -
  CRN81_RS13475 (CRN81_13465) - 2955953..2956306 (+) 354 WP_011136517.1 DUF2325 domain-containing protein -
  CRN81_RS13480 (CRN81_13470) - 2956352..2957257 (-) 906 WP_157760292.1 hypothetical protein -
  CRN81_RS13485 (CRN81_13475) - 2957321..2957518 (-) 198 WP_099427297.1 zf-HC2 domain-containing protein -
  CRN81_RS13490 (CRN81_13480) - 2957515..2958078 (-) 564 WP_011136520.1 sigma-70 family RNA polymerase sigma factor -
  CRN81_RS13495 (CRN81_13485) radA 2958082..2959443 (-) 1362 WP_099427298.1 DNA repair protein RadA Machinery gene
  CRN81_RS13500 (CRN81_13490) - 2959583..2960116 (-) 534 WP_011136522.1 hypothetical protein -
  CRN81_RS13505 (CRN81_13495) - 2960131..2960892 (-) 762 WP_099427299.1 SDR family oxidoreductase -
  CRN81_RS13510 (CRN81_13500) hpnE 2960889..2962160 (-) 1272 WP_232514871.1 hydroxysqualene dehydroxylase HpnE -
  CRN81_RS13515 (CRN81_13505) hpnD 2962157..2963002 (-) 846 WP_043615322.1 presqualene diphosphate synthase HpnD -
  CRN81_RS13520 (CRN81_13510) hpnC 2963073..2963885 (-) 813 WP_099427300.1 squalene synthase HpnC -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 48802.06 Da        Isoelectric Point: 6.5998

>NTDB_id=251302 CRN81_RS13495 WP_099427298.1 2958082..2959443(-) (radA) [Chromobacterium violaceum strain CV1192]
MAKNKTVFSCTECGGQSPKWQGQCPHCSAWNTLVEAVAAPAAASPRFQSWAANVTKVQKLSEVQTEETPRDPSGIDELDR
VLGGGIVRGAVVLIGGDPGIGKSTLLLQALSQIGQNRKVLYVSGEESAQQIALRASRLALDTSSVDLLAEICIENILETL
KREQPEVVVIDSIQTLYTEQVTSAPGSVSQVRECAAQLTRMAKQSGITVLLVGHVTKEGSLAGPRVLEHMVDTVLYFEGD
SHSSYRMIRAIKNRFGAVNELGVFAMTDRGLKGVSNPSAIFLSSYRDDVAGSCVLVTQEGTRPLLVEIQALVDDCHGFQP
KRLTVGLEQNRLSMLLAVLHRHGGVACFDQDVFLNAVGGVKINEPAADLAIILAMVSSLRNKALPEKLVVFGEVGLAGEV
RPVTRGQERLKEAAKLGFTRAIVPTANKPRQEIEGLKVLAVDRLDQAVEYCRE

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=251302 CRN81_RS13495 WP_099427298.1 2958082..2959443(-) (radA) [Chromobacterium violaceum strain CV1192]
ATGGCCAAGAACAAAACCGTATTCAGCTGCACCGAGTGCGGCGGACAGTCGCCCAAGTGGCAGGGCCAGTGCCCGCACTG
CAGCGCCTGGAACACGCTGGTGGAGGCGGTGGCCGCGCCGGCCGCCGCCAGTCCCCGCTTCCAGTCCTGGGCGGCCAACG
TCACCAAGGTGCAGAAACTGTCCGAGGTGCAGACCGAGGAAACCCCGCGCGATCCGTCCGGCATCGACGAACTGGACCGC
GTGCTGGGCGGCGGCATCGTGCGCGGCGCGGTGGTGCTGATCGGCGGCGACCCCGGCATCGGCAAGTCCACGCTGCTGTT
GCAGGCGCTGTCGCAGATCGGCCAGAACCGCAAGGTGCTCTACGTCTCCGGCGAGGAATCGGCGCAGCAGATCGCCTTGC
GCGCCTCGCGGCTGGCGCTGGATACCAGCAGCGTCGATCTGCTGGCCGAGATCTGCATCGAGAACATTCTGGAAACGCTG
AAGCGAGAGCAGCCGGAAGTAGTGGTGATCGACTCGATCCAGACGCTTTACACCGAGCAGGTGACGTCGGCGCCGGGCTC
GGTGTCGCAGGTGCGCGAGTGCGCGGCGCAGCTGACGCGGATGGCCAAGCAGAGCGGCATCACCGTGCTGCTGGTCGGCC
ACGTGACCAAGGAAGGTTCGCTGGCCGGTCCGCGCGTGCTGGAGCACATGGTGGACACCGTGCTGTATTTCGAGGGCGAC
TCCCACTCCAGCTACCGCATGATACGCGCGATCAAGAACCGCTTCGGCGCGGTCAACGAGCTGGGCGTGTTCGCGATGAC
CGACCGCGGCCTGAAGGGCGTGTCCAATCCGTCGGCCATCTTCCTTTCCTCGTATCGCGACGACGTCGCCGGCTCCTGCG
TGCTGGTGACGCAGGAAGGCACGCGGCCGCTGCTGGTGGAAATCCAGGCCTTGGTCGACGACTGCCACGGCTTCCAGCCC
AAGCGTCTCACCGTCGGCCTGGAGCAGAACCGCCTGTCCATGCTGCTGGCGGTGCTGCACCGCCACGGCGGCGTCGCCTG
TTTCGACCAGGACGTGTTCCTCAACGCGGTGGGCGGCGTCAAGATCAACGAGCCGGCCGCCGACCTCGCCATCATCCTGG
CCATGGTTTCGTCGCTGCGCAACAAGGCGCTGCCGGAAAAGCTGGTGGTGTTCGGCGAGGTGGGCCTGGCCGGCGAGGTG
CGGCCGGTGACGCGGGGCCAGGAACGCTTGAAGGAGGCGGCCAAGCTGGGCTTCACCCGCGCCATCGTGCCCACCGCCAA
CAAGCCGCGGCAGGAAATCGAGGGCCTGAAGGTCTTGGCCGTGGACCGCCTGGATCAGGCGGTGGAATACTGCAGGGAGT
AG

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(67-219)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

50.439

100

0.508
  radA Streptococcus mitis NCTC 12261

48.451

99.779

0.483
  radA Streptococcus mitis SK321

48.451

99.779

0.483
  radA Streptococcus pneumoniae Rx1

50.236

93.598

0.47
  radA Streptococcus pneumoniae D39

50.236

93.598

0.47
  radA Streptococcus pneumoniae R6

50.236

93.598

0.47
  radA Streptococcus pneumoniae TIGR4

50.236

93.598

0.47

Multiple sequence alignment