Detailed information NTG0025124

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: NMUL_RS14255
• Genome accession: NC_007614
• Coordinates: 3143555..3144541 (+)
• Length: 328 a.a.
• NCBI ID: WP_011382017.1
• Uniprot ID: Q2Y5E6
• Organism: Nitrosospira multiformis ATCC 25196
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3138555..3149541

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NMUL_RS14230 (Nmul_A2733)	-	3139364..3139624 (-)	261	WP_011382012.1	YfhL family 4Fe-4S dicluster ferredoxin	-
NMUL_RS14235 (Nmul_A2734)	coaD	3139627..3140124 (-)	498	WP_011382013.1	pantetheine-phosphate adenylyltransferase	-
NMUL_RS14240 (Nmul_A2735)	rsmD	3140125..3140664 (-)	540	WP_011382014.1	16S rRNA (guanine(966)-N(2))-methyltransferase RsmD	-
NMUL_RS14245 (Nmul_A2736)	-	3140727..3142037 (-)	1311	WP_011382015.1	M16 family metallopeptidase	-
NMUL_RS14250 (Nmul_A2737)	-	3142054..3143451 (-)	1398	WP_011382016.1	M16 family metallopeptidase	-
NMUL_RS14255 (Nmul_A2738)	pilA	3143555..3144541 (+)	987	WP_011382017.1	signal recognition particle-docking protein FtsY	Machinery gene
NMUL_RS14260 (Nmul_A2739)	ftsE	3144615..3145268 (+)	654	WP_011382018.1	cell division ATP-binding protein FtsE	-
NMUL_RS14265 (Nmul_A2740)	ftsX	3145265..3146173 (+)	909	WP_011382019.1	permease-like cell division protein FtsX	-
NMUL_RS14270 (Nmul_A2741)	rpoH	3146280..3147125 (+)	846	WP_011382020.1	RNA polymerase sigma factor RpoH	-
NMUL_RS14275 (Nmul_A2742)	-	3147187..3147438 (-)	252	WP_011382021.1	BolA family protein	-
NMUL_RS14280 (Nmul_A2743)	-	3147540..3148295 (-)	756	WP_011382022.1	ABC transporter permease	-
NMUL_RS14285 (Nmul_A2744)	-	3148292..3149221 (-)	930	WP_011382023.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 328 a.a.
• Molecular weight: 35314.29 Da
• Isoelectric Point: 6.9984

>NTDB_id=25124 NMUL_RS14255 WP_011382017.1 3143555..3144541(+) (pilA) [Nitrosospira multiformis ATCC 25196]
MASFFKSKNDSTTSEPPNSNWGVRVRQGLSRTRNNLGKRLSGLFGGGAIDEDLYEEMETILLTADAGVSATTWLLEELRR
KVKRNGLTDASQLKEALQEALVDLLEPLAQPLDTSAHHPFIIMLTGVNGAGKTTSIGKLAHYYQLQGKSVLLAAGDTFRA
AAREQLMAWGERNRVAVIAQESSDQQRGDPAAVIFDAVNAAKARGIDIVLADTAGRLATQLHLMEEIKKVKRVIAKTEPG
APHETLLVLDANTGQNAVNQVQAFDDALGLTGLIVTKLDGTAKGGVLAAIARQRQQKVPLPVRFIGVGEGLDDLRPFVAR
EFVGALFD

Nucleotide

• Length: 987 bp

>NTDB_id=25124 NMUL_RS14255 WP_011382017.1 3143555..3144541(+) (pilA) [Nitrosospira multiformis ATCC 25196]
ATGGCAAGTTTCTTCAAATCCAAAAATGATTCCACGACGAGCGAACCTCCGAATTCGAATTGGGGGGTGAGGGTTCGGCA
AGGCCTGTCCCGCACGCGTAACAACCTTGGCAAGCGCCTTTCGGGTCTCTTCGGTGGCGGAGCAATCGATGAAGATCTCT
ACGAGGAGATGGAAACCATATTGCTCACGGCGGATGCGGGTGTCAGTGCCACCACCTGGCTGCTGGAGGAGTTGCGCAGA
AAAGTCAAACGTAACGGCTTGACCGATGCGTCGCAGCTCAAAGAGGCGTTGCAGGAGGCGCTGGTCGACCTGCTGGAACC
GCTTGCGCAACCCCTCGATACCAGCGCCCACCACCCTTTCATCATCATGCTGACCGGGGTGAACGGTGCCGGAAAAACCA
CTTCCATTGGAAAACTTGCGCATTATTATCAACTGCAGGGCAAGTCCGTGCTGCTCGCCGCGGGCGATACCTTCCGTGCC
GCAGCGCGCGAGCAGCTGATGGCGTGGGGGGAGCGCAACCGCGTCGCGGTGATCGCGCAGGAAAGCAGCGATCAACAAAG
AGGTGATCCGGCGGCGGTCATATTCGATGCCGTCAACGCGGCGAAAGCGCGGGGCATCGATATCGTGCTGGCGGACACTG
CCGGAAGGCTGGCCACGCAACTCCACCTCATGGAGGAAATAAAAAAGGTGAAGCGCGTTATTGCCAAAACCGAGCCCGGG
GCGCCTCATGAAACGTTACTGGTTCTGGATGCGAATACCGGGCAGAATGCAGTCAATCAGGTACAGGCATTCGACGATGC
CCTTGGCCTGACAGGTCTCATTGTCACCAAGCTGGATGGAACCGCAAAAGGAGGCGTGCTTGCCGCCATCGCCCGCCAAC
GCCAGCAAAAAGTCCCGTTGCCTGTCCGGTTTATCGGTGTAGGTGAGGGTCTGGACGATTTGAGGCCTTTCGTCGCACGC
GAATTTGTCGGAGCGTTGTTTGATTGA

Domains

Predicted by InterproScan.

• SRP54-type protein, helical bundle domain (SRP54_N) (36-101)

• SRP54-type protein, GTPase domain (SRP54) (119-327)

3D structure

• AlphaFold DB: Q2Y5E6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Neisseria gonorrhoeae MS11	66.877	96.646	0.646