Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BS636_RS02950 Genome accession   NZ_CP024011
Coordinates   604870..605661 (-) Length   263 a.a.
NCBI ID   WP_213064231.1    Uniprot ID   -
Organism   Acinetobacter sp. LoGeW2-3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 599870..610661
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BS636_RS02930 (BS636_02930) - 600915..601613 (-) 699 WP_099337436.1 YfgM family protein -
  BS636_RS02935 (BS636_02935) hisS 601644..602939 (-) 1296 WP_099337437.1 histidine--tRNA ligase -
  BS636_RS02940 (BS636_02940) ispG 602943..604064 (-) 1122 WP_099337438.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
  BS636_RS02945 (BS636_02945) - 604088..604879 (-) 792 WP_099337439.1 helix-turn-helix domain-containing protein -
  BS636_RS02950 (BS636_02950) pilF 604870..605661 (-) 792 WP_213064231.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  BS636_RS02955 (BS636_02955) rlmN 605685..606920 (-) 1236 WP_099337441.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  BS636_RS02960 (BS636_02960) ndk 607042..607473 (-) 432 WP_004782097.1 nucleoside-diphosphate kinase -
  BS636_RS02965 (BS636_02965) iscX 607583..607783 (-) 201 WP_005232138.1 Fe-S cluster assembly protein IscX -
  BS636_RS02970 (BS636_02970) - 607872..608423 (-) 552 WP_099337442.1 phosphatase PAP2 family protein -
  BS636_RS02975 (BS636_02975) - 608494..608904 (-) 411 WP_099337443.1 hypothetical protein -

Sequence

Protein


Download         Length: 263 a.a.        Molecular weight: 29744.74 Da        Isoelectric Point: 9.2428

>NTDB_id=251122 BS636_RS02950 WP_213064231.1 604870..605661(-) (pilF) [Acinetobacter sp. LoGeW2-3]
MTSKIAFISIVCVSIALSACQTAEFGKKDPKKATQVRTQLAVEYLKTGDLDSAKRALDQALESNSRDAQANMLMGVLLQQ
EGSKANLDKAEGYFKKAISAEPKNAQARNNYGTYLYQLERYNDAIEQFAIAGSTLGYDQRYRALENMGRIYLKQGDLTNA
EKSFKQALQANRDSYVSMLELTEIFYLNQKFPAASNMYQQFVRSVGQKNQGARALWIGIRTARAEGDQMDMQVLVNQLRA
LFPESPEYQRYLQLQYSTEAVWK

Nucleotide


Download         Length: 792 bp        

>NTDB_id=251122 BS636_RS02950 WP_213064231.1 604870..605661(-) (pilF) [Acinetobacter sp. LoGeW2-3]
ATGACATCTAAAATTGCTTTTATTTCAATAGTGTGTGTTTCTATAGCCCTATCTGCCTGTCAAACGGCAGAATTCGGTAA
AAAGGATCCTAAGAAAGCGACTCAGGTGCGTACCCAATTGGCGGTTGAATATCTGAAAACAGGTGATCTGGATTCAGCCA
AGCGTGCGCTGGATCAGGCACTTGAAAGTAATTCACGTGATGCCCAGGCTAATATGCTGATGGGCGTATTGCTGCAACAG
GAAGGCAGTAAGGCTAATTTAGATAAAGCTGAAGGCTATTTTAAAAAAGCGATTTCCGCTGAACCTAAAAATGCACAGGC
ACGTAACAATTACGGCACCTATTTATATCAACTTGAACGTTATAATGACGCGATTGAACAATTTGCAATTGCAGGTTCTA
CGCTGGGCTATGATCAGCGTTACCGTGCGCTTGAAAATATGGGGCGGATCTATCTGAAACAGGGTGATCTGACAAATGCT
GAAAAATCATTCAAACAAGCCCTGCAAGCCAATCGCGATTCTTATGTTTCAATGTTAGAGTTGACAGAGATTTTTTATCT
GAATCAGAAGTTCCCAGCAGCGTCGAATATGTATCAACAATTTGTACGTTCAGTGGGGCAGAAAAACCAAGGTGCTCGTG
CGCTTTGGATCGGCATTCGTACTGCGCGTGCCGAGGGTGATCAAATGGATATGCAAGTATTGGTGAATCAATTACGCGCA
TTATTCCCTGAAAGCCCGGAATACCAACGTTATTTGCAATTACAGTACAGTACTGAGGCCGTATGGAAGTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_8)

(142-173)

Tetratricopeptide repeat (TPR_16)

(39-81)

Tetratricopeptide repeat (TPR_17)

(94-126)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Acinetobacter baumannii D1279779

75.379

100

0.757
  pilF Acinetobacter baylyi ADP1

67.954

98.479

0.669

Multiple sequence alignment