Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   CRN72_RS08275 Genome accession   NZ_CP023972
Coordinates   1717871..1719205 (+) Length   444 a.a.
NCBI ID   WP_096743657.1    Uniprot ID   -
Organism   Pasteurella multocida strain FDAARGOS_385     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1712871..1724205
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRN72_RS08250 (CRN72_08250) - 1712947..1715508 (-) 2562 WP_005754757.1 penicillin-binding protein 1A -
  CRN72_RS08255 (CRN72_08255) - 1715644..1716417 (+) 774 WP_096743654.1 pilus assembly protein PilM -
  CRN72_RS08260 (CRN72_08260) - 1716457..1716972 (+) 516 WP_096743655.1 competence protein ComB -
  CRN72_RS08265 (CRN72_08265) - 1716972..1717496 (+) 525 WP_096743656.1 ATPase -
  CRN72_RS08270 (CRN72_08270) - 1717499..1717861 (+) 363 WP_083003380.1 pilus assembly protein PilP -
  CRN72_RS08275 (CRN72_08275) comE 1717871..1719205 (+) 1335 WP_096743657.1 type IV pilus secretin PilQ Machinery gene
  CRN72_RS08280 (CRN72_08280) aroK 1719388..1719915 (+) 528 WP_005717623.1 shikimate kinase AroK -
  CRN72_RS08285 (CRN72_08285) aroB 1719932..1721020 (+) 1089 WP_083003375.1 3-dehydroquinate synthase -
  CRN72_RS08290 (CRN72_08290) - 1721024..1721929 (+) 906 WP_083003372.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  CRN72_RS08295 (CRN72_08295) - 1721977..1722282 (-) 306 WP_083003370.1 DUF721 domain-containing protein -
  CRN72_RS08300 (CRN72_08300) secM 1722375..1722686 (+) 312 WP_005723689.1 secA translation cis-regulator SecM -

Sequence

Protein


Download         Length: 444 a.a.        Molecular weight: 49236.56 Da        Isoelectric Point: 7.1841

>NTDB_id=251073 CRN72_RS08275 WP_096743657.1 1717871..1719205(+) (comE) [Pasteurella multocida strain FDAARGOS_385]
MWRAFRKLSFVYFLCGVAYVGSSQAQDAEHFYLRLKQAPLVEMLQYLALRQHQDLLIDDHLEGTLSLQMKKTTFEKCLQS
IAKMKQLEFHQEGKSYYLTSSSGVAANDTHHPTSLMTSSIKLHFAKAADVVQSLTSGQGSLLSVGGSLSFDERTNLLLIQ
DEPQSIQRIKALVAEMDKPIEQIAIEARIVTMTDESLQELGVRWGLFQATEQAHTIAGSLAANGFSNIENQLNVNFTTNS
APVGSIALQLAKINGRLLDLELTALEREKHIEIIASPRLLTTNKKSASIKQGTEIPYVMKRGKDKSESVEFREAVLGLDV
TPHISKDNTILLDLLITQNTLGAPVVYDKGEIVSIDKQEINTQVVAQDGETIVLGGVFHDTMTKGVNKVPLLGDLPLLKH
VFSQETERHQKRELVIFVTPHIIKPTQSLPEQKTTGVKKSAKSR

Nucleotide


Download         Length: 1335 bp        

>NTDB_id=251073 CRN72_RS08275 WP_096743657.1 1717871..1719205(+) (comE) [Pasteurella multocida strain FDAARGOS_385]
ATGTGGCGAGCATTCAGAAAACTATCTTTTGTGTACTTTTTATGTGGGGTTGCTTATGTTGGAAGTAGTCAAGCACAAGA
CGCAGAACATTTTTATTTACGTTTAAAACAAGCACCTTTAGTCGAAATGTTACAGTATTTAGCATTGCGACAACATCAGG
ATTTGTTAATCGATGATCATTTAGAGGGCACATTATCATTACAGATGAAAAAGACAACCTTTGAGAAATGTTTACAGTCG
ATTGCAAAAATGAAACAACTGGAATTCCATCAAGAAGGGAAATCCTATTATTTAACTTCCTCTTCAGGTGTTGCAGCAAA
CGATACTCATCATCCTACGTCATTGATGACATCTTCAATAAAATTGCATTTTGCCAAAGCCGCAGATGTAGTGCAATCTT
TAACTTCAGGGCAGGGAAGTTTACTTTCTGTCGGGGGGAGTTTGAGTTTTGATGAGCGGACTAATTTACTGCTGATTCAG
GATGAACCGCAATCAATACAGCGTATTAAAGCATTAGTAGCAGAAATGGATAAACCCATTGAACAAATTGCGATCGAAGC
CAGGATTGTGACGATGACAGACGAAAGTTTGCAGGAACTTGGTGTAAGATGGGGGCTATTTCAAGCAACAGAACAGGCAC
ATACTATTGCGGGGAGTTTAGCCGCGAACGGCTTTTCGAATATAGAAAACCAATTAAATGTGAATTTTACGACCAATAGT
GCACCTGTTGGTTCCATCGCCTTACAGTTGGCGAAAATAAATGGTCGATTATTAGACTTGGAATTAACTGCCTTAGAGCG
AGAAAAGCATATTGAGATTATTGCGAGTCCTCGTTTATTAACAACGAATAAAAAAAGTGCCAGTATCAAACAAGGGACGG
AAATTCCTTATGTGATGAAACGGGGAAAAGATAAAAGCGAATCGGTAGAATTTCGAGAAGCTGTATTAGGTTTAGATGTG
ACACCGCATATCTCAAAAGACAACACGATTTTATTAGATTTATTGATTACACAAAATACATTAGGTGCACCGGTAGTGTA
TGATAAAGGCGAAATTGTTTCGATCGATAAACAGGAAATCAATACTCAAGTCGTCGCTCAAGATGGTGAAACCATCGTTT
TAGGTGGGGTGTTTCATGATACGATGACAAAGGGAGTCAATAAAGTACCACTACTAGGGGATTTGCCTTTGCTTAAACAT
GTGTTTAGCCAGGAAACTGAACGTCATCAAAAGCGAGAATTAGTGATTTTTGTCACGCCTCATATTATCAAACCGACCCA
AAGTTTGCCTGAACAAAAAACAACGGGAGTTAAAAAATCTGCAAAATCGAGGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(119-182)

Bacterial type II and III secretion system protein (Secretin)

(264-424)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

60.899

100

0.61
  comE Haemophilus influenzae 86-028NP

60.225

100

0.604
  comE Glaesserella parasuis strain SC1401

49.173

95.27

0.468
  pilQ Vibrio campbellii strain DS40M4

39.762

94.595

0.376
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.049

92.793

0.372
  pilQ Vibrio cholerae strain A1552

40.049

92.793

0.372

Multiple sequence alignment