Detailed information    

insolico Bioinformatically predicted

Overview

Name   waaF   Type   Regulator
Locus tag   CRM97_RS08195 Genome accession   NZ_CP023866
Coordinates   1542789..1543739 (+) Length   316 a.a.
NCBI ID   WP_011049891.1    Uniprot ID   -
Organism   Campylobacter jejuni strain FDAARGOS_421     
Function   repress natural transformation (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1528837..1548551 1542789..1543739 within 0


Gene organization within MGE regions


Location: 1528837..1548551
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRM97_RS08130 (CRM97_08130) pglI 1528837..1529766 (-) 930 WP_002867212.1 GalNAc(5)-diNAcBac-PP-undecaprenol beta-1,3-glucosyltransferase -
  CRM97_RS08135 (CRM97_08135) pglH 1529759..1530838 (-) 1080 WP_002859237.1 GalNAc-alpha-(1->4)-GalNAc-alpha-(1->3)- diNAcBac-PP-undecaprenol alpha-1,4-N-acetyl-D-galactosaminyltransferase -
  CRM97_RS08140 (CRM97_08140) pglK 1530835..1532529 (-) 1695 WP_002867213.1 ABC-type lipopolysaccharide transporter PglK -
  CRM97_RS08145 (CRM97_08145) galE 1532523..1533509 (-) 987 WP_002867214.1 UDP-glucose 4-epimerase GalE -
  CRM97_RS08150 (CRM97_08150) - 1533568..1534362 (-) 795 WP_002860221.1 3'-5' exonuclease -
  CRM97_RS08155 (CRM97_08155) waaC 1534429..1535457 (+) 1029 WP_011049885.1 lipopolysaccharide heptosyltransferase I -
  CRM97_RS08160 (CRM97_08160) htrB 1535447..1536334 (+) 888 WP_002867215.1 lipid A biosynthesis lauroyl acyltransferase HtrB -
  CRM97_RS08165 (CRM97_08165) - 1536331..1537881 (+) 1551 WP_002867216.1 glycosyltransferase -
  CRM97_RS08170 (CRM97_08170) - 1537850..1538737 (-) 888 WP_011049886.1 glycosyltransferase family 2 protein -
  CRM97_RS08175 (CRM97_08175) - 1538730..1539938 (-) 1209 WP_011049887.1 glycosyltransferase family 2 protein -
  CRM97_RS08180 (CRM97_08180) - 1540016..1540987 (-) 972 WP_011049888.1 glycosyltransferase -
  CRM97_RS08185 (CRM97_08185) - 1540980..1541867 (-) 888 WP_011049889.1 glycosyltransferase -
  CRM97_RS08190 (CRM97_08190) waaV 1541921..1542730 (-) 810 WP_011049890.1 glycosyltransferase family 2 protein -
  CRM97_RS08195 (CRM97_08195) waaF 1542789..1543739 (+) 951 WP_011049891.1 lipopolysaccharide heptosyltransferase II Regulator
  CRM97_RS08200 (CRM97_08200) - 1543725..1544489 (-) 765 WP_002884483.1 glycosyltransferase family 25 protein -
  CRM97_RS08205 (CRM97_08205) gmhA 1544489..1545049 (-) 561 WP_002869619.1 D-sedoheptulose 7-phosphate isomerase -
  CRM97_RS08210 (CRM97_08210) rfaE1 1545046..1546431 (-) 1386 WP_002867399.1 D-glycero-beta-D-manno-heptose-7-phosphate kinase -
  CRM97_RS08215 (CRM97_08215) rfaD 1546424..1547377 (-) 954 WP_002867398.1 ADP-glyceromanno-heptose 6-epimerase -
  CRM97_RS08220 (CRM97_08220) gmhB 1547378..1547939 (-) 562 Protein_1592 D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase -
  CRM97_RS08225 (CRM97_08225) - 1548020..1548322 (+) 303 WP_002852762.1 cytochrome c -
  CRM97_RS08230 (CRM97_08230) ccoS 1548345..1548551 (-) 207 WP_002852798.1 cbb3-type cytochrome oxidase assembly protein CcoS -

Sequence

Protein


Download         Length: 316 a.a.        Molecular weight: 36213.76 Da        Isoelectric Point: 10.2878

>NTDB_id=250720 CRM97_RS08195 WP_011049891.1 1542789..1543739(+) (waaF) [Campylobacter jejuni strain FDAARGOS_421]
MKIFIHLPTWLGDAVMASPALYVIKEHFKNAQFILYGSLVSTALFKEFPNSKIIIENKQSRYKQALSLRKELGKIDFSFA
FRSAFSSKIILHILKTKQRYFFDKNKHKEEHQVLKYLYFIENSLHIKAHLKDLKLPFKLKFQSPLVLKNGKKILGLNPGA
SFGSAKRWDASYFAKVALNFSQSHEILIFGAGKAEQELCNEIYQILKEQNIKVKNLCNKTTIKTLCQNIAFCDLFITNDS
GPMHISAVYKVKTVAIFGPTKFTQTSPWQNENATLVHLNLACMPCMQKTCPLKHHKCMKDLKPEVIVKAIQNLTNI

Nucleotide


Download         Length: 951 bp        

>NTDB_id=250720 CRM97_RS08195 WP_011049891.1 1542789..1543739(+) (waaF) [Campylobacter jejuni strain FDAARGOS_421]
ATGAAAATTTTTATACACCTTCCCACTTGGCTAGGCGATGCGGTGATGGCTTCACCTGCTTTATACGTTATAAAAGAACA
TTTTAAAAATGCCCAATTTATCCTTTATGGATCTTTGGTTTCCACAGCACTTTTTAAAGAATTTCCTAATTCTAAAATCA
TCATAGAAAATAAACAATCCCGCTACAAACAAGCCCTATCTTTACGCAAAGAACTTGGTAAGATCGATTTTAGCTTTGCT
TTTAGATCTGCGTTTTCTTCTAAGATTATCTTACATATTCTTAAAACAAAACAAAGATATTTTTTTGACAAAAACAAGCA
CAAAGAAGAACATCAAGTTTTAAAATACCTTTATTTTATAGAAAACTCACTTCACATAAAAGCTCACCTTAAAGACTTAA
AACTTCCCTTTAAGCTAAAATTTCAAAGCCCTCTTGTCTTAAAAAATGGCAAAAAAATTCTAGGACTCAATCCTGGTGCA
AGCTTTGGAAGTGCAAAAAGATGGGATGCGAGTTATTTTGCTAAAGTGGCTTTAAATTTCAGCCAAAGTCATGAGATTTT
AATCTTTGGCGCAGGAAAAGCCGAACAAGAACTTTGTAATGAAATTTATCAAATTTTAAAAGAACAAAACATAAAAGTAA
AAAATCTTTGCAATAAAACTACCATCAAAACCCTTTGTCAAAATATCGCTTTTTGCGATCTTTTCATCACAAATGACAGT
GGCCCTATGCACATAAGTGCAGTTTATAAGGTAAAAACCGTGGCTATTTTTGGCCCTACAAAATTTACTCAAACCTCACC
TTGGCAAAATGAAAACGCAACATTAGTGCATTTAAATCTAGCTTGTATGCCTTGTATGCAAAAAACCTGCCCTTTAAAAC
ACCACAAATGCATGAAAGATTTAAAGCCTGAAGTGATTGTAAAAGCGATTCAAAATTTAACTAATATCTAA

Domains

Predicted by InterproScan.

Glycosyltransferase family 9 (heptosyltransferase) (Glyco_transf_9)

(96-294)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  waaF Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819

92.652

99.051

0.918

Multiple sequence alignment