Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CRH11_RS14150 Genome accession   NZ_CP023859
Coordinates   2812636..2813070 (+) Length   144 a.a.
NCBI ID   WP_015417516.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2807636..2818070
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRH11_RS14145 (CRH11_14140) - 2811136..2812347 (-) 1212 WP_094031922.1 cytochrome P450 family protein -
  CRH11_RS14150 (CRH11_14145) nucA/comI 2812636..2813070 (+) 435 WP_015417516.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CRH11_RS14155 (CRH11_14150) - 2813130..2813885 (+) 756 WP_094031726.1 YoaK family protein -
  CRH11_RS14160 (CRH11_14155) - 2813919..2814281 (-) 363 WP_007410383.1 hypothetical protein -
  CRH11_RS14165 (CRH11_14160) - 2814475..2815803 (-) 1329 WP_015239896.1 S8 family peptidase -
  CRH11_RS14170 (CRH11_14165) - 2815982..2816215 (+) 234 WP_015239897.1 hypothetical protein -
  CRH11_RS14175 (CRH11_14170) - 2816471..2817178 (+) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  CRH11_RS14180 (CRH11_14175) - 2817238..2817690 (+) 453 WP_039251441.1 OsmC family protein -
  CRH11_RS14185 (CRH11_14180) - 2817704..2818057 (-) 354 WP_094031727.1 DMT family transporter -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15514.44 Da        Isoelectric Point: 7.2418

>NTDB_id=250573 CRH11_RS14150 WP_015417516.1 2812636..2813070(+) (nucA/comI) [Bacillus velezensis strain L-1]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADVRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=250573 CRH11_RS14150 WP_015417516.1 2812636..2813070(+) (nucA/comI) [Bacillus velezensis strain L-1]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGTATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCAGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATGTCAGATATGTAACCCCGTCGGATAACCGCGGGGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

57.258

86.111

0.493

Multiple sequence alignment