Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CRH11_RS07080 Genome accession   NZ_CP023859
Coordinates   1347630..1348067 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain L-1     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1342630..1353067
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRH11_RS07055 (CRH11_07050) - 1343649..1344785 (+) 1137 WP_094031490.1 zinc-dependent alcohol dehydrogenase -
  CRH11_RS07060 (CRH11_07055) - 1344800..1345234 (+) 435 WP_094031491.1 RDD family protein -
  CRH11_RS07065 (CRH11_07060) - 1345352..1345630 (+) 279 WP_263612330.1 YckD family protein -
  CRH11_RS07070 (CRH11_07065) - 1345734..1347170 (+) 1437 WP_076425807.1 glycoside hydrolase family 1 protein -
  CRH11_RS07075 (CRH11_07070) nin/comJ 1347211..1347609 (-) 399 WP_007609299.1 competence protein ComJ Regulator
  CRH11_RS07080 (CRH11_07075) nucA/comI 1347630..1348067 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CRH11_RS07085 (CRH11_07080) hxlB 1348426..1348983 (-) 558 WP_007409354.1 6-phospho-3-hexuloisomerase -
  CRH11_RS07090 (CRH11_07085) hxlA 1348980..1349615 (-) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  CRH11_RS07095 (CRH11_07090) - 1349847..1350209 (+) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=250545 CRH11_RS07080 WP_003156588.1 1347630..1348067(-) (nucA/comI) [Bacillus velezensis strain L-1]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=250545 CRH11_RS07080 WP_003156588.1 1347630..1348067(-) (nucA/comI) [Bacillus velezensis strain L-1]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAACGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCATGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATTAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment