Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CK911_RS18850 Genome accession   NZ_CP023817
Coordinates   4165363..4166802 (+) Length   479 a.a.
NCBI ID   WP_098971865.1    Uniprot ID   A0A291TN87
Organism   Aeromonas sp. CU5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4160363..4171802
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CK911_RS18820 (CK911_18820) mlaF 4160940..4161740 (-) 801 WP_042024519.1 phospholipid ABC transporter ATP-binding protein MlaF -
  CK911_RS18825 (CK911_18825) - 4161948..4162943 (+) 996 WP_042024520.1 KpsF/GutQ family sugar-phosphate isomerase -
  CK911_RS18830 (CK911_18830) kdsC 4162943..4163497 (+) 555 WP_042024521.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CK911_RS18835 (CK911_18835) lptC 4163494..4164054 (+) 561 WP_098971859.1 LPS export ABC transporter periplasmic protein LptC -
  CK911_RS18840 (CK911_18840) lptA 4164041..4164568 (+) 528 WP_042024523.1 lipopolysaccharide transport periplasmic protein LptA -
  CK911_RS18845 (CK911_18845) lptB 4164572..4165297 (+) 726 WP_098971863.1 LPS export ABC transporter ATP-binding protein -
  CK911_RS18850 (CK911_18850) rpoN/rpoN1 4165363..4166802 (+) 1440 WP_098971865.1 RNA polymerase factor sigma-54 Machinery gene
  CK911_RS18855 (CK911_18855) hpf 4166824..4167111 (+) 288 WP_042024526.1 ribosome hibernation promoting factor -
  CK911_RS18860 (CK911_18860) ptsN 4167114..4167560 (+) 447 WP_042024527.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CK911_RS18865 (CK911_18865) rapZ 4167607..4168473 (+) 867 WP_042024529.1 RNase adapter RapZ -
  CK911_RS18870 (CK911_18870) npr 4168494..4168766 (+) 273 WP_042024530.1 PTS phosphocarrier protein NPr -
  CK911_RS18875 (CK911_18875) - 4169074..4169505 (-) 432 Protein_3640 KDGP aldolase -
  CK911_RS18880 (CK911_18880) - 4169703..4171745 (-) 2043 WP_098971867.1 TonB-dependent siderophore receptor -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53705.73 Da        Isoelectric Point: 4.3635

>NTDB_id=250315 CK911_RS18850 WP_098971865.1 4165363..4166802(+) (rpoN/rpoN1) [Aeromonas sp. CU5]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALENNPLLEQEESEVQETSPEEPVDASQLDSSDAMAQEQMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVELED
ILQAVSSDALEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYDPQTPWLNEAKEVLLEHMDLLGNRDYRTLARK
TKLKESDLKSVLDLIQLLEPRPGNGIIQSDSQYVIPDVSVVKKGSKWVVELNPDSSPRIRVNETYAAMARNARSGTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVACCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLFIAPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=250315 CK911_RS18850 WP_098971865.1 4165363..4166802(+) (rpoN/rpoN1) [Aeromonas sp. CU5]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTTTGGAACTTCAGCAGGAAATTCAGCAAGCGCTCGAGAACAACCCCCTGCTGGAGCAGGAAGAGAGTGAAG
TACAAGAGACCAGCCCGGAAGAGCCTGTTGACGCCAGCCAGCTGGATTCCAGCGATGCCATGGCGCAGGAGCAGATGCCG
GACGAGTTGCCGGTAGATACCACTTGGGATGAAGTCTACTCTGCCGGGACCGCCCAGAGCGCTGGCCCTATCCACGATGG
GGAAGATAGCGTTTATCAGGGCGAGACTACCGAAAACCTGCAGGATTATCTGTTGTGGCAAATGCGGCTCACCCCGTTCA
GCGATGTGGATGCTGCCATCGCCCTCGCTATCATCGATGCTATCGACGAGTCCGGCTATTTGACCGTTGAGCTGGAGGAT
ATCTTGCAAGCGGTCAGTAGCGATGCGCTGGAGGTCGAGCTTGATGAGGTCGAAGCTGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCTGCCCGTTCGGTGCAGGAGTGTTTGTTGATCCAGCTGGGGCAGTATGACCCGCAGACTCCCT
GGCTCAACGAGGCCAAGGAGGTGCTCCTCGAACATATGGATCTGCTCGGTAATCGTGACTACCGAACCCTGGCGCGCAAG
ACCAAACTCAAAGAGAGCGACCTCAAGTCGGTGCTGGATCTGATCCAGCTGCTGGAGCCGCGCCCCGGCAATGGCATCAT
CCAGAGTGACTCCCAGTATGTGATCCCCGATGTCTCTGTGGTCAAGAAGGGTAGCAAGTGGGTCGTTGAGCTAAATCCTG
ATTCTTCCCCGCGTATCCGGGTCAACGAGACCTATGCTGCCATGGCCCGCAATGCGCGCAGTGGCACGGATACCCAATTT
ATTCGCTCCCATCTGCAAGAAGCCAAATGGTTCATCAAGAGCCTCGAGAGTCGCAATGATACCCTGCTGAAAGTAGCCTG
TTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAGGCGATGAAGCCGATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCATGAATCGACCATTTCCCGGGTGACAACCCAGAAATACATGCATACCCCAAGGGGCATATTTGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCAGAGAATCCTGCCAAGCCGTTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCTAAGTATCGCGAATCCTTGTTTATCGCACCTTCCAATCAGCGTAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A291TN87

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.793

100

0.489

Multiple sequence alignment