Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CP523_RS08850 Genome accession   NZ_CP023671
Coordinates   1978961..1980328 (+) Length   455 a.a.
NCBI ID   WP_066678101.1    Uniprot ID   A0A9N7JL02
Organism   Clostridium septicum strain DSM 7534     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1973961..1985328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CP523_RS08815 (CP523_08795) trxB 1973970..1974911 (+) 942 WP_066678087.1 thioredoxin-disulfide reductase -
  CP523_RS08820 (CP523_08800) - 1974913..1975140 (+) 228 WP_120140787.1 glutaredoxin domain-containing protein -
  CP523_RS08825 (CP523_08805) - 1975178..1976641 (+) 1464 WP_066678091.1 NADP-dependent glyceraldehyde-3-phosphate dehydrogenase -
  CP523_RS08830 (CP523_08810) - 1976681..1976908 (-) 228 WP_066678093.1 glutaredoxin family protein -
  CP523_RS08835 (CP523_08815) - 1977054..1977215 (-) 162 WP_162925972.1 hypothetical protein -
  CP523_RS08840 (CP523_08820) - 1977344..1978066 (+) 723 WP_066678096.1 GntR family transcriptional regulator -
  CP523_RS08845 (CP523_08825) nagB 1978063..1978794 (+) 732 WP_066678099.1 glucosamine-6-phosphate deaminase -
  CP523_RS08850 (CP523_08830) radA 1978961..1980328 (+) 1368 WP_066678101.1 DNA repair protein RadA Machinery gene
  CP523_RS08855 (CP523_08835) disA 1980345..1981409 (+) 1065 WP_066678104.1 DNA integrity scanning diadenylate cyclase DisA -
  CP523_RS08860 (CP523_08840) - 1981490..1981888 (-) 399 WP_066678106.1 DUF1573 domain-containing protein -
  CP523_RS08865 (CP523_08845) - 1982189..1983274 (+) 1086 WP_120140788.1 PIN/TRAM domain-containing protein -
  CP523_RS08870 (CP523_08850) ispD 1983291..1983965 (+) 675 WP_066678110.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 50418.34 Da        Isoelectric Point: 7.5817

>NTDB_id=249187 CP523_RS08850 WP_066678101.1 1978961..1980328(+) (radA) [Clostridium septicum strain DSM 7534]
MAKIKSIFVCQECGYESLKWLGKCPDCNKWNTMVEEVKNTQKEQKSKINTIKVLGSMPKNIQEIKSGEKERFNTGLKELN
RVLGGGLVKGSLTLISGDPGIGKSTLLLQTANNIAKNYGKVLYVSGEESEEQIKIRGDRLGVNENNLYVLSETNLDLIEG
YINELNPVFVIIDSIQTVYKEAVTSAPGSVSQVKECSNAIMRIGKSKNIPLFIVAHVTKQGELAGPRVLEHMVDTVLYFE
GERTEEFRVLRTMKNRFGTTSEIGVFEMVEEGLKEIYDPSRIFLEDTNFNQEGSAVIGLMEGTRPILVEMQALVSETNMH
MASRTAVGIDNQRLRLMLAVLEKKLKAPFFKYDVYVNVVGGLNLDGTTGDLGLALALLSSLKNTGFKLERAVIIGEVGLT
GEIRPIASCDRLIKEAEKMGFKNAIIPNRNKDKVTSNEINIIGVSNIREAINKIF

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=249187 CP523_RS08850 WP_066678101.1 1978961..1980328(+) (radA) [Clostridium septicum strain DSM 7534]
ATGGCTAAAATTAAATCCATATTTGTCTGTCAAGAGTGCGGGTATGAATCATTAAAGTGGTTGGGAAAATGTCCTGACTG
CAATAAATGGAATACCATGGTAGAAGAAGTCAAGAATACACAAAAAGAACAAAAATCAAAAATAAACACAATAAAGGTTT
TAGGTAGTATGCCTAAGAATATACAAGAGATAAAATCTGGAGAAAAGGAAAGATTTAATACTGGTTTAAAAGAGTTAAAT
AGAGTTTTAGGTGGAGGATTAGTTAAGGGATCACTTACTCTAATTTCAGGAGATCCTGGAATAGGAAAATCCACATTATT
ATTGCAGACAGCAAATAACATTGCTAAAAACTATGGCAAAGTACTATATGTTTCAGGAGAAGAATCAGAGGAGCAGATAA
AGATAAGAGGAGATAGGTTAGGAGTAAATGAAAATAATCTATATGTGCTATCAGAAACTAACTTAGACTTAATAGAAGGA
TATATAAATGAGTTAAATCCTGTTTTTGTAATAATAGACTCTATTCAGACTGTATATAAAGAAGCAGTAACTTCAGCACC
AGGTAGTGTCTCACAAGTAAAAGAATGTTCAAACGCCATAATGAGAATTGGAAAAAGCAAGAATATACCTTTATTTATAG
TAGCTCATGTTACAAAGCAAGGGGAACTTGCTGGACCTAGAGTTTTAGAACATATGGTTGATACGGTTCTGTATTTTGAA
GGAGAGAGAACGGAAGAGTTTAGGGTTCTAAGAACTATGAAAAATCGTTTTGGAACAACTAGTGAAATTGGTGTTTTTGA
AATGGTAGAAGAAGGTTTGAAGGAAATATACGATCCATCTAGAATATTCTTAGAAGATACTAACTTTAATCAAGAAGGAT
CTGCCGTAATAGGACTTATGGAAGGAACTAGACCCATACTTGTAGAAATGCAGGCTCTTGTAAGTGAAACTAATATGCAT
ATGGCTAGTAGAACTGCTGTTGGAATAGATAATCAAAGACTTAGATTAATGCTAGCTGTTTTAGAAAAAAAACTAAAAGC
ACCATTCTTTAAATATGATGTATATGTTAACGTAGTTGGTGGATTGAATTTAGATGGAACAACAGGAGATTTAGGATTGG
CTCTAGCTTTATTATCTAGTTTGAAAAATACTGGTTTTAAATTAGAAAGAGCAGTAATAATTGGAGAAGTAGGATTAACT
GGAGAAATAAGACCTATAGCATCATGTGACAGATTAATTAAAGAAGCAGAAAAAATGGGATTTAAAAATGCTATAATTCC
AAATAGAAATAAAGATAAAGTAACTAGTAATGAAATTAATATTATAGGAGTAAGTAATATAAGAGAAGCAATTAATAAGA
TATTCTAG

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(319-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

53.201

99.56

0.53
  radA Streptococcus pneumoniae Rx1

46.507

100

0.468
  radA Streptococcus pneumoniae D39

46.507

100

0.468
  radA Streptococcus pneumoniae R6

46.507

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.507

100

0.468
  radA Streptococcus mitis SK321

46.507

100

0.468
  radA Streptococcus mitis NCTC 12261

46.507

100

0.468

Multiple sequence alignment