Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BCEP18194_RS20780 Genome accession   NC_007510
Coordinates   3265012..3266517 (+) Length   501 a.a.
NCBI ID   WP_011353225.1    Uniprot ID   Q39CV1
Organism   Burkholderia lata     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3260012..3271517
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BCEP18194_RS20755 (Bcep18194_A6116) kdsD 3261256..3262239 (+) 984 WP_011353220.1 arabinose 5-phosphate isomerase KdsD -
  BCEP18194_RS20760 (Bcep18194_A6117) - 3262239..3262775 (+) 537 WP_011353221.1 KdsC family phosphatase -
  BCEP18194_RS20765 (Bcep18194_A6118) lptC 3262779..3263393 (+) 615 WP_011353222.1 LPS export ABC transporter periplasmic protein LptC -
  BCEP18194_RS20770 (Bcep18194_A6119) lptA 3263414..3264079 (+) 666 WP_011353223.1 lipopolysaccharide transport periplasmic protein LptA -
  BCEP18194_RS20775 (Bcep18194_A6120) lptB 3264076..3264852 (+) 777 WP_011353224.1 LPS export ABC transporter ATP-binding protein -
  BCEP18194_RS20780 (Bcep18194_A6121) rpoN/rpoN1 3265012..3266517 (+) 1506 WP_011353225.1 RNA polymerase factor sigma-54 Machinery gene
  BCEP18194_RS20785 (Bcep18194_A6122) raiA 3266676..3267035 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BCEP18194_RS20790 (Bcep18194_A6123) - 3267204..3267743 (+) 540 WP_011353226.1 PTS sugar transporter subunit IIA -
  BCEP18194_RS20795 (Bcep18194_A6124) hprK 3267873..3268841 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  BCEP18194_RS20800 (Bcep18194_A6125) rapZ 3268917..3269825 (+) 909 WP_041492961.1 RNase adapter RapZ -
  BCEP18194_RS20805 (Bcep18194_A6126) - 3269853..3270488 (+) 636 WP_011353229.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54889.02 Da        Isoelectric Point: 4.8740

>NTDB_id=24918 BCEP18194_RS20780 WP_011353225.1 3265012..3266517(+) (rpoN/rpoN1) [Burkholderia lata]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNSSSSSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKANDDALRDAHTLIRSLEPFPGAAYGKAEADYVVPDIMVKKVGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=24918 BCEP18194_RS20780 WP_011353225.1 3265012..3266517(+) (rpoN/rpoN1) [Burkholderia lata]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAACAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCAGCGGACGGATCGTTGATCGCGCAGACGCCTCCCGCGCAGAACACCGAACCCGCTGCG
ACAAACAGCAGCAGTTCGTCGAGCGATCGCGCCGAGCGCGACGAGCCGGCCGGCGCGGACGAATACGACGGTTACGGCTC
CGGCGACGGCAACGATGGCCCGCAGTGGAACCTCGACGAATTCGGGCGCTCGAGCGGCGCATCCGACGACGACGACCTCC
CGCCGCTGCAAGTGCAGGAAGCGGCGACGTCGCTGCGCGACCACCTGTCCGCGCAGTTGCGCGTCACGCAGGCGAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACGGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAGATCGATTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGGGTCGGTGCCCGCTCGGCATCCGAATGCCTGAAGCTGCAGTTGCTGCGTCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGACTTCACGCGGCTGCGCAAGCACCTGAAGGCGAA
CGACGATGCGCTGCGCGACGCGCACACGCTGATCCGTTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATGGTGAAGAAGGTCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGAGGTCGTGCCG
CGCCTGCGGATCAACAACCTCTACGCGAACATCCTGCGCAACAGCCGCGGTGATCCGTCGGCCGGTTCGCTCAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AGCGTCAAAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCTTCGTCGACGGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCTAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAAGGATTCGTGGTCGCGCGC
CGCACGGTTGCGAAGTATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q39CV1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.379

100

0.691

Multiple sequence alignment