Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CPF12_RS05925 Genome accession   NZ_CP023643
Coordinates   1258188..1259573 (+) Length   461 a.a.
NCBI ID   WP_029091568.1    Uniprot ID   A0A1D2KGY7
Organism   Brochothrix thermosphacta strain BII     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1253188..1264573
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CPF12_RS05915 (CPF12_05915) - 1254992..1255450 (+) 459 WP_029091570.1 CtsR family transcriptional regulator -
  CPF12_RS05920 (CPF12_05920) - 1255481..1257982 (+) 2502 WP_029091569.1 ATP-dependent Clp protease ATP-binding subunit -
  CPF12_RS05925 (CPF12_05925) radA 1258188..1259573 (+) 1386 WP_029091568.1 DNA repair protein RadA Machinery gene
  CPF12_RS05930 (CPF12_05930) - 1259589..1260671 (+) 1083 WP_029091567.1 PIN/TRAM domain-containing protein -
  CPF12_RS05935 (CPF12_05935) ispD 1260685..1261383 (+) 699 WP_069126479.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CPF12_RS05940 (CPF12_05940) ispF 1261386..1261862 (+) 477 WP_029091565.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CPF12_RS05945 (CPF12_05945) gltX 1261898..1263367 (+) 1470 WP_069118452.1 glutamate--tRNA ligase -
  CPF12_RS05950 (CPF12_05950) epsC 1263684..1264322 (+) 639 WP_029091563.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50638.30 Da        Isoelectric Point: 6.4051

>NTDB_id=248858 CPF12_RS05925 WP_029091568.1 1258188..1259573(+) (radA) [Brochothrix thermosphacta strain BII]
MAKKKASTKFVCNACGYESPKWMGRCPNCSEWNQMVEFVEAKVQKGRTSFNHTEEMESQQALPLSKITSETEQRILTDMP
ELNRVLGGGLVKGSLVLVGGDPGIGKSTLLLQVSAQLTTAKRKGLYVSGEESIKQTKLRAERLNVDGDLLYVYAQTDLEV
ISKTIQTLQPEFVVIDSIQTIYHPDVTSAAGSVTQVRECTSELMRLAKTLNIAIFIVGHVTKEGTIAGPRMLEHMVDTVL
YFEGERHHAYRILRAVKNRFGSTNEMGIFEMRDVGLVEVDNASEVFLEERLEGAAGSAVVTSMEGTRPVLVEIQALVSPT
VYGNARRMATGMDYNRVSLIMAVLEKREGLMLQNQDAYLKAAGGVKLDEPAIDLAIAISIASSYYDTPTAAMDCFIGEVG
LTGELRRVARIDQRVQEAAKLGFKRIIIPANNIGGWTFPAEIEVVGVSTLKDAIHKAFPNR

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=248858 CPF12_RS05925 WP_029091568.1 1258188..1259573(+) (radA) [Brochothrix thermosphacta strain BII]
ATGGCAAAGAAAAAAGCGAGTACTAAATTTGTCTGCAACGCCTGCGGATATGAGTCTCCAAAATGGATGGGGCGTTGCCC
TAACTGTAGCGAATGGAACCAAATGGTCGAATTTGTTGAGGCAAAAGTCCAAAAAGGACGAACTAGTTTTAATCATACAG
AAGAAATGGAAAGTCAGCAGGCATTGCCTCTTTCTAAAATAACAAGTGAAACTGAACAACGTATCTTAACAGATATGCCA
GAATTGAATCGTGTCCTCGGCGGGGGATTAGTTAAAGGTTCATTGGTGTTGGTCGGTGGTGACCCAGGGATTGGTAAATC
AACACTATTGTTGCAAGTATCAGCACAACTCACAACAGCAAAACGCAAAGGCCTTTATGTTTCGGGAGAAGAATCAATCA
AGCAAACAAAACTACGGGCTGAACGTTTAAATGTGGATGGGGATTTATTATATGTTTATGCGCAAACCGACTTAGAAGTC
ATCTCTAAAACAATTCAGACTTTACAGCCGGAATTTGTCGTGATTGATTCGATCCAAACCATCTATCACCCTGATGTGAC
AAGTGCAGCAGGGAGCGTCACACAAGTAAGAGAGTGTACGTCAGAATTGATGCGTCTTGCAAAGACGTTAAACATTGCCA
TCTTCATTGTCGGTCATGTGACAAAAGAGGGTACAATTGCAGGTCCACGTATGTTAGAACATATGGTCGACACTGTTTTA
TACTTTGAAGGTGAACGCCACCATGCCTACCGGATTTTACGCGCCGTAAAAAATAGGTTTGGATCTACCAATGAAATGGG
TATCTTTGAAATGCGCGATGTCGGTTTGGTAGAGGTCGACAACGCCTCAGAAGTATTTTTAGAAGAGCGTTTAGAAGGAG
CTGCTGGTTCTGCAGTTGTTACCTCAATGGAGGGGACACGTCCTGTTCTGGTAGAGATTCAAGCATTGGTGTCACCGACT
GTTTATGGAAATGCACGTCGAATGGCAACAGGAATGGATTATAATCGTGTTTCTCTAATTATGGCGGTACTTGAGAAACG
TGAAGGTTTAATGCTTCAAAATCAAGATGCCTATCTTAAAGCAGCAGGTGGTGTGAAGTTAGACGAGCCTGCAATTGATT
TAGCAATAGCAATAAGTATTGCATCGAGTTATTATGATACACCAACAGCAGCAATGGATTGCTTTATTGGTGAAGTCGGT
TTAACGGGAGAGTTACGTCGTGTTGCTCGTATTGATCAACGCGTACAAGAAGCTGCAAAACTAGGTTTCAAACGTATTAT
TATACCAGCGAACAATATCGGTGGTTGGACATTCCCTGCTGAGATTGAAGTAGTAGGTGTATCAACATTAAAAGATGCGA
TTCATAAAGCATTTCCAAATAGATAA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(366-432)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(10-37)

AAA domain (AAA_25)

(66-224)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1D2KGY7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

67.756

99.566

0.675
  radA Streptococcus pneumoniae Rx1

64.13

99.783

0.64
  radA Streptococcus pneumoniae D39

64.13

99.783

0.64
  radA Streptococcus pneumoniae R6

64.13

99.783

0.64
  radA Streptococcus pneumoniae TIGR4

64.13

99.783

0.64
  radA Streptococcus mitis SK321

64.13

99.783

0.64
  radA Streptococcus mitis NCTC 12261

64.13

99.783

0.64

Multiple sequence alignment