Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PFL01_RS24590 Genome accession   NC_007492
Coordinates   5508329..5509696 (-) Length   455 a.a.
NCBI ID   WP_007950985.1    Uniprot ID   J2XTJ9
Organism   Pseudomonas fluorescens Pf0-1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 5503329..5514696
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFL01_RS24565 (Pfl01_4880) - 5503752..5504447 (+) 696 WP_011336025.1 GntR family transcriptional regulator -
  PFL01_RS24570 (Pfl01_4881) yjiA 5504461..5505432 (-) 972 WP_011336026.1 GTPase -
  PFL01_RS24575 (Pfl01_4882) - 5505521..5505718 (-) 198 WP_003228401.1 YbdD/YjiX family protein -
  PFL01_RS24580 (Pfl01_4883) - 5505734..5507800 (-) 2067 WP_007950983.1 carbon starvation CstA family protein -
  PFL01_RS24585 (Pfl01_4884) - 5507960..5508328 (+) 369 WP_011336027.1 PilZ domain-containing protein -
  PFL01_RS24590 (Pfl01_4885) radA 5508329..5509696 (-) 1368 WP_007950985.1 DNA repair protein RadA Machinery gene
  PFL01_RS24595 (Pfl01_4886) katB 5509840..5511378 (-) 1539 WP_011336028.1 catalase KatB -
  PFL01_RS24600 (Pfl01_4887) mscL 5511664..5512077 (+) 414 WP_041475433.1 large-conductance mechanosensitive channel protein MscL -
  PFL01_RS24605 (Pfl01_4888) - 5512110..5512886 (-) 777 WP_011336030.1 ferredoxin--NADP reductase -
  PFL01_RS24610 (Pfl01_4889) - 5513192..5513899 (+) 708 WP_011336031.1 helix-turn-helix transcriptional regulator -

Sequence

Protein


Download         Length: 455 a.a.        Molecular weight: 48715.17 Da        Isoelectric Point: 6.8987

>NTDB_id=24846 PFL01_RS24590 WP_007950985.1 5508329..5509696(-) (radA) [Pseudomonas fluorescens Pf0-1]
MAKAKRMYGCTECGATFPKWAGQCGECGAWNTLTETMVESGGAAAPSGRTGWAGQQAQIKTLAEVSIEEIPRFSTASGEL
DRVLGGGLVDGSVVLIGGDPGIGKSTILLQTLCNLAKSMPALYVTGEESQQQVAMRARRLGLPQDQLRVMTETCIETIIA
TARQEKPKVMVIDSIQTIFTEQLQSAPGGVSQVRESAALLVRYAKQSGTAIFLVGHVTKEGALAGPRVLEHMVDTVLYFE
GESDGRLRLLRAVKNRFGAVNELGVFGMTDKGLKEVSNPSAIFLTRAQEEVPGSVVMATWEGTRPMLVEVQALVDDSHLA
NPRRVTLGLDQNRLAMLLAVLHRHGGIPTHDQDVFLNVVGGVKVLETASDLALMAAVMSSLRNRPLPHDLLVFGEVGLSG
EVRPVPSGQERLKEAAKHGFKRAIVPKGNAPKEAPPGLQIIAVTRLEQALDSLFE

Nucleotide


Download         Length: 1368 bp        

>NTDB_id=24846 PFL01_RS24590 WP_007950985.1 5508329..5509696(-) (radA) [Pseudomonas fluorescens Pf0-1]
ATGGCCAAGGCCAAGCGCATGTACGGCTGCACCGAGTGCGGCGCAACCTTTCCCAAGTGGGCCGGGCAGTGCGGCGAATG
CGGCGCCTGGAACACTCTGACCGAAACCATGGTCGAGAGCGGCGGTGCCGCCGCCCCGAGCGGGCGCACCGGCTGGGCCG
GGCAACAGGCCCAGATCAAGACCCTGGCCGAAGTCAGCATTGAAGAGATTCCGCGTTTTTCCACAGCCTCCGGTGAACTC
GACCGAGTCCTTGGCGGCGGTCTGGTGGACGGTTCGGTGGTGCTGATCGGCGGCGATCCCGGTATCGGCAAATCGACGAT
TCTGTTGCAGACCCTGTGCAACCTCGCCAAGAGCATGCCGGCGCTGTACGTCACCGGTGAGGAATCCCAGCAACAGGTCG
CCATGCGCGCACGCCGCCTGGGCCTGCCGCAGGATCAACTGCGGGTCATGACCGAAACCTGCATCGAAACCATCATCGCC
ACCGCCCGCCAGGAAAAACCCAAGGTGATGGTGATCGACTCGATCCAGACGATTTTCACCGAACAATTGCAATCGGCGCC
GGGCGGCGTATCCCAGGTACGCGAAAGTGCGGCGTTGCTGGTGCGTTACGCCAAGCAAAGCGGCACGGCGATTTTCCTGG
TCGGCCACGTGACCAAGGAAGGCGCGCTTGCAGGGCCGCGGGTTCTGGAACACATGGTCGATACCGTGCTGTATTTCGAA
GGCGAGTCCGATGGCCGGTTGCGTTTGCTGCGAGCGGTAAAAAACCGTTTCGGTGCCGTCAACGAACTCGGCGTGTTCGG
CATGACCGACAAGGGCCTGAAAGAAGTCTCCAACCCTTCGGCGATTTTTCTTACCCGCGCTCAGGAAGAAGTCCCGGGCA
GTGTGGTGATGGCAACGTGGGAAGGCACCCGACCGATGCTGGTGGAAGTCCAGGCACTGGTGGATGACAGCCATCTGGCC
AATCCGCGTCGGGTGACGCTGGGTCTGGACCAGAATCGTCTGGCGATGCTGTTGGCTGTTCTGCACCGGCATGGCGGCAT
TCCGACCCACGATCAGGACGTGTTCCTCAACGTGGTCGGCGGGGTGAAGGTGCTGGAAACCGCGTCTGACCTGGCGTTGA
TGGCCGCCGTCATGTCGAGCCTGCGCAACCGTCCGCTCCCCCATGATTTGCTGGTGTTTGGTGAAGTCGGCCTGTCCGGC
GAAGTGCGCCCGGTGCCGAGCGGTCAGGAGCGATTGAAGGAAGCCGCCAAGCACGGCTTCAAGCGCGCGATCGTGCCCAA
GGGCAATGCGCCGAAGGAAGCGCCTCCGGGTTTGCAGATCATTGCGGTGACGCGTCTGGAGCAGGCGCTGGACTCGTTGT
TCGAGTGA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(81-221)

Subunit ChlI of Mg-chelatase (ChlI)

(353-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB J2XTJ9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

49.234

100

0.495
  radA Streptococcus pneumoniae Rx1

46.304

100

0.468
  radA Streptococcus pneumoniae R6

46.304

100

0.468
  radA Streptococcus pneumoniae TIGR4

46.304

100

0.468
  radA Streptococcus pneumoniae D39

46.304

100

0.468
  radA Streptococcus mitis SK321

46.491

100

0.466
  radA Streptococcus mitis NCTC 12261

46.272

100

0.464

Multiple sequence alignment