Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   AOY54_RS21425 Genome accession   NZ_CP028771
Coordinates   4417082..4418578 (-) Length   498 a.a.
NCBI ID   WP_089638437.1    Uniprot ID   -
Organism   Escherichia coli strain A3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4412082..4423578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AOY54_RS21400 (AOY54_22600) panM 4412315..4412698 (+) 384 WP_000778791.1 aspartate 1-decarboxylase autocleavage activator PanM -
  AOY54_RS21405 (AOY54_22605) livJ 4412886..4413989 (-) 1104 WP_001021986.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  AOY54_RS21410 (AOY54_22615) rpoH 4414261..4415115 (-) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  AOY54_RS21415 (AOY54_22620) ftsX 4415360..4416418 (-) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  AOY54_RS21420 (AOY54_22625) ftsE 4416411..4417079 (-) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  AOY54_RS21425 (AOY54_22630) pilA 4417082..4418578 (-) 1497 WP_089638437.1 signal recognition particle-docking protein FtsY Machinery gene
  AOY54_RS21430 (AOY54_22635) rsmD 4418728..4419324 (+) 597 WP_000743189.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  AOY54_RS21435 (AOY54_22640) yhhL 4419314..4419583 (+) 270 WP_001295207.1 DUF1145 family protein -
  AOY54_RS21440 (AOY54_22645) yhhM 4419586..4419945 (-) 360 WP_000042886.1 DUF2500 domain-containing protein -
  AOY54_RS21445 (AOY54_22650) yhhN 4420086..4420712 (+) 627 WP_000964718.1 lysoplasmalogenase -
  AOY54_RS21450 (AOY54_22655) zntA 4420786..4422984 (+) 2199 WP_000106593.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  AOY54_RS21455 (AOY54_22660) tusA 4423245..4423490 (-) 246 WP_000130621.1 sulfurtransferase TusA -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54628.39 Da        Isoelectric Point: 4.1714

>NTDB_id=248273 AOY54_RS21425 WP_089638437.1 4417082..4418578(-) (pilA) [Escherichia coli strain A3]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEVAEEAVMVVSPAEEDQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=248273 AOY54_RS21425 WP_089638437.1 4417082..4418578(-) (pilA) [Escherichia coli strain A3]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGTGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGATCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACAGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCGGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCTGATTCCGCTTCTGTTATCTTCGACGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACTATTGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378

Multiple sequence alignment