Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CO697_RS14145 Genome accession   NZ_CP023529
Coordinates   2884251..2885684 (+) Length   477 a.a.
NCBI ID   WP_059177325.1    Uniprot ID   -
Organism   Lelliottia amnigena strain FDAARGOS_395     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2879251..2890684
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CO697_RS14115 (CO697_14115) - 2879819..2880796 (+) 978 WP_059177331.1 calcium/sodium antiporter -
  CO697_RS14120 (CO697_14120) kdsD 2880809..2881795 (+) 987 WP_059177330.1 arabinose-5-phosphate isomerase KdsD -
  CO697_RS14125 (CO697_14125) kdsC 2881810..2882376 (+) 567 WP_059177329.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CO697_RS14130 (CO697_14130) lptC 2882373..2882948 (+) 576 WP_059177328.1 LPS export ABC transporter periplasmic protein LptC -
  CO697_RS14135 (CO697_14135) lptA 2882917..2883471 (+) 555 WP_059177327.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  CO697_RS14140 (CO697_14140) lptB 2883478..2884203 (+) 726 WP_059177326.1 LPS export ABC transporter ATP-binding protein -
  CO697_RS14145 (CO697_14145) rpoN/rpoN1 2884251..2885684 (+) 1434 WP_059177325.1 RNA polymerase factor sigma-54 Machinery gene
  CO697_RS14150 (CO697_14150) hpf 2885707..2885994 (+) 288 WP_015960620.1 ribosome hibernation promoting factor -
  CO697_RS14155 (CO697_14155) ptsN 2886077..2886568 (+) 492 WP_059177324.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CO697_RS14160 (CO697_14160) rapZ 2886614..2887468 (+) 855 WP_015960622.1 RNase adapter RapZ -
  CO697_RS14165 (CO697_14165) npr 2887465..2887737 (+) 273 WP_015960623.1 PTS phosphocarrier protein NPr -
  CO697_RS14170 (CO697_14170) mtgA 2887792..2888517 (-) 726 WP_059177323.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  CO697_RS14175 (CO697_14175) elbB 2888514..2889167 (-) 654 WP_059177322.1 isoprenoid biosynthesis glyoxalase ElbB -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54021.90 Da        Isoelectric Point: 4.3643

>NTDB_id=248162 CO697_RS14145 WP_059177325.1 2884251..2885684(+) (rpoN/rpoN1) [Lelliottia amnigena strain FDAARGOS_395]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQEKQDTETLDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFTETDRAIATSIVDAVDDTGYLTVTLDE
ILESMGDCEIELDEIEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFAKETPRLDEARLIISEHLDLLANHDFRSLMRVTR
LKEDVLKEAVSLIQSLDPRPGQSIQTGEPEYVIPDVLVRKHNGLWTVELNSDSIPRLQINQQYASMCTSARNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQGFFEQGEEFMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTLLSDQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=248162 CO697_RS14145 WP_059177325.1 2884251..2885684(+) (rpoN/rpoN1) [Lelliottia amnigena strain FDAARGOS_395]
ATGAAGCAAGGTTTGCAATTACGGCTCAGCCAACAGTTGGCGATGACGCCACAGTTACAACAGGCAATCCGTCTGTTGCA
ACTGTCAACGTTAGAACTTCAGCAGGAACTGCAGCAAGCGCTGGACAGTAATCCACTGCTGGAGCAAACCGATCTTCATG
ATGAGGTAGATACCCAGGAAAAGCAGGACACCGAAACGCTCGATACGGCAGATGCCCTTGAGCAGAAAGAGATGCCTGAC
GAGTTACCGCTGGATGCCAGCTGGGATGAAATTTACACCGCTGGAACCCCATCCGGCACGCGCGCTGATTATCAGGATGA
TGAGCTACCGGTTTATCAGGGTGAAACTACGCAGTCGCTACAGGATTATCTGATGTGGCAGGTGGAGTTGACCCCTTTCA
CCGAAACCGATCGTGCGATTGCCACGTCCATTGTCGATGCCGTGGATGACACCGGGTATTTAACGGTAACCCTGGACGAG
ATTCTGGAAAGCATGGGTGATTGCGAAATCGAACTCGACGAGATCGAAGCCGTTCTCAAGCGCATTCAACGCTTTGACCC
TGTCGGCGTCGCAGCAAAAGATCTGCGCGACTGCTTGCTGATCCAGCTTTCTCAGTTTGCAAAAGAGACGCCAAGACTCG
ACGAAGCGCGGCTGATTATCAGCGAACATTTGGATTTGCTGGCGAATCATGATTTCCGCAGTCTGATGCGCGTTACGCGA
CTCAAAGAAGACGTGCTGAAAGAAGCGGTGAGTCTGATCCAGTCGCTCGATCCCCGTCCGGGCCAATCGATTCAAACCGG
CGAACCCGAATATGTGATTCCTGACGTTCTGGTCCGAAAGCACAACGGTCTCTGGACCGTAGAACTCAATTCCGACAGCA
TTCCCCGCCTGCAAATCAACCAGCAATACGCGTCTATGTGCACCAGTGCGCGGAACGACTCCGACAATCAATATATTCGT
AGCAATCTTCAGGAAGCGCGATGGCTGATCAAAAGCCTCGAAAGCCGAAATGATACGCTGCTACGCGTGAGTCGATGCAT
TGTGGAACAGCAGCAAGGCTTTTTTGAGCAGGGCGAAGAATTTATGAAACCGATGGTCCTGGCAGATATTGCCCAGGCCG
TTGAGATGCACGAATCGACCATTTCGCGTGTGACCACGCAGAAGTATCTGCACAGTCCGCGTGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGTCATGTGAATACCGAAGGCGGTGGCGAAGCCTCGTCGACGGCCATTCGTGCTCTGGTGAAGAAGTT
GATCGCCGCGGAAAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCCTGCTGTCCGATCAGGGTATCATGGTGG
CACGTCGTACTGTTGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCTAACCAGCGTAAACAACTGGTTTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.347

100

0.48

Multiple sequence alignment