Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   BURPS1710B_RS03605 Genome accession   NC_007434
Coordinates   776377..777885 (-) Length   502 a.a.
NCBI ID   WP_004526062.1    Uniprot ID   A0A0E1W9G0
Organism   Burkholderia pseudomallei 1710b     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 771377..782885
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BURPS1710B_RS03580 (BURPS1710b_0760) - 772391..773023 (-) 633 WP_004195230.1 LON peptidase substrate-binding domain-containing protein -
  BURPS1710B_RS03585 (BURPS1710b_0761) rapZ 773050..773943 (-) 894 WP_004195227.1 RNase adapter RapZ -
  BURPS1710B_RS03590 (BURPS1710b_0762) hprK 774029..774997 (-) 969 WP_004195225.1 HPr(Ser) kinase/phosphatase -
  BURPS1710B_RS03595 (BURPS1710b_0763) ptsN 775126..775635 (-) 510 WP_004534308.1 PTS IIA-like nitrogen regulatory protein PtsN -
  BURPS1710B_RS03600 (BURPS1710b_0764) hpf 775868..776227 (-) 360 WP_004195219.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  BURPS1710B_RS03605 (BURPS1710b_0765) rpoN/rpoN1 776377..777885 (-) 1509 WP_004526062.1 RNA polymerase factor sigma-54 Machinery gene
  BURPS1710B_RS03610 (BURPS1710b_0766) lptB 778054..778830 (-) 777 WP_004195214.1 LPS export ABC transporter ATP-binding protein -
  BURPS1710B_RS03615 (BURPS1710b_0767) lptA 778827..779492 (-) 666 WP_004195213.1 lipopolysaccharide transport periplasmic protein LptA -
  BURPS1710B_RS03620 (BURPS1710b_0768) lptC 779511..780125 (-) 615 WP_004522855.1 LPS export ABC transporter periplasmic protein LptC -
  BURPS1710B_RS03625 (BURPS1710b_0769) - 780131..780670 (-) 540 WP_004200146.1 KdsC family phosphatase -
  BURPS1710B_RS03630 (BURPS1710b_0770) kdsD 780670..781653 (-) 984 WP_004195206.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 502 a.a.        Molecular weight: 54875.02 Da        Isoelectric Point: 4.8740

>NTDB_id=24772 BURPS1710B_RS03605 WP_004526062.1 776377..777885(-) (rpoN/rpoN1) [Burkholderia pseudomallei 1710b]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVATAIAQNPLLENDDDWIASPLRVAADGSVIAQTPASSAPEPMA
GAPNGTAATNERTERDETAGVDEYDSYSADGGDASQWNLDDFGRSPAASDDDDLPPMQIHETSTSLRDHLMAQLRVTQAS
PRDRALVTFLIESLDDDGYLSASLEEVLADLPAELEVDVDELNAGLALVHSFDPAGVGARSASECLKLQLLRLDPSPTRA
LALDIVSQHLELLAARDFTRLRKQLKATDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQNWQAELNPEVV
PKLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTL
GLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVA
RRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1509 bp        

>NTDB_id=24772 BURPS1710B_RS03605 WP_004526062.1 776377..777885(-) (rpoN/rpoN1) [Burkholderia pseudomallei 1710b]
ATGAAAGCCAGTCTTCAACTTCGCCTGTCGCAGCATCTCGCGCTCACGCCCCAGCTTCAGCAGTCGATCCGGCTGCTGCA
ACTGTCGACGCTCGAACTGCAACAGGAAGTCGCGACGGCGATCGCGCAGAACCCGCTGCTCGAGAACGACGATGACTGGA
TCGCGAGCCCGTTGCGCGTCGCGGCCGACGGCTCGGTGATCGCGCAGACGCCCGCGTCGTCCGCGCCCGAGCCGATGGCG
GGCGCGCCGAACGGCACGGCTGCGACCAATGAGCGCACCGAGCGCGACGAAACCGCCGGCGTCGACGAATACGACAGCTA
TTCGGCCGACGGCGGCGACGCGAGCCAGTGGAACCTCGACGACTTCGGCCGCTCGCCCGCCGCGTCCGACGACGACGATC
TGCCGCCGATGCAGATCCACGAGACGTCGACGTCGCTGCGCGATCATCTGATGGCGCAGTTGCGCGTCACGCAGGCGAGC
CCGCGCGATCGCGCGCTCGTCACGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGAGCGCGTCGCTCGAGGAAGT
GCTCGCCGATCTGCCGGCGGAGCTCGAGGTCGACGTCGACGAACTGAACGCGGGGCTCGCGCTCGTGCACAGCTTCGATC
CGGCGGGCGTCGGCGCGCGCTCCGCGTCCGAATGCCTGAAGCTGCAGTTGCTGCGCCTGGATCCGTCCCCGACGCGCGCG
CTCGCGCTCGATATCGTGTCGCAGCATCTGGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTGCGCAAGCAACTGAAGGC
GACCGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTCGAGCCGTTCCCCGGCGCGGCGTACGGCAAGGCCG
AAGCCGATTACGTGGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGAACTGGCAGGCGGAACTCAATCCGGAAGTGGTG
CCGAAGCTGCGGATCAACAATCTTTACGCGAACATCCTGCGCAACAGCCGGGGCGATCCGAGCGCGGGATCGCTGAAGCA
ACAGCTTCAGGAAGCGCGCTGGCTGATCAAGAACATTCAGCAGCGGTTCGAGACGATCCTGCGCGTCGCGCAGGCGATTG
TCGAGCGTCAGAAGAATTTCTTCGCGCACGGCGAAATTGCGATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTG
GGCCTACACGAGTCGACTGTCTCCCGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGCACACTTGAATTTAAGTA
CTTCTTCGGATCGCACGTATCGACAGACACCGGAGGTGCCGCGTCGTCGACAGCGATCCGCGCCCTCATCAAACAACTGA
TAGGAGCCGAAGACCCCAAATCCCCTCTTTCGGACAGTCGCATAGCCGAGCTGCTAGCGGAACAAGGGTTCGTGGTCGCG
CGCCGCACGGTTGCCAAATATCGCGAAGCCCTCAAGATCCCGGCGGTGAATCTGCGCAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(138-329)

Sigma-54, DNA binding domain (Sigma54_DBD)

(345-500)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E1W9G0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.515

100

0.689

Multiple sequence alignment