Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC71_RS14870 Genome accession   NZ_CP023485
Coordinates   2942564..2943580 (-) Length   338 a.a.
NCBI ID   WP_020839764.1    Uniprot ID   -
Organism   Vibrio parahaemolyticus strain FORC_071     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2937564..2948580
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC71_RS14845 (FORC71_2654) aroK 2938424..2938942 (-) 519 WP_005381319.1 shikimate kinase AroK -
  FORC71_RS14850 (FORC71_2655) pilQ 2939131..2940879 (-) 1749 WP_005488227.1 type IV pilus secretin PilQ family protein Machinery gene
  FORC71_RS14855 (FORC71_2656) pilP 2940911..2941426 (-) 516 WP_005480477.1 pilus assembly protein PilP Machinery gene
  FORC71_RS14860 (FORC71_2657) pilO 2941416..2942009 (-) 594 WP_025631084.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC71_RS14865 (FORC71_2658) pilN 2942002..2942580 (-) 579 WP_005480484.1 PilN domain-containing protein Machinery gene
  FORC71_RS14870 (FORC71_2659) pilM 2942564..2943580 (-) 1017 WP_020839764.1 type IV pilus assembly protein PilM Machinery gene
  FORC71_RS14875 (FORC71_2660) - 2943723..2946293 (+) 2571 WP_005496567.1 PBP1A family penicillin-binding protein -
  FORC71_RS14880 (FORC71_2661) oxyR 2946350..2947258 (-) 909 WP_005465160.1 DNA-binding transcriptional regulator OxyR -
  FORC71_RS14885 (FORC71_2662) - 2947429..2948157 (+) 729 WP_015297397.1 redoxin family protein -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 37762.28 Da        Isoelectric Point: 6.8322

>NTDB_id=247638 FORC71_RS14870 WP_020839764.1 2942564..2943580(-) (pilM) [Vibrio parahaemolyticus strain FORC_071]
MDKLIVTGIDIGHNSLKAVVLKPIGDQYALLGYKEILLKEGIVAENNTINHQEIVKTLKQMKKELPFGAKRVAISVPDNS
VISKKLQIEQSLDESEIEFAVVQAFSHQSPFPVEELSLDFVRLRAEEGMRGTDSYQVFATRKDVVESRVEALQQSGLKPV
LVDVHSQSLGHIWKLAAERFPDKNKYCLLDIGSLASSFTMFTEQGELFHKEFACGTRISMGTSQEDLLSDDAQAKTEQFN
RQVVERVKRQMQLYTSINGSQNIKGIWLSGEGASTPMLAEELSHQLALECELLNPLGLFEMKVSKRKRRAADWQHFSTAA
GLAVRGIHWLGGVRALSH

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=247638 FORC71_RS14870 WP_020839764.1 2942564..2943580(-) (pilM) [Vibrio parahaemolyticus strain FORC_071]
ATGGATAAGCTAATCGTTACCGGCATAGATATTGGCCATAACAGCCTCAAAGCCGTAGTACTTAAACCCATTGGCGATCA
ATACGCCTTATTGGGTTACAAAGAAATATTACTTAAGGAAGGTATTGTCGCTGAAAACAACACTATAAATCATCAAGAAA
TTGTAAAGACCCTAAAGCAGATGAAAAAAGAGCTGCCTTTTGGGGCAAAGCGAGTGGCAATTTCTGTTCCAGACAACTCA
GTTATTAGCAAGAAACTGCAAATCGAACAAAGCCTTGATGAGAGCGAAATCGAGTTTGCGGTGGTACAAGCTTTTTCTCA
TCAATCTCCTTTTCCTGTGGAAGAACTGAGTTTGGATTTCGTACGCCTTCGTGCGGAAGAAGGGATGCGTGGAACGGACA
GTTATCAAGTATTCGCTACGCGTAAAGATGTGGTTGAAAGCCGAGTTGAGGCGTTACAACAGTCTGGTTTAAAGCCTGTG
TTGGTTGACGTGCACTCTCAAAGCCTAGGTCATATCTGGAAGCTTGCCGCAGAGCGTTTTCCTGATAAAAACAAATACTG
TTTGTTAGACATCGGCTCGTTAGCGAGTTCATTCACCATGTTTACCGAGCAAGGCGAACTGTTTCATAAGGAGTTTGCGT
GCGGTACTCGTATTTCAATGGGGACGTCACAAGAAGACTTACTCTCTGATGATGCTCAAGCCAAAACGGAACAGTTCAAT
CGTCAAGTGGTTGAGCGCGTAAAACGTCAAATGCAGCTCTATACCTCGATCAATGGTTCGCAAAACATCAAAGGCATTTG
GTTATCGGGCGAGGGGGCATCGACGCCAATGTTGGCAGAAGAGCTCTCGCATCAGTTGGCATTGGAGTGCGAGTTGCTAA
ACCCACTCGGACTGTTTGAAATGAAAGTGTCGAAACGCAAGCGACGAGCGGCTGATTGGCAACATTTTTCGACAGCTGCA
GGGCTGGCGGTCCGTGGCATTCATTGGTTAGGAGGTGTGCGTGCTTTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

84.615

100

0.846
  pilM Vibrio cholerae strain A1552

49.263

100

0.494

Multiple sequence alignment