Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   CLI98_RS00395 Genome accession   NZ_CP023431
Coordinates   60093..60701 (+) Length   202 a.a.
NCBI ID   WP_014418235.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB 574     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 55093..65701
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI98_RS00355 (CLI98_00067) - 56113..56619 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  CLI98_RS00360 (CLI98_00068) - 56758..57171 (+) 414 WP_017418017.1 hypothetical protein -
  CLI98_RS00365 (CLI98_00069) - 57201..57668 (-) 468 WP_017418018.1 YppG family protein -
  CLI98_RS00370 (CLI98_00070) - 57855..58037 (+) 183 WP_003153532.1 YppF family protein -
  CLI98_RS00375 (CLI98_00071) - 58076..58444 (-) 369 WP_014418232.1 YppE family protein -
  CLI98_RS00380 (CLI98_00072) - 58490..58735 (-) 246 WP_014418233.1 DUF5446 family protein -
  CLI98_RS00385 (CLI98_00073) sspM 58941..59045 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  CLI98_RS00390 (CLI98_00074) - 59089..60051 (-) 963 WP_022553046.1 DUF2515 domain-containing protein -
  CLI98_RS00395 (CLI98_00075) recU 60093..60701 (+) 609 WP_014418235.1 Holliday junction resolvase RecU Machinery gene
  CLI98_RS00400 (CLI98_00076) - 60740..63523 (+) 2784 WP_022553045.1 PBP1A family penicillin-binding protein -
  CLI98_RS00405 (CLI98_00077) - 63601..64092 (-) 492 WP_022553044.1 YpoC family protein -
  CLI98_RS00410 (CLI98_00078) nth 64089..64748 (-) 660 WP_022553043.1 endonuclease III -
  CLI98_RS00415 (CLI98_00079) dnaD 64767..65465 (-) 699 WP_003153516.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23680.82 Da        Isoelectric Point: 9.6872

>NTDB_id=247035 CLI98_RS00395 WP_014418235.1 60093..60701(+) (recU) [Bacillus velezensis strain SCGB 574]
MIRYPNGKTFQPNKTVSSQNSQKRTHSYSNRGMTLEDDLNETNKYYLANQIAVIHKKPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWDRKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=247035 CLI98_RS00395 WP_014418235.1 60093..60701(+) (recU) [Bacillus velezensis strain SCGB 574]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGACCCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAAGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTTGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGACAGAAAGGAG
AAGAACGGCAGAAAATCGATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

91.457

98.515

0.901

Multiple sequence alignment