Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   CMR26_RS16900 Genome accession   NZ_CP023414
Coordinates   3399192..3400304 (-) Length   370 a.a.
NCBI ID   WP_007409910.1    Uniprot ID   -
Organism   Bacillus velezensis strain BS-37     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 3374022..3418154 3399192..3400304 within 0


Gene organization within MGE regions


Location: 3374022..3418154
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMR26_RS16785 (CMR26_16805) dnaX 3374022..3375713 (-) 1692 WP_007408753.1 DNA polymerase III subunit gamma/tau -
  CMR26_RS16795 (CMR26_16815) tadA 3376196..3376678 (-) 483 WP_108725197.1 tRNA adenosine(34) deaminase TadA -
  CMR26_RS16800 (CMR26_16820) - 3376800..3377312 (+) 513 WP_042635751.1 cysteine hydrolase family protein -
  CMR26_RS16805 (CMR26_16825) - 3377384..3378667 (+) 1284 WP_032868303.1 glycoside hydrolase family 18 protein -
  CMR26_RS16810 (CMR26_16830) dgk 3378763..3379386 (+) 624 WP_032870958.1 deoxyguanosine kinase -
  CMR26_RS16815 (CMR26_16835) dck 3379383..3380036 (+) 654 WP_007615132.1 deoxyadenosine/deoxycytidine kinase -
  CMR26_RS16825 (CMR26_16845) - 3380476..3381621 (+) 1146 WP_061046466.1 glycerate kinase -
  CMR26_RS16830 (CMR26_16850) serS 3381632..3382909 (-) 1278 WP_007408744.1 serine--tRNA ligase -
  CMR26_RS16835 (CMR26_16855) pdxT 3383229..3383819 (-) 591 WP_007615126.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  CMR26_RS16840 (CMR26_16860) pdxS 3383841..3384725 (-) 885 WP_003150714.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  CMR26_RS16845 (CMR26_16865) dacA 3384922..3386253 (-) 1332 WP_020955205.1 D-alanyl-D-alanine carboxypeptidase -
  CMR26_RS16850 (CMR26_16870) guaB 3386406..3387872 (-) 1467 WP_007408741.1 IMP dehydrogenase -
  CMR26_RS16855 (CMR26_16875) - 3387993..3388943 (+) 951 WP_039062454.1 YaaC family protein -
  CMR26_RS16885 (CMR26_16905) gyrA 3394281..3396740 (-) 2460 WP_015239009.1 DNA gyrase subunit A -
  CMR26_RS16890 (CMR26_16910) gyrB 3396956..3398872 (-) 1917 WP_011996153.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  CMR26_RS16895 (CMR26_16915) remB 3398929..3399174 (-) 246 WP_004392908.1 extracellular matrix regulator RemB -
  CMR26_RS16900 (CMR26_16920) recF 3399192..3400304 (-) 1113 WP_007409910.1 DNA replication/repair protein RecF Machinery gene
  CMR26_RS16905 (CMR26_16925) rlbA 3400320..3400535 (-) 216 WP_004392910.1 ribosome maturation protein RlbA -
  CMR26_RS16910 (CMR26_16930) dnaN 3400670..3401806 (-) 1137 WP_014416704.1 DNA polymerase III subunit beta -
  CMR26_RS16915 (CMR26_16935) dnaA 3401991..3403331 (-) 1341 WP_007409908.1 chromosomal replication initiator protein DnaA -
  CMR26_RS16920 (CMR26_16940) rpmH 3403953..3404087 (+) 135 WP_003178075.1 50S ribosomal protein L34 -
  CMR26_RS16925 (CMR26_16945) rnpA 3404241..3404591 (+) 351 WP_004392944.1 ribonuclease P protein component -
  CMR26_RS16930 (CMR26_16950) spoIIIJ 3404752..3405501 (+) 750 WP_007409906.1 YidC family membrane integrase SpoIIIJ -
  CMR26_RS16935 (CMR26_16955) jag 3405498..3406139 (+) 642 WP_032868209.1 RNA-binding cell elongation regulator Jag/EloR -
  CMR26_RS16940 (CMR26_16960) mnmE 3406429..3407808 (+) 1380 WP_108725198.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  CMR26_RS16945 (CMR26_16965) mnmG 3407825..3409711 (+) 1887 WP_007615086.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  CMR26_RS16950 (CMR26_16970) rsmG 3409725..3410444 (+) 720 WP_007409901.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  CMR26_RS16955 (CMR26_16975) noc 3410566..3411417 (+) 852 WP_007409900.1 nucleoid occlusion protein -
  CMR26_RS16960 (CMR26_16980) soj 3411716..3412477 (+) 762 WP_007409899.1 sporulation initiation inhibitor protein Soj -
  CMR26_RS16965 (CMR26_16985) - 3412470..3413321 (+) 852 WP_004392966.1 ParB/RepB/Spo0J family partition protein -
  CMR26_RS16970 (CMR26_16990) yyaC 3413351..3413965 (-) 615 WP_004392969.1 spore protease YyaC -
  CMR26_RS16975 (CMR26_16995) - 3414140..3414643 (-) 504 WP_020958120.1 GNAT family N-acetyltransferase -
  CMR26_RS16980 (CMR26_17000) - 3414787..3415812 (+) 1026 WP_088005468.1 hypothetical protein -
  CMR26_RS16985 (CMR26_17005) - 3415898..3416104 (+) 207 WP_004392973.1 DUF951 domain-containing protein -
  CMR26_RS16990 (CMR26_17010) - 3416151..3418154 (+) 2004 WP_032868214.1 molybdopterin-dependent oxidoreductase -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42430.36 Da        Isoelectric Point: 7.0882

>NTDB_id=246913 CMR26_RS16900 WP_007409910.1 3399192..3400304(-) (recF) [Bacillus velezensis strain BS-37]
MYIQNLELTSYRNYERAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDEDYAKIEGRVMKRNGD
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSAVYLYDLSLYQKILSQR
NHFLKQLQSRKQTDRTMLDVLTDQLIEAAAKVVAKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALDVSDPKDLSKI
GNSYQESFSKLKEKEIERGVTLFGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHDTLHQAGMFRVENGTLVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=246913 CMR26_RS16900 WP_007409910.1 3399192..3400304(-) (recF) [Bacillus velezensis strain BS-37]
TTGTATATCCAAAATTTAGAATTAACATCCTACCGTAATTATGAGCGCGCTGAGCTTCAATTCGAAAACAAGGTGAATGT
GATCATCGGGGAAAATGCCCAAGGCAAAACCAATCTGATGGAAGCCATCTATGTCCTTTCGATGGCAAAATCACACCGCA
CCTCAAATGACAAAGAACTTATACGGTGGGACGAAGACTATGCTAAAATAGAAGGAAGAGTGATGAAGCGAAACGGGGAT
ATCCCGATGCAGCTTGTCATCTCCAAAAAAGGTAAAAAGGGCAAGGTCAATCATATAGAGCAGCAAAAACTCAGTCAGTA
TGTCGGAGCGCTGAACACCATTATGTTTGCCCCTGAGGATTTAAATCTCGTCAAAGGAAGCCCCCAGGTGAGGAGGAGAT
TTCTCGATATGGAGATCGGGCAGGTTTCTGCCGTTTATCTGTATGATTTATCTCTTTATCAAAAAATCCTTTCACAGCGG
AATCATTTTCTGAAACAGCTGCAAAGCCGGAAACAGACTGACCGGACCATGCTTGACGTTCTCACTGATCAGCTGATTGA
AGCAGCCGCAAAAGTTGTCGCCAAACGTCTGCAGTTTACGGCGCAGCTTGAAAAATGGGCGCAGCCGATCCATTCCGGCA
TTTCCCGGGGACTTGAAGAACTGACGCTTAAGTACCACACGGCGCTTGATGTATCAGATCCCAAAGATTTGTCGAAAATA
GGTAACAGCTACCAGGAGTCTTTTTCGAAATTAAAAGAAAAAGAAATAGAACGAGGCGTCACATTATTCGGACCGCATCG
GGATGATGTCCTTTTTTATGTGAACGGACGCGATGTGCAGACGTACGGTTCGCAGGGACAGCAGCGTACGACGGCTTTGT
CTTTGAAGCTGGCCGAAATAGATTTGATTCATGAAGAAATCGGAGAATACCCCATTTTACTTTTGGATGATGTACTAAGC
GAGCTGGATGATTACCGACAGTCCCATTTGCTTCATACGATCCAAGGCCGTGTGCAGACATTTGTCACCACGACAAGCGT
GGATGGCATTGACCACGATACCTTACATCAAGCAGGAATGTTCCGTGTGGAAAACGGTACGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-348)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

94.324

100

0.943

Multiple sequence alignment