Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CMR26_RS15120 Genome accession   NZ_CP023414
Coordinates   3068854..3069291 (+) Length   145 a.a.
NCBI ID   WP_032867344.1    Uniprot ID   -
Organism   Bacillus velezensis strain BS-37     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3063854..3074291
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMR26_RS15105 (CMR26_15125) - 3066713..3067075 (-) 363 WP_007409352.1 winged helix-turn-helix transcriptional regulator -
  CMR26_RS15110 (CMR26_15130) hxlA 3067307..3067942 (+) 636 WP_015239130.1 3-hexulose-6-phosphate synthase -
  CMR26_RS15115 (CMR26_15135) hxlB 3067939..3068496 (+) 558 WP_015239129.1 6-phospho-3-hexuloisomerase -
  CMR26_RS15120 (CMR26_15140) nucA/comI 3068854..3069291 (+) 438 WP_032867344.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CMR26_RS15125 (CMR26_15145) nin/comJ 3069312..3069710 (+) 399 WP_015239128.1 competence protein ComJ Regulator
  CMR26_RS15130 (CMR26_15150) - 3069751..3071187 (-) 1437 WP_007409356.1 glycoside hydrolase family 1 protein -
  CMR26_RS15135 (CMR26_15155) - 3071291..3071569 (-) 279 WP_263612330.1 YckD family protein -
  CMR26_RS15140 (CMR26_15160) - 3071686..3072120 (-) 435 WP_007409358.1 RDD family protein -
  CMR26_RS15145 (CMR26_15165) - 3072135..3073271 (-) 1137 WP_007409359.1 zinc-dependent alcohol dehydrogenase -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16094.41 Da        Isoelectric Point: 9.0073

>NTDB_id=246906 CMR26_RS15120 WP_032867344.1 3068854..3069291(+) (nucA/comI) [Bacillus velezensis strain BS-37]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSHYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=246906 CMR26_RS15120 WP_032867344.1 3068854..3069291(+) (nucA/comI) [Bacillus velezensis strain BS-37]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTCATTACGACAAAGTAATCTTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGACATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCCGTCAAAACGGGATACGATAGAGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAATAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment