Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CMR26_RS08080 Genome accession   NZ_CP023414
Coordinates   1601967..1602401 (-) Length   144 a.a.
NCBI ID   WP_007611576.1    Uniprot ID   A0A172XIP3
Organism   Bacillus velezensis strain BS-37     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 1596967..1607401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMR26_RS08045 (CMR26_08050) - 1596981..1597334 (+) 354 WP_007410379.1 DMT family transporter -
  CMR26_RS08050 (CMR26_08055) - 1597348..1597800 (-) 453 WP_007410380.1 OsmC family protein -
  CMR26_RS08055 (CMR26_08060) - 1597860..1598567 (-) 708 WP_007410381.1 poly-gamma-glutamate hydrolase family protein -
  CMR26_RS08060 (CMR26_08065) - 1598823..1599056 (-) 234 WP_015239897.1 hypothetical protein -
  CMR26_RS08065 (CMR26_08070) - 1599235..1600563 (+) 1329 WP_047935736.1 S8 family peptidase -
  CMR26_RS08070 (CMR26_08075) - 1600756..1601118 (+) 363 WP_007410383.1 hypothetical protein -
  CMR26_RS08075 (CMR26_08080) - 1601152..1601907 (-) 756 WP_003154084.1 YoaK family protein -
  CMR26_RS08080 (CMR26_08085) nucA/comI 1601967..1602401 (-) 435 WP_007611576.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CMR26_RS08085 (CMR26_08090) - 1602690..1603901 (+) 1212 WP_007409852.1 cytochrome P450 family protein -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 15528.47 Da        Isoelectric Point: 7.2418

>NTDB_id=246878 CMR26_RS08080 WP_007611576.1 1601967..1602401(-) (nucA/comI) [Bacillus velezensis strain BS-37]
MNAFMKWAASLLLVISLQFGLTGAGIHSDSAARAASRYDQVLYFPLSKYPETGNHIKDAISAGHSEICTIDRGGAENRRK
ESLKGIPTKPGFDRDEWPMAVCTEGGAGADIRYVTPSDNRGAGSWVGNQMSGYSDGTRVLFIVQ

Nucleotide


Download         Length: 435 bp        

>NTDB_id=246878 CMR26_RS08080 WP_007611576.1 1601967..1602401(-) (nucA/comI) [Bacillus velezensis strain BS-37]
ATGAATGCGTTTATGAAATGGGCGGCAAGTCTGCTTTTGGTGATCTCTCTTCAGTTCGGCCTTACGGGCGCCGGCATCCA
TTCGGACAGTGCTGCTCGTGCCGCATCCCGATACGATCAGGTGCTGTATTTCCCGCTGTCAAAATATCCGGAGACGGGAA
ATCATATAAAAGACGCCATTTCGGCAGGTCATTCTGAGATTTGTACAATTGATCGGGGCGGAGCGGAGAATAGAAGGAAA
GAATCATTGAAAGGAATTCCGACAAAGCCCGGGTTTGACCGTGATGAATGGCCCATGGCAGTCTGCACAGAAGGCGGGGC
GGGGGCTGATATCAGATATGTAACCCCGTCGGATAACCGCGGAGCCGGTTCATGGGTTGGAAATCAAATGAGCGGATATT
CCGACGGCACGAGAGTATTATTTATCGTTCAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-138)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A172XIP3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

56.452

86.111

0.486

Multiple sequence alignment