Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   CMV25_RS03350 Genome accession   NZ_CP023392
Coordinates   690673..692034 (-) Length   453 a.a.
NCBI ID   WP_096039573.1    Uniprot ID   A0A290PX25
Organism   Lactococcus raffinolactis strain WiKim0068     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 685673..697034
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CMV25_RS03325 (CMV25_03325) gltX 685815..687263 (-) 1449 WP_096039569.1 glutamate--tRNA ligase -
  CMV25_RS03330 (CMV25_03330) ispF 687408..687890 (-) 483 WP_096039570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  CMV25_RS03335 (CMV25_03335) - 687877..688638 (-) 762 WP_061774662.1 TIGR00266 family protein -
  CMV25_RS03340 (CMV25_03340) ispD 688694..689374 (-) 681 WP_096039571.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  CMV25_RS03345 (CMV25_03345) - 689371..690444 (-) 1074 WP_096039572.1 PIN/TRAM domain-containing protein -
  CMV25_RS03350 (CMV25_03350) radA 690673..692034 (-) 1362 WP_096039573.1 DNA repair protein RadA Machinery gene
  CMV25_RS03355 (CMV25_03355) - 692036..692482 (-) 447 WP_096039574.1 dUTP diphosphatase -
  CMV25_RS03360 (CMV25_03360) - 692559..694316 (-) 1758 WP_096039575.1 ABC transporter ATP-binding protein -
  CMV25_RS03365 (CMV25_03365) - 694309..696027 (-) 1719 WP_096039576.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49326.82 Da        Isoelectric Point: 5.4218

>NTDB_id=246591 CMV25_RS03350 WP_096039573.1 690673..692034(-) (radA) [Lactococcus raffinolactis strain WiKim0068]
MAKAKSKFVCQSCGYQSPKYLGRCPNCGAWNSFVEEIEAKEVKNARVTLTGEKTRPVKLGEVESLDTPRVLTDMGEFNRV
LGGGVVPGSLILIGGDPGIGKSTILLQVSVQLANLGRVLYVSGEESAQQIKMRAERLGDLDSDFYLYAENNMQNIRAEIE
KLQPDFLVIDSIQTVMSPEITGVQGSVSQVREVTAELLQIAKTNNIATFIVGHVTKEGSLAGPRMLEHMVDTVLYFEGER
QHTFRILRAVKNRFGSTNEIGIFEMQSQGLVEVTNPSEIFLEERLEGATGSAIVVSMEGTRPILVEIQALVTPTIFGNAR
RTTSGLDFNRVSLIMAVLEKRANLLLQNQDAYLKSAGGVKLDEPAIDLAIAVAIASSYKEKPTAPDVAFIGEIGLTGEIR
RVNRIESRINEAAKLGFKKLYAPKNSLAGLEIPPSIEVIGVTTLAEVLQKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=246591 CMV25_RS03350 WP_096039573.1 690673..692034(-) (radA) [Lactococcus raffinolactis strain WiKim0068]
ATGGCGAAAGCAAAATCAAAATTCGTCTGCCAATCCTGTGGGTATCAGTCGCCCAAATATCTGGGGCGCTGTCCTAATTG
TGGCGCTTGGAACTCTTTTGTCGAAGAAATTGAAGCCAAAGAGGTCAAAAATGCGCGCGTGACCCTGACTGGGGAGAAAA
CACGACCTGTTAAACTAGGAGAAGTCGAAAGTCTAGATACACCCCGTGTTTTGACAGATATGGGCGAGTTTAACCGCGTT
CTTGGCGGTGGCGTTGTACCGGGGAGCTTGATTCTTATCGGTGGTGATCCAGGGATTGGGAAGTCAACCATTCTCTTACA
AGTTTCTGTGCAGCTGGCAAATCTCGGGCGTGTCCTTTATGTGTCAGGCGAGGAATCAGCCCAACAAATCAAGATGCGAG
CTGAACGTTTGGGTGATTTGGATTCTGATTTCTACCTCTACGCAGAAAATAATATGCAAAATATCCGAGCTGAAATTGAA
AAATTGCAGCCAGATTTTCTGGTCATTGACTCCATCCAAACCGTCATGAGCCCCGAAATTACAGGCGTACAAGGCTCGGT
CAGTCAAGTTCGAGAAGTGACCGCAGAACTACTCCAAATCGCAAAAACCAATAATATTGCTACCTTTATCGTCGGTCACG
TCACCAAGGAAGGCAGTCTTGCCGGCCCGAGAATGCTAGAGCATATGGTAGATACGGTGCTTTATTTTGAAGGGGAGCGC
CAGCATACTTTTCGGATTCTTCGTGCGGTGAAAAACCGGTTCGGCTCAACCAATGAAATCGGAATTTTTGAGATGCAATC
TCAAGGACTGGTCGAAGTGACCAATCCTAGCGAGATTTTCTTAGAAGAACGTTTAGAAGGCGCGACTGGCTCTGCTATTG
TTGTTTCAATGGAAGGGACCAGACCGATTTTGGTAGAAATTCAAGCGCTTGTCACACCAACGATTTTTGGTAATGCCAGA
CGAACAACTTCAGGTCTTGACTTTAATCGGGTCAGCTTGATCATGGCAGTGCTTGAAAAACGTGCCAATCTCCTGCTCCA
AAATCAAGATGCCTATCTCAAATCAGCTGGTGGTGTCAAATTAGATGAACCAGCCATTGATTTAGCCATTGCGGTTGCCA
TTGCTTCAAGTTATAAAGAAAAACCAACAGCACCAGATGTTGCCTTTATCGGTGAAATTGGCTTGACGGGCGAAATTCGC
CGGGTCAATCGCATCGAGAGTCGGATCAATGAGGCAGCTAAACTAGGCTTTAAAAAACTTTATGCCCCTAAAAATTCCTT
GGCTGGTTTAGAAATCCCACCATCTATAGAAGTCATTGGTGTCACGACTTTAGCCGAAGTCTTACAGAAGGTCTTTGCTT
AA

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(82-218)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A290PX25

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

81.678

100

0.817
  radA Streptococcus pneumoniae D39

81.678

100

0.817
  radA Streptococcus pneumoniae R6

81.678

100

0.817
  radA Streptococcus pneumoniae TIGR4

81.678

100

0.817
  radA Streptococcus mitis SK321

81.678

100

0.817
  radA Streptococcus mitis NCTC 12261

81.457

100

0.815
  radA Bacillus subtilis subsp. subtilis str. 168

64.68

100

0.647

Multiple sequence alignment