Detailed information NTG0246159

Bioinformatically predicted

Overview

• Name: rpoS
• Type: Regulator
• Locus tag: AOY85_RS17460
• Genome accession: NZ_CP028743
• Coordinates: 3610177..3611169 (-)
• Length: 330 a.a.
• NCBI ID: WP_000081550.1
• Uniprot ID: A0A370V765
• Organism: Escherichia coli strain A32
• Function: regulation of chitinases (predicted from homology) Competence regulation

Genomic Context

Location: 3605177..3616169

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AOY85_RS17440 (AOY85_18490)	ygbM	3605814..3606590 (+)	777	WP_001136915.1	HPr family phosphocarrier protein	-
AOY85_RS17445 (AOY85_18495)	ygbN	3606679..3608043 (+)	1365	WP_000104433.1	GntP family transporter	-
AOY85_RS17450 (AOY85_18500)	-	3608186..3609230 (-)	1045	Protein_3401	MBL fold metallo-hydrolase	-
AOY85_RS17455 (AOY85_18505)	-	3609455..3610006 (+)	552	WP_001179443.1	flavodoxin family protein	-
AOY85_RS17460 (AOY85_18510)	rpoS	3610177..3611169 (-)	993	WP_000081550.1	RNA polymerase sigma factor RpoS	Regulator
AOY85_RS17465 (AOY85_18515)	nlpD	3611232..3612326 (-)	1095	WP_306217602.1	murein hydrolase activator NlpD	-
AOY85_RS17470 (AOY85_18520)	pcm	3612511..3613137 (-)	627	WP_000254708.1	protein-L-isoaspartate O-methyltransferase	-
AOY85_RS17475 (AOY85_18525)	surE	3613131..3613892 (-)	762	WP_001350156.1	5'/3'-nucleotidase SurE	-
AOY85_RS17480 (AOY85_18530)	truD	3613873..3614922 (-)	1050	WP_000568929.1	tRNA pseudouridine(13) synthase TruD	-
AOY85_RS17485 (AOY85_18535)	ispF	3614919..3615398 (-)	480	WP_001219238.1	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase	-
AOY85_RS17490 (AOY85_18540)	ispD	3615398..3616108 (-)	711	WP_000246149.1	2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase	-

Sequence

Protein

• Length: 330 a.a.
• Molecular weight: 37972.84 Da
• Isoelectric Point: 4.5597

>NTDB_id=246159 AOY85_RS17460 WP_000081550.1 3610177..3611169(-) (rpoS) [Escherichia coli strain A32]
MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEELLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFA
RRALRGDVASRRRMIESNLRLVVKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNERITSVDTPLGGDSEKALLDILAD
EKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLARRFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQ
GLNIEALFRE

Nucleotide

• Length: 993 bp

>NTDB_id=246159 AOY85_RS17460 WP_000081550.1 3610177..3611169(-) (rpoS) [Escherichia coli strain A32]
ATGAGTCAGAATACGCTGAAAGTTCATGATTTAAATGAAGATGCGGAATTTGATGAGAACGGAGTTGAGGTTTTTGACGA
AAAGGCCTTAGTAGAAGAGGAACCCAGTGATAACGATTTGGCCGAAGAGGAACTGTTATCGCAGGGAGCCACACAGCGTG
TGCTGGACGCGACTCAGCTTTACCTTGGTGAGATTGGTTATTCACCACTGTTAACGGCCGAAGAAGAAGTTTATTTTGCG
CGTCGCGCACTGCGTGGAGATGTCGCCTCTCGCCGCCGGATGATCGAGAGTAACTTGCGTCTGGTGGTAAAAATTGCCCG
CCGTTATGGCAATCGTGGTCTGGCGTTGCTGGACCTGATCGAAGAGGGCAACCTGGGGCTGATCCGCGCGGTAGAGAAGT
TTGACCCGGAACGTGGTTTCCGCTTCTCAACATACGCAACCTGGTGGATTCGCCAGACGATCGAACGGGCGATTATGAAC
CAAACCCGTACTATTCGTTTGCCGATTCACATCGTAAAGGAGCTGAACGTTTACCTGCGAACCGCACGTGAGTTGTCCCA
TAAGCTGGACCACGAACCAAGTGCGGAAGAGATCGCAGAGCAACTGGATAAGCCAGTTGATGACGTCAGCCGTATGCTTC
GTCTTAACGAGCGCATTACCTCGGTAGACACCCCGCTGGGTGGTGATTCCGAAAAAGCGTTGCTGGACATCCTGGCCGAT
GAAAAAGAGAATGGTCCGGAAGATACCACGCAAGATGACGATATGAAGCAGAGCATCGTCAAATGGCTGTTCGAGCTGAA
CGCCAAACAGCGTGAAGTGCTGGCACGTCGATTCGGTTTGCTGGGGTACGAAGCGGCAACACTGGAAGATGTAGGTCGTG
AAATTGGCCTCACCCGTGAACGTGTTCGCCAGATTCAGGTTGAAGGCCTGCGCCGTTTGCGCGAAATCCTGCAAACGCAG
GGGCTGAATATCGAAGCGCTGTTCCGCGAGTAA

Domains

Predicted by InterProScan.

• Sigma-70 region 3 (Sigma70_r3) (174-248)

• Sigma-70 region 2 (Sigma70_r2) (94-163)

• Sigma-70 factor, region 1.2 (Sigma70_r1_2) (56-88)

• Sigma-70, region 4 (Sigma70_r4) (262-315)

3D structure

• AlphaFold DB: A0A370V765

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rpoS	Vibrio cholerae O1 biovar El Tor strain E7946	73.065	97.879	0.715