Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   CLI97_RS12425 Genome accession   NZ_CP023320
Coordinates   2338254..2338691 (-) Length   145 a.a.
NCBI ID   WP_003156588.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2333254..2343691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS21305 - 2333843..2333968 (-) 126 Protein_2399 hypothetical protein -
  CLI97_RS12390 (CLI97_02448) - 2334121..2335323 (+) 1203 WP_043866820.1 GTP-binding protein -
  CLI97_RS12395 (CLI97_02449) - 2335388..2336524 (+) 1137 WP_077721769.1 zinc-dependent alcohol dehydrogenase -
  CLI97_RS12400 (CLI97_02450) - 2336539..2336973 (+) 435 WP_021494203.1 RDD family protein -
  CLI97_RS12405 (CLI97_02451) - 2337091..2337366 (+) 276 WP_369719109.1 YckD family protein -
  CLI97_RS21535 (CLI97_02452) - 2337470..2337661 (+) 192 WP_071392145.1 family 1 glycosylhydrolase -
  CLI97_RS12415 - 2337586..2337815 (+) 230 Protein_2405 family 1 glycosylhydrolase -
  CLI97_RS12420 (CLI97_02453) nin/comJ 2337834..2338232 (-) 399 WP_094247249.1 competence protein ComJ Regulator
  CLI97_RS12425 (CLI97_02454) nucA/comI 2338254..2338691 (-) 438 WP_003156588.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  CLI97_RS12430 (CLI97_02455) hxlB 2339052..2339609 (-) 558 WP_014304318.1 6-phospho-3-hexuloisomerase -
  CLI97_RS12435 (CLI97_02456) hxlA 2339606..2340241 (-) 636 WP_014304319.1 3-hexulose-6-phosphate synthase -
  CLI97_RS12440 (CLI97_02457) - 2340473..2340835 (+) 363 WP_014304320.1 helix-turn-helix domain-containing protein -

Sequence

Protein


Download         Length: 145 a.a.        Molecular weight: 16072.35 Da        Isoelectric Point: 8.4850

>NTDB_id=246051 CLI97_RS12425 WP_003156588.1 2338254..2338691(-) (nucA/comI) [Bacillus velezensis strain SCGB]
MKTLKALLLVLIIIAGAVAALIKGDLFSDNEQTVQKSDYDKVILFPSDRYPETAKHIKDAIKEGHSSICTIDRDGVHKRR
EQSLKHVPVKTGYDRDEWPMAVCKQGGNNASVEYISPADNRGAGSWVGHQLTNDPDGTRVLFKIK

Nucleotide


Download         Length: 438 bp        

>NTDB_id=246051 CLI97_RS12425 WP_003156588.1 2338254..2338691(-) (nucA/comI) [Bacillus velezensis strain SCGB]
TTGAAAACATTAAAAGCACTGCTGCTTGTTCTTATCATCATTGCCGGAGCAGTCGCGGCCCTGATCAAGGGTGATCTTTT
CTCAGATAACGAGCAGACCGTTCAGAAGAGTGATTACGACAAAGTAATCCTATTTCCGTCCGATCGATATCCGGAAACAG
CAAAGCATATTAAGGACGCGATAAAAGAAGGGCATTCCAGCATATGCACCATTGACAGAGACGGAGTACATAAGCGGCGT
GAGCAATCTCTTAAACATGTGCCTGTCAAAACGGGATACGATAGGGATGAATGGCCGATGGCGGTTTGTAAACAGGGCGG
AAACAATGCCTCCGTAGAATATATTTCTCCGGCAGACAACCGCGGAGCCGGCTCTTGGGTCGGACACCAGCTTACCAATG
ATCCCGACGGAACGAGAGTATTATTTAAAATCAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(49-131)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

73.81

86.897

0.641

Multiple sequence alignment