Detailed information    

insolico Bioinformatically predicted

Overview

Name   recF   Type   Machinery gene
Locus tag   CLI97_RS10640 Genome accession   NZ_CP023320
Coordinates   2010088..2011200 (+) Length   370 a.a.
NCBI ID   WP_094247314.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1990066..2036377 2010088..2011200 within 0


Gene organization within MGE regions


Location: 1990066..2036377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS10535 (CLI97_02083) ssbA 1990066..1990584 (-) 519 WP_004392979.1 single-stranded DNA-binding protein SsbA Machinery gene
  CLI97_RS10540 (CLI97_02084) rpsF 1990624..1990911 (-) 288 WP_004392977.1 30S ribosomal protein S6 -
  CLI97_RS10545 (CLI97_02085) ychF 1991023..1992123 (-) 1101 WP_004392975.1 redox-regulated ATPase YchF -
  CLI97_RS10550 (CLI97_02086) - 1992250..1994253 (-) 2004 WP_094247316.1 molybdopterin-dependent oxidoreductase -
  CLI97_RS10555 (CLI97_02087) - 1994300..1994506 (-) 207 WP_004392973.1 DUF951 domain-containing protein -
  CLI97_RS10560 (CLI97_02088) - 1994592..1995617 (-) 1026 WP_088005468.1 hypothetical protein -
  CLI97_RS10565 (CLI97_02089) - 1995785..1996252 (+) 468 WP_223883518.1 GNAT family N-acetyltransferase -
  CLI97_RS10570 (CLI97_02090) yyaC 1996427..1997041 (+) 615 WP_004392969.1 spore protease YyaC -
  CLI97_RS10575 (CLI97_02091) - 1997070..1997921 (-) 852 WP_004392966.1 ParB/RepB/Spo0J family partition protein -
  CLI97_RS10580 (CLI97_02092) soj 1997914..1998675 (-) 762 WP_007409899.1 sporulation initiation inhibitor protein Soj -
  CLI97_RS10585 (CLI97_02093) noc 1998974..1999825 (-) 852 WP_007409900.1 nucleoid occlusion protein -
  CLI97_RS10590 (CLI97_02094) rsmG 1999947..2000666 (-) 720 WP_014306213.1 16S rRNA (guanine(527)-N(7))-methyltransferase RsmG -
  CLI97_RS10595 (CLI97_02095) mnmG 2000680..2002566 (-) 1887 WP_004392959.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis enzyme MnmG -
  CLI97_RS10600 (CLI97_02096) mnmE 2002583..2003962 (-) 1380 WP_004392954.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  CLI97_RS10605 (CLI97_02097) jag 2004252..2004893 (-) 642 WP_017418434.1 RNA-binding cell elongation regulator Jag/EloR -
  CLI97_RS10610 (CLI97_02098) spoIIIJ 2004890..2005639 (-) 750 WP_071182106.1 YidC family membrane integrase SpoIIIJ -
  CLI97_RS10615 (CLI97_02099) rnpA 2005800..2006150 (-) 351 WP_004392944.1 ribonuclease P protein component -
  CLI97_RS10620 rpmH 2006305..2006439 (-) 135 WP_003178075.1 50S ribosomal protein L34 -
  CLI97_RS10625 (CLI97_02100) dnaA 2007061..2008401 (+) 1341 WP_004392913.1 chromosomal replication initiator protein DnaA -
  CLI97_RS10630 (CLI97_02101) dnaN 2008586..2009722 (+) 1137 WP_094247315.1 DNA polymerase III subunit beta -
  CLI97_RS10635 (CLI97_02102) rlbA 2009857..2010072 (+) 216 WP_004392910.1 ribosome maturation protein RlbA -
  CLI97_RS10640 (CLI97_02103) recF 2010088..2011200 (+) 1113 WP_094247314.1 DNA replication/repair protein RecF Machinery gene
  CLI97_RS10645 (CLI97_02104) remB 2011218..2011463 (+) 246 WP_004392908.1 extracellular matrix regulator RemB -
  CLI97_RS10650 (CLI97_02105) gyrB 2011523..2013439 (+) 1917 WP_004392900.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  CLI97_RS10655 (CLI97_02106) gyrA 2013655..2016114 (+) 2460 WP_004392898.1 DNA gyrase subunit A -
  CLI97_RS10685 (CLI97_02112) - 2021453..2022403 (-) 951 WP_025854090.1 YaaC family protein -
  CLI97_RS10690 (CLI97_02113) guaB 2022524..2023990 (+) 1467 WP_003150717.1 IMP dehydrogenase -
  CLI97_RS10695 (CLI97_02114) dacA 2024143..2025474 (+) 1332 WP_077721682.1 D-alanyl-D-alanine carboxypeptidase -
  CLI97_RS10700 (CLI97_02115) pdxS 2025672..2026556 (+) 885 WP_003150714.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
  CLI97_RS10705 (CLI97_02116) pdxT 2026578..2027168 (+) 591 WP_007615126.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
  CLI97_RS10710 (CLI97_02117) serS 2027488..2028765 (+) 1278 WP_003150709.1 serine--tRNA ligase -
  CLI97_RS10715 (CLI97_02118) - 2028777..2029922 (-) 1146 WP_094247313.1 glycerate kinase -
  CLI97_RS10725 (CLI97_02120) dck 2030363..2031016 (-) 654 WP_015388792.1 deoxyadenosine/deoxycytidine kinase -
  CLI97_RS10730 (CLI97_02121) - 2031013..2031636 (-) 624 WP_094247312.1 deoxynucleoside kinase -
  CLI97_RS10735 (CLI97_02122) - 2031732..2033015 (-) 1284 WP_032865795.1 LysM peptidoglycan-binding domain-containing protein -
  CLI97_RS10740 (CLI97_02123) - 2033087..2033599 (-) 513 WP_046376732.1 cysteine hydrolase family protein -
  CLI97_RS10745 (CLI97_02124) tadA 2033721..2034203 (+) 483 WP_007615141.1 tRNA adenosine(34) deaminase TadA -
  CLI97_RS10755 (CLI97_02125) dnaX 2034686..2036377 (+) 1692 WP_096034914.1 DNA polymerase III subunit gamma/tau -

Sequence

Protein


Download         Length: 370 a.a.        Molecular weight: 42400.34 Da        Isoelectric Point: 7.0882

>NTDB_id=246044 CLI97_RS10640 WP_094247314.1 2010088..2011200(+) (recF) [Bacillus velezensis strain SCGB]
MYIQNLELTSYRNYERAELQFENKVNVIIGENAQGKTNLMEAIYVLSMAKSHRTSNDKELIRWDEDYAKIEGRVMKRNGD
IPMQLVISKKGKKGKVNHIEQQKLSQYVGALNTIMFAPEDLNLVKGSPQVRRRFLDMEIGQVSAVYLYDLSLYQKILSQR
NHFLKQLQSRKQTDRTMLDVLTDQLIEAAAKVVAKRLQFTAQLEKWAQPIHSGISRGLEELTLKYHTALDVSDPKDLSKI
GNSYQESFSKLKEKEIERGVTLFGPHRDDVLFYVNGRDVQTYGSQGQQRTTALSLKLAEIDLIHEEIGEYPILLLDDVLS
ELDDYRQSHLLHTIQGRVQTFVTTTSVDGIDHDTLHQAGMFRVENGALVK

Nucleotide


Download         Length: 1113 bp        

>NTDB_id=246044 CLI97_RS10640 WP_094247314.1 2010088..2011200(+) (recF) [Bacillus velezensis strain SCGB]
TTGTATATCCAAAATTTAGAATTAACATCCTACCGTAATTATGAGCGCGCTGAGCTTCAATTCGAAAACAAGGTGAATGT
GATCATCGGGGAAAATGCCCAGGGCAAAACCAATCTGATGGAAGCCATCTATGTCCTTTCAATGGCAAAATCACACCGCA
CCTCAAATGACAAAGAACTTATACGGTGGGACGAAGACTATGCTAAAATAGAAGGAAGAGTGATGAAGCGCAACGGGGAC
ATCCCGATGCAGCTTGTCATCTCCAAAAAAGGTAAAAAGGGCAAGGTCAATCATATAGAGCAGCAAAAACTCAGTCAGTA
TGTCGGAGCGCTGAACACCATTATGTTTGCCCCTGAGGATTTAAATCTCGTCAAAGGAAGCCCTCAGGTGAGGAGAAGAT
TTCTCGATATGGAGATCGGGCAGGTTTCTGCCGTTTATCTGTATGATTTATCTCTTTATCAAAAAATCCTTTCACAGCGG
AATCATTTTCTGAAACAGCTGCAAAGCCGGAAACAGACTGACCGGACCATGCTTGACGTTCTCACTGATCAGCTGATTGA
AGCAGCCGCAAAAGTTGTCGCCAAACGTCTGCAGTTTACGGCGCAGCTTGAAAAATGGGCGCAGCCGATCCATTCCGGCA
TTTCCCGGGGACTTGAAGAACTGACGCTTAAGTATCACACGGCGCTTGATGTATCAGATCCTAAAGATTTGTCGAAAATA
GGTAACAGCTACCAGGAGTCTTTTTCGAAATTAAAAGAAAAAGAAATAGAACGAGGCGTCACATTATTCGGACCGCATCG
GGATGATGTCCTTTTTTATGTGAACGGACGCGATGTGCAGACTTACGGTTCGCAGGGACAGCAGCGTACGACGGCTTTGT
CTCTGAAGCTGGCTGAAATAGATTTGATTCATGAAGAAATCGGAGAATACCCCATTTTACTTTTGGATGATGTACTAAGC
GAGCTGGATGATTACCGACAGTCCCATTTGCTTCATACGATCCAAGGCCGTGTGCAGACATTTGTCACCACGACGAGCGT
GGATGGCATTGACCACGATACCTTACATCAAGCAGGAATGTTCCGTGTGGAAAACGGTGCGTTAGTGAAGTGA

Domains

Predicted by InterproScan.

RecF/RecN/SMC N terminal domain (SMC_N)

(2-346)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recF Bacillus subtilis subsp. subtilis str. 168

94.595

100

0.946

Multiple sequence alignment