Detailed information    

insolico Bioinformatically predicted

Overview

Name   recU   Type   Machinery gene
Locus tag   CLI97_RS01175 Genome accession   NZ_CP023320
Coordinates   187786..188394 (+) Length   202 a.a.
NCBI ID   WP_094247938.1    Uniprot ID   -
Organism   Bacillus velezensis strain SCGB     
Function   plasmid transformation; homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 182786..193394
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CLI97_RS01135 (CLI97_00225) - 183872..184378 (-) 507 WP_012117833.1 PTS glucose transporter subunit IIA -
  CLI97_RS01140 (CLI97_00226) - 184517..184930 (+) 414 WP_076851379.1 hypothetical protein -
  CLI97_RS01145 (CLI97_00227) - 184960..185361 (-) 402 WP_094247839.1 YppG family protein -
  CLI97_RS01150 (CLI97_00228) - 185548..185730 (+) 183 WP_003153532.1 YppF family protein -
  CLI97_RS01155 (CLI97_00229) - 185769..186137 (-) 369 WP_003153529.1 YppE family protein -
  CLI97_RS01160 (CLI97_00230) - 186183..186428 (-) 246 WP_094247838.1 DUF5446 family protein -
  CLI97_RS01165 (CLI97_00231) sspM 186634..186738 (+) 105 WP_003153525.1 acid-soluble spore protein SspM -
  CLI97_RS01170 (CLI97_00232) - 186782..187744 (-) 963 WP_032861546.1 DUF2515 domain-containing protein -
  CLI97_RS01175 (CLI97_00233) recU 187786..188394 (+) 609 WP_094247938.1 Holliday junction resolvase RecU Machinery gene
  CLI97_RS01180 (CLI97_00234) - 188433..191216 (+) 2784 WP_070082018.1 PBP1A family penicillin-binding protein -
  CLI97_RS01185 (CLI97_00235) - 191295..191786 (-) 492 WP_046559838.1 YpoC family protein -
  CLI97_RS01190 (CLI97_00236) nth 191783..192442 (-) 660 WP_094247837.1 endonuclease III -
  CLI97_RS01195 (CLI97_00237) dnaD 192461..193159 (-) 699 WP_094247836.1 DNA replication protein DnaD -

Sequence

Protein


Download         Length: 202 a.a.        Molecular weight: 23708.84 Da        Isoelectric Point: 9.6948

>NTDB_id=246005 CLI97_RS01175 WP_094247938.1 187786..188394(+) (recU) [Bacillus velezensis strain SCGB]
MIRYPNGKTFQPNKTVSSQNSQKRSHSYSNRGMTLEDDLNETNKYYLANQIAVIHKRPTPVQIVNVHYPKRSAAVIKEAY
FKQSSTTDYNGIYKGRYIDFEAKETKNKTAFPLQNFHDHQIEHMKQVVRQDGICFVIISAFGKVYFLEADKLFVFWERKE
KNGRKSIRKDELEDASFPISLGYSPRIDYISIIEQLYFSQEQ

Nucleotide


Download         Length: 609 bp        

>NTDB_id=246005 CLI97_RS01175 WP_094247938.1 187786..188394(+) (recU) [Bacillus velezensis strain SCGB]
GTGATTCGGTACCCGAATGGAAAAACTTTTCAGCCGAACAAAACGGTTTCATCCCAAAACAGCCAGAAACGGTCTCATTC
ATACAGTAATCGCGGCATGACCCTCGAAGACGACCTGAATGAAACGAATAAGTATTATCTGGCTAACCAAATTGCTGTCA
TTCACAAAAGGCCGACACCCGTCCAGATTGTAAACGTCCACTATCCGAAAAGAAGCGCCGCAGTCATTAAGGAAGCTTAT
TTTAAACAGTCTTCAACAACTGACTACAACGGCATTTATAAAGGACGCTACATTGATTTCGAGGCAAAAGAAACAAAAAA
CAAAACGGCATTTCCCCTGCAGAATTTTCATGATCATCAGATTGAGCATATGAAGCAGGTGGTCAGGCAAGACGGCATTT
GTTTTGTTATTATATCCGCTTTCGGAAAGGTTTATTTCCTCGAAGCCGACAAGCTGTTTGTCTTTTGGGAGAGAAAGGAG
AAGAACGGCAGAAAATCAATTCGCAAAGACGAATTAGAAGATGCTTCCTTTCCGATTTCCCTTGGATACTCACCGAGAAT
TGATTATATTAGTATTATTGAACAGCTTTATTTTTCGCAGGAACAGTAG

Domains

Predicted by InterproScan.

Recombination protein U (RecU)

(31-193)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recU Bacillus subtilis subsp. subtilis str. 168

90.452

98.515

0.891

Multiple sequence alignment